Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103458
Name   oriT_AGR_1071|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_1071
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039194 (21269..21350 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_AGR_1071|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGTGCCCCGACACCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2582 GenBank   WP_241389921
Name   Relaxase_MOP09_RS00130_AGR_1071|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73479.70 Da        Isoelectric Point: 7.4607

>WP_241389921.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDDDKPDTPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVGLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKEKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
SGMALAENKSGEYLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)


  Protein structure



No available structure.




T4CP

ID   2379 GenBank   WP_241389595
Name   trbC_MOP09_RS00170_AGR_1071|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81541.37 Da        Isoelectric Point: 8.3675

>WP_241389595.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDILHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32146..60452

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP09_RS00160 (1071p1_00040) 28355..29299 + 945 WP_241389593 hypothetical protein -
MOP09_RS00165 (1071p1_00041) 29435..29920 - 486 WP_241389594 phospholipase D-like domain-containing protein -
MOP09_RS00170 (1071p1_00042) 29931..32108 - 2178 WP_241389595 F-type conjugative transfer protein TrbC -
MOP09_RS00175 (1071p1_00043) 32146..33144 - 999 WP_241389596 hypothetical protein trbB
MOP09_RS00180 (1071p1_00044) 33156..34466 - 1311 WP_241389597 conjugal transfer protein TrbA trbA
MOP09_RS00185 (1071p1_00045) 34605..35186 - 582 WP_241390358 hypothetical protein -
MOP09_RS00190 (1071p1_00046) 35183..37327 - 2145 WP_241389599 DotA/TraY family protein traY
MOP09_RS00195 (1071p1_00047) 37392..37964 - 573 WP_241390359 conjugal transfer protein TraX -
MOP09_RS00200 (1071p1_00048) 37957..39183 - 1227 WP_241389601 conjugal transfer protein TraW traW
MOP09_RS00205 (1071p1_00050) 39324..42398 - 3075 WP_241389602 type IV secretory system conjugative DNA transfer family protein traU
MOP09_RS00210 (1071p1_00051) 42401..43006 - 606 WP_241390360 hypothetical protein traT
MOP09_RS00215 (1071p1_00052) 43093..43494 - 402 WP_241389604 DUF6750 family protein traR
MOP09_RS00220 (1071p1_00053) 43510..44040 - 531 WP_241389605 conjugal transfer protein TraQ traQ
MOP09_RS00225 (1071p1_00054) 44049..44798 - 750 WP_241389606 hypothetical protein traP
MOP09_RS00230 (1071p1_00055) 44798..46030 - 1233 WP_241389607 conjugal transfer protein TraO traO
MOP09_RS00235 (1071p1_00056) 46034..47041 - 1008 WP_241389608 DotH/IcmK family type IV secretion protein traN
MOP09_RS00240 (1071p1_00057) 47044..47718 - 675 WP_010895742 DotI/IcmL/TraM family protein traM
MOP09_RS00245 (1071p1_00058) 47809..48300 - 492 WP_241389610 hypothetical protein traL
MOP09_RS00250 (1071p1_00059) 48310..51546 - 3237 WP_241391250 LPD7 domain-containing protein -
MOP09_RS00255 (1071p1_00061) 51689..51955 - 267 WP_241389612 IcmT/TraK family protein traK
MOP09_RS00260 (1071p1_00062) 51955..53112 - 1158 WP_241389613 plasmid transfer ATPase TraJ virB11
MOP09_RS00265 (1071p1_00063) 53302..54090 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP09_RS00270 (1071p1_00064) 54087..54545 - 459 WP_241389614 DotD/TraH family lipoprotein -
MOP09_RS00275 (1071p1_00065) 54583..56016 - 1434 WP_241389615 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP09_RS00280 (1071p1_00066) 56013..56681 - 669 WP_241391248 prepilin peptidase -
MOP09_RS00285 (1071p1_00067) 56681..57166 - 486 WP_241389617 lytic transglycosylase domain-containing protein virB1
MOP09_RS00290 (1071p1_00068) 57221..57814 - 594 WP_010895752 type 4 pilus major pilin -
MOP09_RS00295 (1071p1_00069) 57850..58902 - 1053 WP_241390342 type II secretion system F family protein -
MOP09_RS00300 (1071p1_00070) 58962..60452 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOP09_RS00305 (1071p1_00071) 60458..61042 - 585 WP_241390343 type IV pilus biogenesis protein PilP -
MOP09_RS00310 (1071p1_00072) 61029..62339 - 1311 WP_241389532 type 4b pilus protein PilO2 -
MOP09_RS00315 (1071p1_00073) 62343..63977 - 1635 WP_241389533 PilN family type IVB pilus formation outer membrane protein -
MOP09_RS00320 (1071p1_00074) 64002..64427 - 426 WP_241390345 type IV pilus biogenesis protein PilM -
MOP09_RS00325 (1071p1_00075) 64427..65440 - 1014 WP_241390346 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3901 GenBank   NZ_MT039194
Plasmid name   AGR_1071|unnamed1 Incompatibility group   -
Plasmid size   108958 bp Coordinate of oriT [Strand]   21269..21350 [+]
Host baterium   Serratia proteamaculans strain AGR_1071

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -