Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103452
Name   oriT_AGR_465|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_465
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039189 (22640..22722 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_465|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAAGGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2576 GenBank   WP_241390305
Name   Relaxase_MOO99_RS00140_AGR_465|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73396.69 Da        Isoelectric Point: 7.3282

>WP_241390305.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVDLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKVADDCFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVSECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKCPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRPGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2373 GenBank   WP_241390158
Name   trbC_MOO99_RS00180_AGR_465|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81527.35 Da        Isoelectric Point: 8.3675

>WP_241390158.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33519..61798

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO99_RS00170 (465p1_00049) 29728..30672 + 945 WP_241389593 hypothetical protein -
MOO99_RS00175 (465p1_00050) 30808..31293 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOO99_RS00180 (465p1_00051) 31304..33481 - 2178 WP_241390158 F-type conjugative transfer protein TrbC -
MOO99_RS00185 (465p1_00052) 33519..34517 - 999 WP_241390308 hypothetical protein trbB
MOO99_RS00190 (465p1_00053) 34530..35753 - 1224 WP_241391019 conjugal transfer protein TrbA trbA
MOO99_RS00195 (465p1_00054) 35969..36547 - 579 WP_241389923 hypothetical protein -
MOO99_RS00200 (465p1_00055) 36544..38838 - 2295 WP_261097221 DotA/TraY family protein -
MOO99_RS00205 (465p1_00056) 38753..39316 - 564 WP_241390311 conjugal transfer protein TraX -
MOO99_RS00210 (465p1_00057) 39309..40535 - 1227 WP_241390312 conjugal transfer protein TraW traW
MOO99_RS00215 (465p1_00059) 40676..43750 - 3075 WP_241390313 type IV secretory system conjugative DNA transfer family protein traU
MOO99_RS00220 (465p1_00060) 43753..44358 - 606 WP_241389875 hypothetical protein traT
MOO99_RS00225 (465p1_00061) 44445..44846 - 402 WP_241389604 DUF6750 family protein traR
MOO99_RS00230 (465p1_00062) 44862..45392 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOO99_RS00235 (465p1_00063) 45401..46144 - 744 WP_241390314 hypothetical protein traP
MOO99_RS00240 (465p1_00064) 46144..47376 - 1233 WP_241389929 conjugal transfer protein TraO traO
MOO99_RS00245 (465p1_00065) 47380..48387 - 1008 WP_241390316 DotH/IcmK family type IV secretion protein traN
MOO99_RS00250 (465p1_00066) 48390..49064 - 675 WP_241390317 DotI/IcmL/TraM family protein traM
MOO99_RS00255 (465p1_00067) 49155..49646 - 492 WP_010895743 hypothetical protein traL
MOO99_RS00260 (465p1_00068) 49656..52892 - 3237 WP_241390318 LPD7 domain-containing protein -
MOO99_RS00265 (465p1_00070) 53035..53301 - 267 WP_241390319 IcmT/TraK family protein traK
MOO99_RS00270 (465p1_00071) 53301..54458 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOO99_RS00275 (465p1_00073) 54648..55436 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO99_RS00280 (465p1_00074) 55433..55891 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOO99_RS00285 (465p1_00075) 55929..57362 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO99_RS00290 (465p1_00076) 57359..58027 - 669 WP_241390170 prepilin peptidase -
MOO99_RS00295 (465p1_00077) 58027..58512 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOO99_RS00300 (465p1_00078) 58567..59160 - 594 WP_010895752 type 4 pilus major pilin -
MOO99_RS00305 (465p1_00079) 59196..60308 - 1113 WP_241389818 type II secretion system F family protein -
MOO99_RS00310 (465p1_00080) 60308..61798 - 1491 WP_241389888 ATPase, T2SS/T4P/T4SS family virB11
MOO99_RS00315 (465p1_00081) 61804..62388 - 585 WP_241389889 type IV pilus biogenesis protein PilP -
MOO99_RS00320 (465p1_00082) 62375..63490 - 1116 WP_336251292 type 4b pilus protein PilO2 -
MOO99_RS00325 (465p1_00083) 63515..64282 - 768 WP_241391056 DNA adenine methylase -
MOO99_RS00330 (465p1_00084) 64525..65445 + 921 WP_241391020 GNAT family N-acetyltransferase -
MOO99_RS00335 (465p1_00085) 65479..66027 - 549 WP_241391021 tail fiber assembly protein -

Host bacterium

ID   3895 GenBank   NZ_MT039189
Plasmid name   AGR_465|unnamed1 Incompatibility group   -
Plasmid size   181727 bp Coordinate of oriT [Strand]   22640..22722 [+]
Host baterium   Serratia proteamaculans strain AGR_465

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -