Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103476
Name   oriT_AGR_SpF|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_SpF
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039222 (21192..21273 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_AGR_SpF|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGTGCCCCGACACCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2599 GenBank   WP_241389589
Name   Relaxase_MOP28_RS00135_AGR_SpF|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73493.72 Da        Isoelectric Point: 7.4608

>WP_241389589.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDDDKPDTPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVGLQQI
MHAEPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKEKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
SGMALAENKSGEYLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)


  Protein structure



No available structure.




T4CP

ID   2399 GenBank   WP_241389595
Name   trbC_MOP28_RS00175_AGR_SpF|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81541.37 Da        Isoelectric Point: 8.3675

>WP_241389595.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDILHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32070..60376

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP28_RS00165 (SpFp1_00063) 28279..29223 + 945 WP_241389593 hypothetical protein -
MOP28_RS00170 (SpFp1_00062) 29359..29844 - 486 WP_241389594 phospholipase D-like domain-containing protein -
MOP28_RS00175 (SpFp1_00061) 29855..32032 - 2178 WP_241389595 F-type conjugative transfer protein TrbC -
MOP28_RS00180 (SpFp1_00060) 32070..33068 - 999 WP_241389596 hypothetical protein trbB
MOP28_RS00185 (SpFp1_00059) 33080..34390 - 1311 WP_241389597 conjugal transfer protein TrbA trbA
MOP28_RS00190 (SpFp1_00058) 34529..35071 - 543 WP_241389598 hypothetical protein -
MOP28_RS00195 (SpFp1_00057) 35107..37251 - 2145 WP_241389599 DotA/TraY family protein traY
MOP28_RS00200 (SpFp1_00056) 37316..37888 - 573 WP_241389600 conjugal transfer protein TraX -
MOP28_RS00205 (SpFp1_00055) 37881..39107 - 1227 WP_241389601 conjugal transfer protein TraW traW
MOP28_RS00210 (SpFp1_00053) 39248..42322 - 3075 WP_241389602 type IV secretory system conjugative DNA transfer family protein traU
MOP28_RS00215 (SpFp1_00052) 42325..42930 - 606 WP_241389603 hypothetical protein traT
MOP28_RS00220 (SpFp1_00051) 43017..43418 - 402 WP_241389604 DUF6750 family protein traR
MOP28_RS00225 (SpFp1_00050) 43434..43964 - 531 WP_241389605 conjugal transfer protein TraQ traQ
MOP28_RS00230 (SpFp1_00049) 43973..44722 - 750 WP_241389606 hypothetical protein traP
MOP28_RS00235 (SpFp1_00048) 44722..45954 - 1233 WP_241389607 conjugal transfer protein TraO traO
MOP28_RS00240 (SpFp1_00047) 45958..46965 - 1008 WP_241389608 DotH/IcmK family type IV secretion protein traN
MOP28_RS00245 (SpFp1_00046) 46968..47654 - 687 WP_241389609 DotI/IcmL/TraM family protein traM
MOP28_RS00250 (SpFp1_00045) 47733..48224 - 492 WP_241389610 hypothetical protein traL
MOP28_RS00255 (SpFp1_00044) 48234..51470 - 3237 WP_241389611 LPD7 domain-containing protein -
MOP28_RS00260 (SpFp1_00042) 51613..51879 - 267 WP_241389612 IcmT/TraK family protein traK
MOP28_RS00265 (SpFp1_00041) 51879..53036 - 1158 WP_241389613 plasmid transfer ATPase TraJ virB11
MOP28_RS00270 (SpFp1_00039) 53226..54014 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP28_RS00275 (SpFp1_00038) 54011..54469 - 459 WP_241389614 DotD/TraH family lipoprotein -
MOP28_RS00280 (SpFp1_00037) 54507..55940 - 1434 WP_241389615 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP28_RS00285 (SpFp1_00036) 55937..56605 - 669 WP_241389616 prepilin peptidase -
MOP28_RS00290 (SpFp1_00035) 56605..57090 - 486 WP_241389617 lytic transglycosylase domain-containing protein virB1
MOP28_RS00295 (SpFp1_00034) 57145..57738 - 594 WP_010895752 type 4 pilus major pilin -
MOP28_RS00300 (SpFp1_00033) 57774..58889 - 1116 WP_241391164 type II secretion system F family protein -
MOP28_RS00305 (SpFp1_00032) 58886..60376 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOP28_RS00310 (SpFp1_00031) 60382..60966 - 585 WP_241389531 type IV pilus biogenesis protein PilP -
MOP28_RS00315 (SpFp1_00030) 60953..62263 - 1311 WP_241389532 type 4b pilus protein PilO2 -
MOP28_RS00320 (SpFp1_00029) 62267..63901 - 1635 WP_241389533 PilN family type IVB pilus formation outer membrane protein -
MOP28_RS00325 (SpFp1_00028) 63926..64351 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOP28_RS00330 (SpFp1_00027) 64351..65364 - 1014 WP_241389534 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3919 GenBank   NZ_MT039222
Plasmid name   AGR_SpF|unnamed1 Incompatibility group   -
Plasmid size   113377 bp Coordinate of oriT [Strand]   21192..21273 [+]
Host baterium   Serratia proteamaculans strain AGR_SpF

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -