Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103449
Name   oriT_AGR_1457|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_1457
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039197 (22602..22684 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_1457|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAAGGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2573 GenBank   WP_241390305
Name   Relaxase_MOP13_RS00140_AGR_1457|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73396.69 Da        Isoelectric Point: 7.3282

>WP_241390305.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVDLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKVADDCFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVSECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKCPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRPGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2370 GenBank   WP_241390158
Name   trbC_MOP13_RS00180_AGR_1457|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81527.35 Da        Isoelectric Point: 8.3675

>WP_241390158.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33475..61754

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP13_RS00170 (1457p_00047) 29684..30628 + 945 WP_241389593 hypothetical protein -
MOP13_RS00175 (1457p_00048) 30764..31249 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOP13_RS00180 (1457p_00049) 31260..33437 - 2178 WP_241390158 F-type conjugative transfer protein TrbC -
MOP13_RS00185 (1457p_00050) 33475..34473 - 999 WP_241390308 hypothetical protein trbB
MOP13_RS00190 (1457p_00051) 34486..35796 - 1311 WP_241390430 conjugal transfer protein TrbA trbA
MOP13_RS00195 (1457p_00052) 35925..36503 - 579 WP_241389923 hypothetical protein -
MOP13_RS00200 (1457p_00053) 36500..38794 - 2295 WP_261097221 DotA/TraY family protein -
MOP13_RS00205 (1457p_00054) 38709..39272 - 564 WP_241390311 conjugal transfer protein TraX -
MOP13_RS00210 (1457p_00055) 39265..40491 - 1227 WP_241390312 conjugal transfer protein TraW traW
MOP13_RS00215 (1457p_00057) 40632..43706 - 3075 WP_241390313 type IV secretory system conjugative DNA transfer family protein traU
MOP13_RS00220 (1457p_00058) 43709..44314 - 606 WP_241389875 hypothetical protein traT
MOP13_RS00225 (1457p_00059) 44401..44802 - 402 WP_241389604 DUF6750 family protein traR
MOP13_RS00230 (1457p_00060) 44818..45348 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOP13_RS00235 (1457p_00061) 45357..46100 - 744 WP_241390314 hypothetical protein traP
MOP13_RS00240 (1457p_00062) 46100..47332 - 1233 WP_241389929 conjugal transfer protein TraO traO
MOP13_RS00245 (1457p_00063) 47336..48343 - 1008 WP_241390316 DotH/IcmK family type IV secretion protein traN
MOP13_RS00250 (1457p_00064) 48346..49020 - 675 WP_241390317 DotI/IcmL/TraM family protein traM
MOP13_RS00255 (1457p_00065) 49111..49602 - 492 WP_010895743 hypothetical protein traL
MOP13_RS00260 (1457p_00066) 49612..52848 - 3237 WP_241390318 LPD7 domain-containing protein -
MOP13_RS00265 (1457p_00068) 52991..53257 - 267 WP_241390319 IcmT/TraK family protein traK
MOP13_RS00270 (1457p_00069) 53257..54414 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOP13_RS00275 (1457p_00071) 54604..55392 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP13_RS00280 (1457p_00072) 55389..55847 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP13_RS00285 (1457p_00073) 55885..57318 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP13_RS00290 (1457p_00074) 57315..57983 - 669 WP_241390170 prepilin peptidase -
MOP13_RS00295 (1457p_00075) 57983..58468 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP13_RS00300 (1457p_00076) 58523..59116 - 594 WP_010895752 type 4 pilus major pilin -
MOP13_RS00305 (1457p_00077) 59152..60264 - 1113 WP_241389818 type II secretion system F family protein -
MOP13_RS00310 (1457p_00078) 60264..61754 - 1491 WP_241389888 ATPase, T2SS/T4P/T4SS family virB11
MOP13_RS00315 (1457p_00079) 61760..62344 - 585 WP_241389889 type IV pilus biogenesis protein PilP -
MOP13_RS00320 (1457p_00080) 62331..63641 - 1311 WP_241390279 type 4b pilus protein PilO2 -
MOP13_RS00325 (1457p_00081) 63645..65279 - 1635 WP_241389891 PilN family type IVB pilus formation outer membrane protein -
MOP13_RS00330 (1457p_00082) 65301..65726 - 426 WP_241389892 type IV pilus biogenesis protein PilM -
MOP13_RS00335 (1457p_00083) 65726..66739 - 1014 WP_241390280 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3892 GenBank   NZ_MT039197
Plasmid name   AGR_1457|unnamed1 Incompatibility group   -
Plasmid size   116063 bp Coordinate of oriT [Strand]   22602..22684 [+]
Host baterium   Serratia proteamaculans strain AGR_1457

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -