Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103474
Name   oriT_AGR_1772|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_1772
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039200 (22632..22714 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_1772|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAAGGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2597 GenBank   WP_241391276
Name   Relaxase_MOP25_RS00150_AGR_1772|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73413.62 Da        Isoelectric Point: 7.4747

>WP_241391276.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVDLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKVADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVSECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARGAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYMRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2397 GenBank   WP_241390158
Name   trbC_MOP25_RS00190_AGR_1772|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81527.35 Da        Isoelectric Point: 8.3675

>WP_241390158.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33510..61789

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP25_RS00180 (1772p1_00077) 29719..30663 + 945 WP_241389593 hypothetical protein -
MOP25_RS00185 (1772p1_00078) 30799..31284 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOP25_RS00190 (1772p1_00079) 31295..33472 - 2178 WP_241390158 F-type conjugative transfer protein TrbC -
MOP25_RS00195 (1772p1_00080) 33510..34508 - 999 WP_241390308 hypothetical protein trbB
MOP25_RS00200 (1772p1_00081) 34521..35831 - 1311 WP_241390430 conjugal transfer protein TrbA trbA
MOP25_RS00205 (1772p1_00082) 35960..36538 - 579 WP_241389923 hypothetical protein -
MOP25_RS00210 (1772p1_00083) 36535..38829 - 2295 WP_261097221 DotA/TraY family protein -
MOP25_RS00215 (1772p1_00084) 38744..39307 - 564 WP_241390311 conjugal transfer protein TraX -
MOP25_RS00220 (1772p1_00085) 39300..40526 - 1227 WP_241390312 conjugal transfer protein TraW traW
MOP25_RS00225 (1772p1_00087) 40667..43741 - 3075 WP_241390313 type IV secretory system conjugative DNA transfer family protein traU
MOP25_RS00230 (1772p1_00088) 43744..44349 - 606 WP_241389875 hypothetical protein traT
MOP25_RS00235 (1772p1_00089) 44436..44837 - 402 WP_241389604 DUF6750 family protein traR
MOP25_RS00240 (1772p1_00090) 44853..45383 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOP25_RS00245 (1772p1_00091) 45392..46135 - 744 WP_241390314 hypothetical protein traP
MOP25_RS00250 (1772p1_00092) 46135..47367 - 1233 WP_241389929 conjugal transfer protein TraO traO
MOP25_RS00255 (1772p1_00093) 47371..48378 - 1008 WP_241390316 DotH/IcmK family type IV secretion protein traN
MOP25_RS00260 (1772p1_00094) 48381..49055 - 675 WP_241390317 DotI/IcmL/TraM family protein traM
MOP25_RS00265 (1772p1_00095) 49146..49637 - 492 WP_010895743 hypothetical protein traL
MOP25_RS00270 (1772p1_00096) 49647..52883 - 3237 WP_241390318 LPD7 domain-containing protein -
MOP25_RS00275 (1772p1_00098) 53026..53292 - 267 WP_241390319 IcmT/TraK family protein traK
MOP25_RS00280 (1772p1_00099) 53292..54449 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOP25_RS00285 (1772p1_00101) 54639..55427 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP25_RS00290 (1772p1_00102) 55424..55882 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP25_RS00295 (1772p1_00103) 55920..57353 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP25_RS00300 (1772p1_00104) 57350..58018 - 669 WP_241390170 prepilin peptidase -
MOP25_RS00305 (1772p1_00105) 58018..58503 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP25_RS00310 (1772p1_00106) 58558..59151 - 594 WP_010895752 type 4 pilus major pilin -
MOP25_RS00315 (1772p1_00107) 59187..60299 - 1113 WP_241389818 type II secretion system F family protein -
MOP25_RS00320 (1772p1_00108) 60299..61789 - 1491 WP_241389888 ATPase, T2SS/T4P/T4SS family virB11
MOP25_RS00325 (1772p1_00109) 61795..62379 - 585 WP_241389889 type IV pilus biogenesis protein PilP -
MOP25_RS00330 (1772p1_00110) 62366..63676 - 1311 WP_241390279 type 4b pilus protein PilO2 -
MOP25_RS00335 (1772p1_00111) 63680..65314 - 1635 WP_241389891 PilN family type IVB pilus formation outer membrane protein -
MOP25_RS00340 (1772p1_00112) 65336..65761 - 426 WP_241389892 type IV pilus biogenesis protein PilM -
MOP25_RS00345 (1772p1_00113) 65761..66774 - 1014 WP_241390280 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3917 GenBank   NZ_MT039200
Plasmid name   AGR_1772|unnamed1 Incompatibility group   -
Plasmid size   141827 bp Coordinate of oriT [Strand]   22632..22714 [+]
Host baterium   Serratia proteamaculans strain AGR_1772

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -