Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103472
Name   oriT_AGR_Puna18|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_Puna18
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039215 (22671..22753 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_Puna18|unnamed1
ACGGGACAAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2595 GenBank   WP_241391494
Name   Relaxase_MOP38_RS00150_AGR_Puna18|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73409.61 Da        Isoelectric Point: 7.6200

>WP_241391494.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYRSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2395 GenBank   WP_272491693
Name   trbC_MOP38_RS00165_AGR_Puna18|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81479.29 Da        Isoelectric Point: 8.3675

>WP_272491693.1 F-type conjugative transfer protein TrbC [Serratia proteamaculans]
MSRPVEVDPRRVRRPLGYTFINDALLSPIGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 28217..56490

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP38_RS00155 (Puna18p_00050) 25122..25445 - 324 WP_241391495 hypothetical protein -
MOP38_RS00160 (Puna18p_00049) 25455..25979 - 525 WP_241391496 phospholipase D family protein -
MOP38_RS00165 (Puna18p_00048) 26002..28179 - 2178 WP_272491693 F-type conjugative transfer protein TrbC -
MOP38_RS00170 (Puna18p_00047) 28217..29215 - 999 WP_241391498 hypothetical protein trbB
MOP38_RS00175 (Puna18p_00046) 29227..30537 - 1311 WP_241391499 conjugal transfer protein TrbA trbA
MOP38_RS00180 (Puna18p_00045) 30666..31244 - 579 WP_241389923 hypothetical protein -
MOP38_RS00185 (Puna18p_00044) 31241..33535 - 2295 WP_336251289 DotA/TraY family protein -
MOP38_RS00190 (Puna18p_00043) 33450..34013 - 564 WP_241391500 conjugal transfer protein TraX -
MOP38_RS00195 (Puna18p_00042) 34006..35232 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOP38_RS00200 (Puna18p_00040) 35373..38447 - 3075 WP_241391501 type IV secretory system conjugative DNA transfer family protein traU
MOP38_RS00205 (Puna18p_00039) 38450..39055 - 606 WP_241391502 hypothetical protein traT
MOP38_RS00210 (Puna18p_00038) 39141..39542 - 402 WP_010895871 DUF6750 family protein traR
MOP38_RS00215 (Puna18p_00037) 39558..40088 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOP38_RS00220 (Puna18p_00036) 40097..40840 - 744 WP_241391503 hypothetical protein traP
MOP38_RS00225 (Puna18p_00035) 40840..42072 - 1233 WP_241391504 conjugal transfer protein TraO traO
MOP38_RS00230 (Puna18p_00034) 42076..43083 - 1008 WP_241389930 DotH/IcmK family type IV secretion protein traN
MOP38_RS00235 (Puna18p_00033) 43086..43772 - 687 WP_241391505 DotI/IcmL/TraM family protein traM
MOP38_RS00240 (Puna18p_00032) 43851..44342 - 492 WP_241391506 hypothetical protein traL
MOP38_RS00245 (Puna18p_00031) 44352..47588 - 3237 WP_241391507 LPD7 domain-containing protein -
MOP38_RS00250 (Puna18p_00029) 47731..47997 - 267 WP_241390169 IcmT/TraK family protein traK
MOP38_RS00255 (Puna18p_00028) 47997..49154 - 1158 WP_241391508 plasmid transfer ATPase TraJ virB11
MOP38_RS00260 (Puna18p_00026) 49344..50132 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP38_RS00265 (Puna18p_00025) 50129..50587 - 459 WP_241389614 DotD/TraH family lipoprotein -
MOP38_RS00270 (Puna18p_00024) 50625..52058 - 1434 WP_241391509 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP38_RS00275 (Puna18p_00023) 52055..52723 - 669 WP_241391510 prepilin peptidase -
MOP38_RS00280 (Puna18p_00022) 52723..53208 - 486 WP_241391511 lytic transglycosylase domain-containing protein virB1
MOP38_RS00285 (Puna18p_00021) 53263..53853 - 591 WP_241391512 type 4 pilus major pilin -
MOP38_RS00290 (Puna18p_00020) 53888..55003 - 1116 WP_241391513 type II secretion system F family protein -
MOP38_RS00295 (Puna18p_00019) 55000..56490 - 1491 WP_241391514 ATPase, T2SS/T4P/T4SS family virB11
MOP38_RS00300 (Puna18p_00018) 56496..57074 - 579 WP_241391515 type IV pilus biogenesis protein PilP -
MOP38_RS00305 (Puna18p_00017) 57061..58371 - 1311 WP_241391516 type 4b pilus protein PilO2 -
MOP38_RS00310 (Puna18p_00016) 58375..60009 - 1635 WP_241391517 PilN family type IVB pilus formation outer membrane protein -
MOP38_RS00315 (Puna18p_00015) 60033..60458 - 426 WP_241389892 type IV pilus biogenesis protein PilM -
MOP38_RS00320 (Puna18p_00014) 60458..61402 - 945 WP_241391518 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3915 GenBank   NZ_MT039215
Plasmid name   AGR_Puna18|unnamed1 Incompatibility group   -
Plasmid size   176312 bp Coordinate of oriT [Strand]   22671..22753 [+]
Host baterium   Serratia proteamaculans strain AGR_Puna18

Cargo genes

Drug resistance gene   -
Virulence gene   pilW
Metal resistance gene   -
Degradation gene   bphB
Symbiosis gene   nifH, nifD
Anti-CRISPR   AcrIIC1