Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103454
Name   oriT_AGR_1137|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_1137
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039196 (22252..22334 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_1137|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGAACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2578 GenBank   WP_241391119
Name   Relaxase_MOP17_RS00145_AGR_1137|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73415.61 Da        Isoelectric Point: 7.3518

>WP_241391119.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPHGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFESVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNDQGEIVRSKREKSLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHRILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLAVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALDGYATQVTRDGTVQYVRDGRVELQDKGERIEVGDHRVNEGEHIA
GGMALSENKSGEYLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRMMAGYESAYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2375 GenBank   WP_241391122
Name   trbC_MOP17_RS00160_AGR_1137|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81555.40 Da        Isoelectric Point: 7.9244

>WP_241391122.1 F-type conjugative transfer protein TrbC [Serratia proteamaculans]
MSRPVEVDPRRVRRPLGYTFINDALLSPIGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSSLPMRINRFIDVETPTEAELFALNPSLQLKLPPTAQEIDG
ILQRLDLALDITAPVGMMDPVLKRVAAVALDLDNRADVSYSPTQRGVLLFEAAREALRQNKRNWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27798..55936

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP17_RS00150 (1137p_00032) 24703..25026 - 324 WP_241391120 hypothetical protein -
MOP17_RS00155 (1137p_00033) 25036..25572 - 537 WP_241391121 phospholipase D family protein -
MOP17_RS00160 (1137p_00034) 25583..27760 - 2178 WP_241391122 F-type conjugative transfer protein TrbC -
MOP17_RS00165 (1137p_00035) 27798..28808 - 1011 WP_241391123 hypothetical protein trbB
MOP17_RS00170 (1137p_00036) 28820..30130 - 1311 WP_241391124 conjugal transfer protein TrbA trbA
MOP17_RS00175 (1137p_00037) 30269..30811 - 543 WP_241391125 hypothetical protein -
MOP17_RS00180 (1137p_00038) 30847..32991 - 2145 WP_241391126 DotA/TraY family protein traY
MOP17_RS00185 (1137p_00039) 33055..33618 - 564 WP_241391127 conjugal transfer protein TraX -
MOP17_RS00190 (1137p_00040) 33611..34837 - 1227 WP_241391128 conjugal transfer protein TraW traW
MOP17_RS00195 (1137p_00041) 34978..38052 - 3075 WP_241391129 type IV secretory system conjugative DNA transfer family protein traU
MOP17_RS00200 (1137p_00042) 38055..38660 - 606 WP_241391130 hypothetical protein traT
MOP17_RS00205 (1137p_00043) 38748..39149 - 402 WP_241391131 DUF6750 family protein traR
MOP17_RS00210 (1137p_00044) 39165..39695 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOP17_RS00215 (1137p_00045) 39704..40447 - 744 WP_241391132 hypothetical protein traP
MOP17_RS00220 (1137p_00046) 40447..41679 - 1233 WP_241391133 conjugal transfer protein TraO traO
MOP17_RS00225 (1137p_00047) 41684..42691 - 1008 WP_010895741 DotH/IcmK family type IV secretion protein traN
MOP17_RS00230 (1137p_00048) 42694..43368 - 675 WP_241391134 DotI/IcmL/TraM family protein traM
MOP17_RS00235 (1137p_00049) 43459..43950 - 492 WP_241391135 hypothetical protein traL
MOP17_RS00240 (1137p_00050) 43960..47193 - 3234 WP_241391136 LPD7 domain-containing protein -
MOP17_RS00245 (1137p_00052) 47336..47602 - 267 WP_241391137 IcmT/TraK family protein traK
MOP17_RS00250 (1137p_00053) 47602..48759 - 1158 WP_241391138 plasmid transfer ATPase TraJ virB11
MOP17_RS00255 (1137p_00055) 48949..49737 - 789 WP_241391139 type IV secretory system conjugative DNA transfer family protein traI
MOP17_RS00260 (1137p_00056) 49734..50192 - 459 WP_241391140 DotD/TraH family lipoprotein -
MOP17_RS00265 (1137p_00057) 50220..51500 - 1281 WP_241391141 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP17_RS00270 (1137p_00058) 51497..52165 - 669 WP_241391142 prepilin peptidase -
MOP17_RS00275 (1137p_00059) 52165..52650 - 486 WP_128865598 lytic transglycosylase domain-containing protein virB1
MOP17_RS00280 (1137p_00060) 52705..53298 - 594 WP_241391143 type 4 pilus major pilin -
MOP17_RS00285 (1137p_00061) 53334..54446 - 1113 WP_241391144 type II secretion system F family protein -
MOP17_RS00290 (1137p_00062) 54446..55936 - 1491 WP_241391145 ATPase, T2SS/T4P/T4SS family virB11
MOP17_RS00295 (1137p_00063) 55942..56520 - 579 WP_241391146 type IV pilus biogenesis protein PilP -
MOP17_RS00300 (1137p_00064) 56507..57817 - 1311 WP_241391147 type 4b pilus protein PilO2 -
MOP17_RS00305 (1137p_00065) 57821..59455 - 1635 WP_241391148 PilN family type IVB pilus formation outer membrane protein -
MOP17_RS00310 (1137p_00066) 59480..59905 - 426 WP_241391149 type IV pilus biogenesis protein PilM -
MOP17_RS00315 (1137p_00067) 59905..60849 - 945 WP_241391150 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3897 GenBank   NZ_MT039196
Plasmid name   AGR_1137|unnamed1 Incompatibility group   -
Plasmid size   122573 bp Coordinate of oriT [Strand]   22252..22334 [+]
Host baterium   Serratia proteamaculans strain AGR_1137

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -