Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103453
Name   oriT_AGR_LC|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_LC
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039210 (21379..21460 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_AGR_LC|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2577 GenBank   WP_241390156
Name   Relaxase_MOP35_RS00135_AGR_LC|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73425.56 Da        Isoelectric Point: 7.4607

>WP_241390156.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDDDKPDTPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDAGLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKEKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LSRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
SGMALAENKSGEYLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2374 GenBank   WP_241391749
Name   trbC_MOP35_RS00175_AGR_LC|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81511.29 Da        Isoelectric Point: 8.4532

>WP_241391749.1 F-type conjugative transfer protein TrbC [Serratia proteamaculans]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSISWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32257..60545

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP35_RS00165 (LCp1_00064) 28466..29410 + 945 WP_241389593 hypothetical protein -
MOP35_RS00170 (LCp1_00063) 29546..30031 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOP35_RS00175 (LCp1_00062) 30042..32219 - 2178 WP_241391749 F-type conjugative transfer protein TrbC -
MOP35_RS00180 (LCp1_00061) 32257..33255 - 999 WP_241391750 hypothetical protein trbB
MOP35_RS00185 (LCp1_00060) 33267..34577 - 1311 WP_241390159 conjugal transfer protein TrbA trbA
MOP35_RS00190 (LCp1_00059) 34716..35258 - 543 WP_241390160 hypothetical protein -
MOP35_RS00195 (LCp1_00058) 35294..37588 - 2295 WP_241391751 DotA/TraY family protein traY
MOP35_RS00200 (LCp1_00057) 37503..38066 - 564 WP_241390162 conjugal transfer protein TraX -
MOP35_RS00205 (LCp1_00056) 38059..39285 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOP35_RS00210 (LCp1_00054) 39426..42500 - 3075 WP_241390163 type IV secretory system conjugative DNA transfer family protein traU
MOP35_RS00215 (LCp1_00053) 42503..43108 - 606 WP_241390164 hypothetical protein traT
MOP35_RS00220 (LCp1_00052) 43194..43595 - 402 WP_010895871 DUF6750 family protein traR
MOP35_RS00225 (LCp1_00051) 43611..44141 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOP35_RS00230 (LCp1_00050) 44150..44893 - 744 WP_241390165 hypothetical protein traP
MOP35_RS00235 (LCp1_00049) 44893..46125 - 1233 WP_241390166 conjugal transfer protein TraO traO
MOP35_RS00240 (LCp1_00048) 46130..47137 - 1008 WP_241390167 DotH/IcmK family type IV secretion protein traN
MOP35_RS00245 (LCp1_00047) 47140..47814 - 675 WP_010895742 DotI/IcmL/TraM family protein traM
MOP35_RS00250 (LCp1_00046) 47905..48396 - 492 WP_010895743 hypothetical protein traL
MOP35_RS00255 (LCp1_00045) 48406..51639 - 3234 WP_241390168 LPD7 domain-containing protein -
MOP35_RS00260 (LCp1_00043) 51782..52048 - 267 WP_241390169 IcmT/TraK family protein traK
MOP35_RS00265 (LCp1_00042) 52048..53205 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOP35_RS00270 (LCp1_00040) 53395..54183 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP35_RS00275 (LCp1_00039) 54180..54638 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP35_RS00280 (LCp1_00038) 54676..56109 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP35_RS00285 (LCp1_00037) 56106..56774 - 669 WP_241391752 prepilin peptidase -
MOP35_RS00290 (LCp1_00036) 56774..57259 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP35_RS00295 (LCp1_00035) 57314..57907 - 594 WP_010895752 type 4 pilus major pilin -
MOP35_RS00300 (LCp1_00034) 57943..59055 - 1113 WP_241389818 type II secretion system F family protein -
MOP35_RS00305 (LCp1_00033) 59055..60545 - 1491 WP_241390171 ATPase, T2SS/T4P/T4SS family virB11
MOP35_RS00310 (LCp1_00032) 60551..61135 - 585 WP_241389889 type IV pilus biogenesis protein PilP -
MOP35_RS00315 (LCp1_00031) 61122..62432 - 1311 WP_241389532 type 4b pilus protein PilO2 -
MOP35_RS00320 (LCp1_00030) 62436..64070 - 1635 WP_241390126 PilN family type IVB pilus formation outer membrane protein -
MOP35_RS00325 (LCp1_00029) 64095..64520 - 426 WP_010895758 type IV pilus biogenesis protein PilM -

Host bacterium

ID   3896 GenBank   NZ_MT039210
Plasmid name   AGR_LC|unnamed1 Incompatibility group   -
Plasmid size   120569 bp Coordinate of oriT [Strand]   21379..21460 [+]
Host baterium   Serratia proteamaculans strain AGR_LC

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -