Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103450
Name   oriT_AGR_143|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_143
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039181 (21389..21470 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_AGR_143|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGTGCCCCGACACCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2574 GenBank   WP_241389921
Name   Relaxase_MOO85_RS00130_AGR_143|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73479.70 Da        Isoelectric Point: 7.4607

>WP_241389921.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDDDKPDTPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVGLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKEKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
SGMALAENKSGEYLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)


  Protein structure



No available structure.




T4CP

ID   2371 GenBank   WP_241389595
Name   trbC_MOO85_RS00170_AGR_143|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81541.37 Da        Isoelectric Point: 8.3675

>WP_241389595.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDILHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32266..60572

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO85_RS00160 (143p1_00070) 28475..29419 + 945 WP_241389593 hypothetical protein -
MOO85_RS00165 (143p1_00069) 29555..30040 - 486 WP_241389594 phospholipase D-like domain-containing protein -
MOO85_RS00170 (143p1_00068) 30051..32228 - 2178 WP_241389595 F-type conjugative transfer protein TrbC -
MOO85_RS00175 (143p1_00067) 32266..33264 - 999 WP_241389596 hypothetical protein trbB
MOO85_RS00180 (143p1_00066) 33276..34586 - 1311 WP_241389597 conjugal transfer protein TrbA trbA
MOO85_RS00185 (143p1_00065) 34725..35306 - 582 WP_241390358 hypothetical protein -
MOO85_RS00190 (143p1_00064) 35303..37447 - 2145 WP_241389599 DotA/TraY family protein traY
MOO85_RS00195 (143p1_00063) 37512..38084 - 573 WP_241390359 conjugal transfer protein TraX -
MOO85_RS00200 (143p1_00062) 38077..39303 - 1227 WP_241389601 conjugal transfer protein TraW traW
MOO85_RS00205 (143p1_00060) 39444..42518 - 3075 WP_241389602 type IV secretory system conjugative DNA transfer family protein traU
MOO85_RS00210 (143p1_00059) 42521..43126 - 606 WP_241390360 hypothetical protein traT
MOO85_RS00215 (143p1_00058) 43213..43614 - 402 WP_241389604 DUF6750 family protein traR
MOO85_RS00220 (143p1_00057) 43630..44160 - 531 WP_241389605 conjugal transfer protein TraQ traQ
MOO85_RS00225 (143p1_00056) 44169..44918 - 750 WP_241389606 hypothetical protein traP
MOO85_RS00230 (143p1_00055) 44918..46150 - 1233 WP_241389607 conjugal transfer protein TraO traO
MOO85_RS00235 (143p1_00054) 46154..47161 - 1008 WP_241389608 DotH/IcmK family type IV secretion protein traN
MOO85_RS00240 (143p1_00053) 47164..47838 - 675 WP_010895742 DotI/IcmL/TraM family protein traM
MOO85_RS00245 (143p1_00052) 47929..48420 - 492 WP_241389610 hypothetical protein traL
MOO85_RS00250 (143p1_00051) 48430..51666 - 3237 WP_241390361 LPD7 domain-containing protein -
MOO85_RS00255 (143p1_00049) 51809..52075 - 267 WP_241389612 IcmT/TraK family protein traK
MOO85_RS00260 (143p1_00048) 52075..53232 - 1158 WP_241389613 plasmid transfer ATPase TraJ virB11
MOO85_RS00265 (143p1_00047) 53422..54210 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO85_RS00270 (143p1_00046) 54207..54665 - 459 WP_241389614 DotD/TraH family lipoprotein -
MOO85_RS00275 (143p1_00045) 54703..56136 - 1434 WP_241389615 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO85_RS00280 (143p1_00044) 56133..56801 - 669 WP_241390341 prepilin peptidase -
MOO85_RS00285 (143p1_00043) 56801..57286 - 486 WP_241389617 lytic transglycosylase domain-containing protein virB1
MOO85_RS00290 (143p1_00042) 57341..57934 - 594 WP_010895752 type 4 pilus major pilin -
MOO85_RS00295 (143p1_00041) 57970..59022 - 1053 WP_241390342 type II secretion system F family protein -
MOO85_RS00300 (143p1_00040) 59082..60572 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOO85_RS00305 (143p1_00039) 60578..61162 - 585 WP_241390343 type IV pilus biogenesis protein PilP -
MOO85_RS00310 (143p1_00038) 61149..62459 - 1311 WP_241389532 type 4b pilus protein PilO2 -
MOO85_RS00315 (143p1_00037) 62463..63941 - 1479 WP_241390344 PilN family type IVB pilus formation outer membrane protein -
MOO85_RS00320 (143p1_00036) 64123..64548 - 426 WP_241390345 type IV pilus biogenesis protein PilM -
MOO85_RS00325 (143p1_00035) 64548..65561 - 1014 WP_241390346 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3893 GenBank   NZ_MT039181
Plasmid name   AGR_143|unnamed1 Incompatibility group   -
Plasmid size   106005 bp Coordinate of oriT [Strand]   21389..21470 [+]
Host baterium   Serratia proteamaculans strain AGR_143

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -