Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103466
Name   oriT_AGR_K|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_K
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039209 (22613..22695 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_K|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAAGGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2589 GenBank   WP_241390305
Name   Relaxase_MOP08_RS00145_AGR_K|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73396.69 Da        Isoelectric Point: 7.3282

>WP_241390305.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVDLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKVADDCFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVSECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKCPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRPGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2389 GenBank   WP_241390158
Name   trbC_MOP08_RS00185_AGR_K|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81527.35 Da        Isoelectric Point: 8.3675

>WP_241390158.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33492..61771

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP08_RS00175 (Kp_00036) 29701..30645 + 945 WP_241389593 hypothetical protein -
MOP08_RS00180 (Kp_00037) 30781..31266 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOP08_RS00185 (Kp_00038) 31277..33454 - 2178 WP_241390158 F-type conjugative transfer protein TrbC -
MOP08_RS00190 (Kp_00039) 33492..34490 - 999 WP_241390308 hypothetical protein trbB
MOP08_RS00195 (Kp_00040) 34503..35813 - 1311 WP_241390430 conjugal transfer protein TrbA trbA
MOP08_RS00200 (Kp_00041) 35942..36520 - 579 WP_241389923 hypothetical protein -
MOP08_RS00205 (Kp_00042) 36517..38811 - 2295 WP_261097221 DotA/TraY family protein -
MOP08_RS00210 (Kp_00043) 38726..39289 - 564 WP_241390311 conjugal transfer protein TraX -
MOP08_RS00215 (Kp_00044) 39282..40508 - 1227 WP_241390312 conjugal transfer protein TraW traW
MOP08_RS00220 (Kp_00046) 40649..43723 - 3075 WP_241390313 type IV secretory system conjugative DNA transfer family protein traU
MOP08_RS00225 (Kp_00047) 43726..44331 - 606 WP_241389875 hypothetical protein traT
MOP08_RS00230 (Kp_00048) 44418..44819 - 402 WP_241389604 DUF6750 family protein traR
MOP08_RS00235 (Kp_00049) 44835..45365 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOP08_RS00240 (Kp_00050) 45374..46117 - 744 WP_241390314 hypothetical protein traP
MOP08_RS00245 (Kp_00051) 46117..47349 - 1233 WP_241389929 conjugal transfer protein TraO traO
MOP08_RS00250 (Kp_00052) 47353..48360 - 1008 WP_241390316 DotH/IcmK family type IV secretion protein traN
MOP08_RS00255 (Kp_00053) 48363..49037 - 675 WP_241390317 DotI/IcmL/TraM family protein traM
MOP08_RS00260 (Kp_00054) 49128..49619 - 492 WP_010895743 hypothetical protein traL
MOP08_RS00265 (Kp_00055) 49629..52865 - 3237 WP_241390318 LPD7 domain-containing protein -
MOP08_RS00270 (Kp_00057) 53008..53274 - 267 WP_241390319 IcmT/TraK family protein traK
MOP08_RS00275 (Kp_00058) 53274..54431 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOP08_RS00280 (Kp_00060) 54621..55409 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP08_RS00285 (Kp_00061) 55406..55864 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP08_RS00290 (Kp_00062) 55902..57335 - 1434 WP_241390921 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP08_RS00295 (Kp_00063) 57332..58000 - 669 WP_241390170 prepilin peptidase -
MOP08_RS00300 (Kp_00064) 58000..58485 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP08_RS00305 (Kp_00065) 58540..59133 - 594 WP_010895752 type 4 pilus major pilin -
MOP08_RS00310 (Kp_00066) 59169..60281 - 1113 WP_241389818 type II secretion system F family protein -
MOP08_RS00315 (Kp_00067) 60281..61771 - 1491 WP_241389888 ATPase, T2SS/T4P/T4SS family virB11
MOP08_RS00320 (Kp_00068) 61777..62361 - 585 WP_241389889 type IV pilus biogenesis protein PilP -
MOP08_RS00325 (Kp_00069) 62348..63658 - 1311 WP_241390279 type 4b pilus protein PilO2 -
MOP08_RS00330 (Kp_00070) 63662..65296 - 1635 WP_241389891 PilN family type IVB pilus formation outer membrane protein -
MOP08_RS00335 (Kp_00071) 65318..65743 - 426 WP_241389892 type IV pilus biogenesis protein PilM -
MOP08_RS00340 (Kp_00072) 65743..66756 - 1014 WP_241390280 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3909 GenBank   NZ_MT039209
Plasmid name   AGR_K|unnamed1 Incompatibility group   -
Plasmid size   106530 bp Coordinate of oriT [Strand]   22613..22695 [+]
Host baterium   Serratia proteamaculans strain AGR_K

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -