Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103470
Name   oriT_AGR_336|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_336
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039188 (22608..22690 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_336|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAAGGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2593 GenBank   WP_241390305
Name   Relaxase_MOP14_RS00150_AGR_336|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73396.69 Da        Isoelectric Point: 7.3282

>WP_241390305.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVDLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKVADDCFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVSECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKCPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRPGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2393 GenBank   WP_241390158
Name   trbC_MOP14_RS00190_AGR_336|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81527.35 Da        Isoelectric Point: 8.3675

>WP_241390158.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33487..62978

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP14_RS00180 (336p_00040) 29696..30640 + 945 WP_241389593 hypothetical protein -
MOP14_RS00185 (336p_00041) 30776..31261 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOP14_RS00190 (336p_00042) 31272..33449 - 2178 WP_241390158 F-type conjugative transfer protein TrbC -
MOP14_RS00195 (336p_00043) 33487..34485 - 999 WP_241390308 hypothetical protein trbB
MOP14_RS00200 (336p_00044) 34667..35707 - 1041 WP_241390309 IS481 family transposase -
MOP14_RS00720 (336p_00045) 35802..36056 - 255 WP_241391204 hypothetical protein trbA
MOP14_RS00210 (336p_00047) 37149..37727 - 579 WP_241389923 hypothetical protein -
MOP14_RS00215 (336p_00048) 37724..40018 - 2295 WP_261097221 DotA/TraY family protein -
MOP14_RS00220 (336p_00049) 39933..40496 - 564 WP_241390311 conjugal transfer protein TraX -
MOP14_RS00225 (336p_00050) 40489..41715 - 1227 WP_241390312 conjugal transfer protein TraW traW
MOP14_RS00230 (336p_00052) 41856..44930 - 3075 WP_241390313 type IV secretory system conjugative DNA transfer family protein traU
MOP14_RS00235 (336p_00053) 44933..45538 - 606 WP_241389875 hypothetical protein traT
MOP14_RS00240 (336p_00054) 45625..46026 - 402 WP_241389604 DUF6750 family protein traR
MOP14_RS00245 (336p_00055) 46042..46572 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOP14_RS00250 (336p_00056) 46581..47324 - 744 WP_241390314 hypothetical protein traP
MOP14_RS00255 (336p_00057) 47324..48556 - 1233 WP_241390315 conjugal transfer protein TraO traO
MOP14_RS00260 (336p_00058) 48560..49567 - 1008 WP_241390316 DotH/IcmK family type IV secretion protein traN
MOP14_RS00265 (336p_00059) 49570..50244 - 675 WP_241390317 DotI/IcmL/TraM family protein traM
MOP14_RS00270 (336p_00060) 50335..50826 - 492 WP_010895743 hypothetical protein traL
MOP14_RS00275 (336p_00061) 50836..54072 - 3237 WP_241390318 LPD7 domain-containing protein -
MOP14_RS00280 (336p_00063) 54215..54481 - 267 WP_241390319 IcmT/TraK family protein traK
MOP14_RS00285 (336p_00064) 54481..55638 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOP14_RS00290 (336p_00066) 55828..56616 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP14_RS00295 (336p_00067) 56613..57071 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP14_RS00300 (336p_00068) 57109..58542 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP14_RS00305 (336p_00069) 58539..59207 - 669 WP_241390170 prepilin peptidase -
MOP14_RS00310 (336p_00070) 59207..59692 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP14_RS00315 (336p_00071) 59747..60340 - 594 WP_010895752 type 4 pilus major pilin -
MOP14_RS00320 (336p_00072) 60376..61488 - 1113 WP_241389818 type II secretion system F family protein -
MOP14_RS00325 (336p_00073) 61488..62978 - 1491 WP_241389888 ATPase, T2SS/T4P/T4SS family virB11
MOP14_RS00330 (336p_00074) 62984..63568 - 585 WP_241389889 type IV pilus biogenesis protein PilP -
MOP14_RS00335 (336p_00075) 63555..64865 - 1311 WP_241390279 type 4b pilus protein PilO2 -
MOP14_RS00340 (336p_00076) 64869..66503 - 1635 WP_241389891 PilN family type IVB pilus formation outer membrane protein -
MOP14_RS00345 (336p_00077) 66525..66950 - 426 WP_241389892 type IV pilus biogenesis protein PilM -
MOP14_RS00350 (336p_00078) 66950..67963 - 1014 WP_241390280 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3913 GenBank   NZ_MT039188
Plasmid name   AGR_336|unnamed1 Incompatibility group   -
Plasmid size   147897 bp Coordinate of oriT [Strand]   22608..22690 [+]
Host baterium   Serratia proteamaculans strain AGR_336

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -