Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103463 |
| Name | oriT_AGR_F28|unnamed1  |
| Organism | Serratia proteamaculans strain AGR_F28 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT039207 (70458..70539 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_AGR_F28|unnamed1
CGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC
CGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2586 | GenBank | WP_241390631 |
| Name | Relaxase_MOP05_RS00375_AGR_F28|unnamed1 |
UniProt ID | _ |
| Length | 651 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 651 a.a. Molecular weight: 73670.85 Da Isoelectric Point: 6.9859
>WP_241390631.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDTPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPHGHTQVMFDGVMCETNCFNITTASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKSLTPSGAKALEYYADVESLHTYAVTECGFKIDEAMADPNLTWKDVHRILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLGCLEKDLGLFQPLENVGTYTKDYTTTATDAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQTYKHAWKRPKLDANVVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLAVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPISTNSIVFAVADDTPAFALDGYATQVTRDGTVQYMHDGCVELQDKGERIEVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGESDFVQQACSMVRWFNEGGEEPLPLSDPQQRMMAGYDAYSQTISTGDGQHTP
SAAEQPNVEQSKPRSGYRPQQ
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDTPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPHGHTQVMFDGVMCETNCFNITTASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKSLTPSGAKALEYYADVESLHTYAVTECGFKIDEAMADPNLTWKDVHRILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLGCLEKDLGLFQPLENVGTYTKDYTTTATDAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQTYKHAWKRPKLDANVVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLAVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPISTNSIVFAVADDTPAFALDGYATQVTRDGTVQYMHDGCVELQDKGERIEVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGESDFVQQACSMVRWFNEGGEEPLPLSDPQQRMMAGYDAYSQTISTGDGQHTP
SAAEQPNVEQSKPRSGYRPQQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2385 | GenBank | WP_241390633 |
| Name | trbC_MOP05_RS00390_AGR_F28|unnamed1 |
UniProt ID | _ |
| Length | 725 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 725 a.a. Molecular weight: 81741.52 Da Isoelectric Point: 8.3731
>WP_241390633.1 F-type conjugative transfer protein TrbC [Serratia proteamaculans]
MTRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIVGFIMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRHPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELINKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPITMDELGAYFGQGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMASSLPMRINRFIDVETPTEAELFTLNPSLKRKLPPAAQEIDG
ILQRLEQAMDTTTSAGMMDPVLKRVAAVALDLDNRADVSYSPTQRGVLLFEAAREALHQNKRNWRHLPSP
PKPIRVSKEVAQSLANAGTNQITFR
MTRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIVGFIMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRHPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELINKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPITMDELGAYFGQGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMASSLPMRINRFIDVETPTEAELFTLNPSLKRKLPPAAQEIDG
ILQRLEQAMDTTTSAGMMDPVLKRVAAVALDLDNRADVSYSPTQRGVLLFEAAREALHQNKRNWRHLPSP
PKPIRVSKEVAQSLANAGTNQITFR
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2215..24588
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MOP05_RS00010 (F28p_00001) | 1659..2222 | - | 564 | WP_241390638 | conjugal transfer protein TraX | - |
| MOP05_RS00015 (F28p_00002) | 2215..3441 | - | 1227 | WP_241390639 | conjugal transfer protein TraW | traW |
| MOP05_RS00020 (F28p_00004) | 3582..6656 | - | 3075 | WP_241390640 | type IV secretory system conjugative DNA transfer family protein | traU |
| MOP05_RS00025 (F28p_00005) | 6659..7264 | - | 606 | WP_241390641 | hypothetical protein | traT |
| MOP05_RS00030 (F28p_00006) | 7349..7750 | - | 402 | WP_241390642 | DUF6750 family protein | traR |
| MOP05_RS00035 (F28p_00007) | 7766..8296 | - | 531 | WP_010895872 | conjugal transfer protein TraQ | traQ |
| MOP05_RS00040 (F28p_00008) | 8305..9048 | - | 744 | WP_241390643 | hypothetical protein | traP |
| MOP05_RS00045 (F28p_00009) | 9048..10280 | - | 1233 | WP_241390644 | conjugal transfer protein TraO | traO |
| MOP05_RS00050 (F28p_00010) | 10285..11292 | - | 1008 | WP_241390645 | DotH/IcmK family type IV secretion protein | traN |
| MOP05_RS00055 (F28p_00011) | 11295..11969 | - | 675 | WP_241390646 | DotI/IcmL/TraM family protein | traM |
| MOP05_RS00060 (F28p_00012) | 12060..12551 | - | 492 | WP_241390647 | hypothetical protein | traL |
| MOP05_RS00065 (F28p_00013) | 12561..15794 | - | 3234 | WP_241390648 | LPD7 domain-containing protein | - |
| MOP05_RS00070 (F28p_00015) | 15937..16203 | - | 267 | WP_241390675 | IcmT/TraK family protein | traK |
| MOP05_RS00075 (F28p_00016) | 16254..17411 | - | 1158 | WP_115185023 | plasmid transfer ATPase TraJ | virB11 |
| MOP05_RS00080 (F28p_00017) | 17442..18230 | - | 789 | WP_241390649 | type IV secretory system conjugative DNA transfer family protein | traI |
| MOP05_RS00085 (F28p_00018) | 18227..18652 | - | 426 | WP_241390650 | DotD/TraH family lipoprotein | - |
| MOP05_RS00090 (F28p_00019) | 18722..20188 | - | 1467 | WP_241390651 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| MOP05_RS00095 (F28p_00020) | 20152..20814 | - | 663 | WP_241390652 | A24 family peptidase | - |
| MOP05_RS00100 (F28p_00021) | 20820..21305 | - | 486 | WP_241390653 | lytic transglycosylase domain-containing protein | virB1 |
| MOP05_RS00105 (F28p_00022) | 21360..21950 | - | 591 | WP_115185017 | type 4 pilus major pilin | - |
| MOP05_RS00110 (F28p_00023) | 21986..23101 | - | 1116 | WP_241390654 | type II secretion system F family protein | - |
| MOP05_RS00115 (F28p_00024) | 23098..24588 | - | 1491 | WP_241390655 | ATPase, T2SS/T4P/T4SS family | virB11 |
| MOP05_RS00120 (F28p_00025) | 24594..25178 | - | 585 | WP_241390656 | type IV pilus biogenesis protein PilP | - |
| MOP05_RS00125 (F28p_00026) | 25165..26367 | - | 1203 | WP_241390657 | type 4b pilus protein PilO2 | - |
| MOP05_RS00130 (F28p_00027) | 26473..28107 | - | 1635 | WP_241390658 | PilN family type IVB pilus formation outer membrane protein | - |
| MOP05_RS00135 (F28p_00028) | 28130..28555 | - | 426 | WP_017891030 | type IV pilus biogenesis protein PilM | - |
| MOP05_RS00140 (F28p_00029) | 28555..29580 | - | 1026 | WP_241390661 | toxin co-regulated pilus biosynthesis Q family protein | - |
Region 2: 76006..104183
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MOP05_RS00380 (F28p_00076) | 72911..73234 | - | 324 | WP_129935345 | hypothetical protein | - |
| MOP05_RS00385 (F28p_00077) | 73244..73780 | - | 537 | WP_241390632 | phospholipase D family protein | - |
| MOP05_RS00390 (F28p_00078) | 73791..75968 | - | 2178 | WP_241390633 | F-type conjugative transfer protein TrbC | - |
| MOP05_RS00395 (F28p_00079) | 76006..77019 | - | 1014 | WP_241390634 | hypothetical protein | trbB |
| MOP05_RS00400 (F28p_00080) | 77033..78292 | - | 1260 | WP_241390635 | conjugal transfer protein TrbA | trbA |
| MOP05_RS00405 (F28p_00081) | 78470..79048 | - | 579 | WP_241390636 | hypothetical protein | - |
| MOP05_RS00410 (F28p_00082) | 79045..81189 | - | 2145 | WP_241390637 | DotA/TraY family protein | traY |
| MOP05_RS00415 (F28p_00083) | 81254..81817 | - | 564 | WP_241390638 | conjugal transfer protein TraX | - |
| MOP05_RS00420 (F28p_00084) | 81810..83036 | - | 1227 | WP_241390639 | conjugal transfer protein TraW | traW |
| MOP05_RS00425 (F28p_00086) | 83177..86251 | - | 3075 | WP_241390640 | type IV secretory system conjugative DNA transfer family protein | traU |
| MOP05_RS00430 (F28p_00087) | 86254..86859 | - | 606 | WP_241390641 | hypothetical protein | traT |
| MOP05_RS00435 (F28p_00088) | 86944..87345 | - | 402 | WP_241390642 | DUF6750 family protein | traR |
| MOP05_RS00440 (F28p_00089) | 87361..87891 | - | 531 | WP_010895872 | conjugal transfer protein TraQ | traQ |
| MOP05_RS00445 (F28p_00090) | 87900..88643 | - | 744 | WP_241390643 | hypothetical protein | traP |
| MOP05_RS00450 (F28p_00091) | 88643..89875 | - | 1233 | WP_241390644 | conjugal transfer protein TraO | traO |
| MOP05_RS00455 (F28p_00092) | 89880..90887 | - | 1008 | WP_241390645 | DotH/IcmK family type IV secretion protein | traN |
| MOP05_RS00460 (F28p_00093) | 90890..91564 | - | 675 | WP_241390646 | DotI/IcmL/TraM family protein | traM |
| MOP05_RS00465 (F28p_00094) | 91655..92146 | - | 492 | WP_241390647 | hypothetical protein | traL |
| MOP05_RS00470 (F28p_00095) | 92156..95389 | - | 3234 | WP_241390648 | LPD7 domain-containing protein | - |
| MOP05_RS00475 (F28p_00097) | 95532..95798 | - | 267 | WP_241390675 | IcmT/TraK family protein | traK |
| MOP05_RS00480 (F28p_00098) | 95849..97006 | - | 1158 | WP_115185023 | plasmid transfer ATPase TraJ | virB11 |
| MOP05_RS00485 (F28p_00099) | 97037..97825 | - | 789 | WP_241390649 | type IV secretory system conjugative DNA transfer family protein | traI |
| MOP05_RS00490 (F28p_00100) | 97822..98247 | - | 426 | WP_241390650 | DotD/TraH family lipoprotein | - |
| MOP05_RS00495 (F28p_00101) | 98317..99783 | - | 1467 | WP_241390651 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| MOP05_RS00500 (F28p_00102) | 99747..100409 | - | 663 | WP_241390652 | A24 family peptidase | - |
| MOP05_RS00505 (F28p_00103) | 100415..100900 | - | 486 | WP_241390653 | lytic transglycosylase domain-containing protein | virB1 |
| MOP05_RS00510 (F28p_00104) | 100955..101545 | - | 591 | WP_115185017 | type 4 pilus major pilin | - |
| MOP05_RS00515 (F28p_00105) | 101581..102696 | - | 1116 | WP_241390654 | type II secretion system F family protein | - |
| MOP05_RS00520 (F28p_00106) | 102693..104183 | - | 1491 | WP_241390655 | ATPase, T2SS/T4P/T4SS family | virB11 |
| MOP05_RS00525 (F28p_00107) | 104189..104773 | - | 585 | WP_241390656 | type IV pilus biogenesis protein PilP | - |
| MOP05_RS00530 (F28p_00108) | 104760..105962 | - | 1203 | WP_241390657 | type 4b pilus protein PilO2 | - |
| MOP05_RS00535 (F28p_00109) | 106068..107702 | - | 1635 | WP_241390658 | PilN family type IVB pilus formation outer membrane protein | - |
| MOP05_RS00540 (F28p_00110) | 107725..108150 | - | 426 | WP_017891030 | type IV pilus biogenesis protein PilM | - |
| MOP05_RS00545 (F28p_00111) | 108150..108455 | - | 306 | WP_241390659 | toxin co-regulated pilus biosynthesis Q family protein | - |
Host bacterium
| ID | 3906 | GenBank | NZ_MT039207 |
| Plasmid name | AGR_F28|unnamed1 | Incompatibility group | - |
| Plasmid size | 108588 bp | Coordinate of oriT [Strand] | 70458..70539 [+] |
| Host baterium | Serratia proteamaculans strain AGR_F28 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |