Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103467
Name   oriT_AGR_25E|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_25E
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039178 (22307..22389 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_25E|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCTGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2590 GenBank   WP_241390259
Name   Relaxase_MOO83_RS00135_AGR_25E|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73466.71 Da        Isoelectric Point: 7.4877

>WP_241390259.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPHGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKSRYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKL
SAVEQPEVEQNKLRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2390 GenBank   WP_241390261
Name   trbC_MOO83_RS00150_AGR_25E|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81485.25 Da        Isoelectric Point: 8.1675

>WP_241390261.1 F-type conjugative transfer protein TrbC [Serratia proteamaculans]
MSRPVEVDPRRVRRPLGYTFINDALLSPIGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
IFQQLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27853..56144

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO83_RS00140 (25Ep1_00033) 24758..25081 - 324 WP_010895860 hypothetical protein -
MOO83_RS00145 (25Ep1_00034) 25091..25615 - 525 WP_241390260 phospholipase D family protein -
MOO83_RS00150 (25Ep1_00035) 25638..27815 - 2178 WP_241390261 F-type conjugative transfer protein TrbC -
MOO83_RS00155 (25Ep1_00036) 27853..28866 - 1014 WP_241390262 hypothetical protein trbB
MOO83_RS00160 (25Ep1_00037) 28880..30118 - 1239 WP_241390263 conjugal transfer protein TrbA trbA
MOO83_RS00165 (25Ep1_00038) 30319..30897 - 579 WP_241390264 hypothetical protein -
MOO83_RS00170 (25Ep1_00039) 30894..33188 - 2295 WP_336251218 DotA/TraY family protein -
MOO83_RS00175 (25Ep1_00040) 33103..33666 - 564 WP_241390265 conjugal transfer protein TraX -
MOO83_RS00180 (25Ep1_00041) 33659..34885 - 1227 WP_241390266 conjugal transfer protein TraW traW
MOO83_RS00185 (25Ep1_00043) 35026..38100 - 3075 WP_241390267 type IV secretory system conjugative DNA transfer family protein traU
MOO83_RS00190 (25Ep1_00044) 38103..38708 - 606 WP_010895870 TraT traT
MOO83_RS00195 (25Ep1_00045) 38794..39195 - 402 WP_017891138 DUF6750 family protein traR
MOO83_RS00200 (25Ep1_00046) 39211..39741 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO83_RS00205 (25Ep1_00047) 39750..40493 - 744 WP_010895873 hypothetical protein traP
MOO83_RS00210 (25Ep1_00048) 40493..41725 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOO83_RS00215 (25Ep1_00049) 41730..42737 - 1008 WP_241390268 DotH/IcmK family type IV secretion protein traN
MOO83_RS00220 (25Ep1_00050) 42740..43414 - 675 WP_241390269 DotI/IcmL/TraM family protein traM
MOO83_RS00225 (25Ep1_00051) 43505..43996 - 492 WP_241390270 hypothetical protein traL
MOO83_RS00230 (25Ep1_00052) 44006..47239 - 3234 WP_241390271 LPD7 domain-containing protein -
MOO83_RS00235 (25Ep1_00054) 47382..47648 - 267 WP_241390169 IcmT/TraK family protein traK
MOO83_RS00240 (25Ep1_00055) 47648..48805 - 1158 WP_241390272 plasmid transfer ATPase TraJ virB11
MOO83_RS00245 (25Ep1_00057) 48995..49783 - 789 WP_241390273 type IV secretory system conjugative DNA transfer family protein traI
MOO83_RS00250 (25Ep1_00058) 49780..50238 - 459 WP_241390274 DotD/TraH family lipoprotein -
MOO83_RS00255 (25Ep1_00059) 50275..51708 - 1434 WP_241390275 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO83_RS00260 (25Ep1_00060) 51705..52373 - 669 WP_241390232 prepilin peptidase -
MOO83_RS00265 (25Ep1_00061) 52373..52858 - 486 WP_241390233 lytic transglycosylase domain-containing protein virB1
MOO83_RS00270 (25Ep1_00062) 52913..53506 - 594 WP_241390234 type 4 pilus major pilin -
MOO83_RS00275 (25Ep1_00063) 53542..54594 - 1053 WP_241390235 type II secretion system F family protein -
MOO83_RS00280 (25Ep1_00064) 54654..56144 - 1491 WP_241390236 ATPase, T2SS/T4P/T4SS family virB11
MOO83_RS00285 (25Ep1_00065) 56150..56734 - 585 WP_241390237 type IV pilus biogenesis protein PilP -
MOO83_RS00290 (25Ep1_00066) 56721..58016 - 1296 WP_241390238 type 4b pilus protein PilO2 -
MOO83_RS00295 (25Ep1_00067) 58020..59654 - 1635 WP_241390239 PilN family type IVB pilus formation outer membrane protein -
MOO83_RS00300 (25Ep1_00068) 59679..60104 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOO83_RS00305 (25Ep1_00069) 60104..61048 - 945 WP_241390240 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3910 GenBank   NZ_MT039178
Plasmid name   AGR_25E|unnamed1 Incompatibility group   -
Plasmid size   103391 bp Coordinate of oriT [Strand]   22307..22389 [+]
Host baterium   Serratia proteamaculans strain AGR_25E

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -