Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103478
Name   oriT_AGR_R10|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_R10
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039216 (22277..22359 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_R10|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAAAAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2601 GenBank   WP_241391181
Name   Relaxase_MOP20_RS00135_AGR_R10|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73412.67 Da        Isoelectric Point: 7.7760

>WP_241391181.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPKTYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYMRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2401 GenBank   WP_241391186
Name   trbC_MOP20_RS00175_AGR_R10|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81543.39 Da        Isoelectric Point: 8.3675

>WP_241391186.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKF
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPIAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33053..61345

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP20_RS00165 (R10p_00040) 29331..30275 + 945 WP_241391185 hypothetical protein -
MOP20_RS00170 (R10p_00041) 30411..30689 - 279 Protein_34 helix-turn-helix domain-containing protein -
MOP20_RS00175 (R10p_00042) 30838..33015 - 2178 WP_241391186 F-type conjugative transfer protein TrbC -
MOP20_RS00180 (R10p_00043) 33053..34051 - 999 WP_010895863 hypothetical protein trbB
MOP20_RS00185 (R10p_00044) 34064..35323 - 1260 WP_241391187 conjugal transfer protein TrbA trbA
MOP20_RS00190 (R10p_00045) 35513..36094 - 582 WP_241391188 hypothetical protein -
MOP20_RS00195 (R10p_00046) 36091..38364 - 2274 WP_241391189 DotA/TraY family protein traY
MOP20_RS00200 (R10p_00047) 38300..38863 - 564 WP_241391190 conjugal transfer protein TraX -
MOP20_RS00205 (R10p_00048) 38856..40082 - 1227 WP_241389873 conjugal transfer protein TraW traW
MOP20_RS00210 (R10p_00050) 40223..43297 - 3075 WP_241389874 type IV secretory system conjugative DNA transfer family protein traU
MOP20_RS00215 (R10p_00051) 43300..43905 - 606 WP_241391191 hypothetical protein traT
MOP20_RS00220 (R10p_00052) 43992..44393 - 402 WP_241389876 DUF6750 family protein traR
MOP20_RS00225 (R10p_00053) 44409..44939 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOP20_RS00230 (R10p_00054) 44948..45691 - 744 WP_241391192 hypothetical protein traP
MOP20_RS00235 (R10p_00055) 45691..46923 - 1233 WP_241391193 conjugal transfer protein TraO traO
MOP20_RS00240 (R10p_00056) 46927..47934 - 1008 WP_010895741 DotH/IcmK family type IV secretion protein traN
MOP20_RS00245 (R10p_00057) 47937..48611 - 675 WP_010895742 DotI/IcmL/TraM family protein traM
MOP20_RS00250 (R10p_00058) 48702..49193 - 492 WP_010895743 hypothetical protein traL
MOP20_RS00255 (R10p_00059) 49203..52439 - 3237 WP_241391194 LPD7 domain-containing protein -
MOP20_RS00260 (R10p_00061) 52582..52848 - 267 WP_241389612 IcmT/TraK family protein traK
MOP20_RS00265 (R10p_00062) 52848..54005 - 1158 WP_241389613 plasmid transfer ATPase TraJ virB11
MOP20_RS00270 (R10p_00064) 54195..54983 - 789 WP_241389822 type IV secretory system conjugative DNA transfer family protein traI
MOP20_RS00275 (R10p_00065) 54980..55438 - 459 WP_241389823 DotD/TraH family lipoprotein -
MOP20_RS00280 (R10p_00066) 55476..56909 - 1434 WP_241389824 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP20_RS00285 (R10p_00067) 56906..57568 - 663 WP_241390363 prepilin peptidase -
MOP20_RS00290 (R10p_00068) 57574..58059 - 486 WP_241389826 lytic transglycosylase domain-containing protein virB1
MOP20_RS00295 (R10p_00069) 58114..58707 - 594 WP_010895752 type 4 pilus major pilin -
MOP20_RS00300 (R10p_00070) 58743..59855 - 1113 WP_241389827 type II secretion system F family protein -
MOP20_RS00305 (R10p_00071) 59855..61345 - 1491 WP_241391166 ATPase, T2SS/T4P/T4SS family virB11
MOP20_RS00310 (R10p_00072) 61351..61935 - 585 WP_241389829 type IV pilus biogenesis protein PilP -
MOP20_RS00315 (R10p_00073) 61922..63232 - 1311 WP_241389830 type 4b pilus protein PilO2 -
MOP20_RS00320 (R10p_00074) 63236..64870 - 1635 WP_241391167 PilN family type IVB pilus formation outer membrane protein -
MOP20_RS00325 (R10p_00075) 64895..65320 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOP20_RS00330 (R10p_00076) 65320..66264 - 945 WP_241389832 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3921 GenBank   NZ_MT039216
Plasmid name   AGR_R10|unnamed1 Incompatibility group   -
Plasmid size   102745 bp Coordinate of oriT [Strand]   22277..22359 [+]
Host baterium   Serratia proteamaculans strain AGR_R10

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -