Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103443
Name   oriT_AGR_142|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_142
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039180 (22258..22340 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_142|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2567 GenBank   WP_241390949
Name   Relaxase_MOP26_RS00135_AGR_142|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73380.61 Da        Isoelectric Point: 7.4747

>WP_241390949.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGLRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2363 GenBank   WP_234591458
Name   trbC_MOP26_RS00150_AGR_142|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81497.36 Da        Isoelectric Point: 8.5425

>WP_234591458.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLKRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   2364 GenBank   WP_010895767
Name   t4cp2_MOP26_RS00350_AGR_142|unnamed1 insolico UniProt ID   _
Length   577 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 577 a.a.        Molecular weight: 64155.03 Da        Isoelectric Point: 5.2094

>WP_010895767.1 MULTISPECIES: ATP-binding protein [Serratia]
MPIFNFRDEDALGKVASVDTTNVIVDVENVEQLKRLQVNHLAVLQSSRPGQHLIGLITQVTRKRGIEDIA
NDGISEQTSELNLCKIALIGTMLDRDGERENVFRRTLESVPEIDANCFSLEGQNLTNFMRTLSSVSADGN
ALTLGKYTLDEHAIAYLNGNKFFQRHAFIGGSTGSGKSWTTAKIIEQMAGLSTANAIVFDLHGEYSPLVG
KGIQHFKVAGPADVEAQRTIDNGVLYLPYWLLSYEALVAMFVDRSDQNAPNQAMIMSREINQAKRKYLED
GDHKEILKHFTVDSPVPFDLDVLMGRLNEINVEMVPGASQGKEKQGDFFGKLARMISRLENKISDRRLGF
IFSGGGDVLNFSWLEKFTTAVLGSSEENGNAGIKIINFSEVPSDVLPLIVSLVARVTFSVQQWTPSELRH
PIALLCDEAHLYMPQRNMAESADDISLDIFERIAKEGRKYGVSLIVISQRPSEVNKTMLSQCSNFVSMRL
TNAEDQGVIKRLLPDSLGGFSDILPTLDTGEALVVGDASLLPSRIRIDEPINKPNSGTVNFWDEWQKPVK
DKRLLIAVENWRKQNIQ

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(144-405)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27933..56223

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP26_RS00140 (142p_00047) 24708..25031 - 324 WP_010895860 hypothetical protein -
MOP26_RS00145 (142p_00046) 25041..25577 - 537 WP_234591459 phospholipase D family protein -
MOP26_RS00150 (142p_00045) 25718..27895 - 2178 WP_234591458 F-type conjugative transfer protein TrbC -
MOP26_RS00155 (142p_00044) 27933..28931 - 999 WP_010895863 hypothetical protein trbB
MOP26_RS00160 (142p_00043) 28944..30254 - 1311 WP_010895864 hypothetical protein trbA
MOP26_RS00165 (142p_00041) 30392..30886 - 495 WP_241390339 hypothetical protein -
MOP26_RS00170 (142p_00040) 30970..33264 - 2295 WP_241389811 DotA/TraY family protein traY
MOP26_RS00175 (142p_00039) 33179..33742 - 564 WP_010895867 conjugal transfer protein TraX -
MOP26_RS00180 (142p_00038) 33735..34961 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOP26_RS00185 (142p_00036) 35102..37480 - 2379 WP_241390950 type IV secretory system conjugative DNA transfer family protein traU
MOP26_RS00190 (142p_00035) 37480..38175 - 696 WP_241390951 hypothetical protein traU
MOP26_RS00195 (142p_00034) 38178..38783 - 606 WP_010895870 TraT traT
MOP26_RS00200 (142p_00033) 38869..39270 - 402 WP_010895871 DUF6750 family protein traR
MOP26_RS00205 (142p_00032) 39286..39816 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOP26_RS00210 (142p_00031) 39825..40568 - 744 WP_010895873 hypothetical protein traP
MOP26_RS00215 (142p_00030) 40568..41800 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOP26_RS00220 (142p_00029) 41805..42812 - 1008 WP_010895741 DotH/IcmK family type IV secretion protein traN
MOP26_RS00225 (142p_00028) 42815..43489 - 675 WP_010895742 DotI/IcmL/TraM family protein traM
MOP26_RS00230 (142p_00027) 43580..44071 - 492 WP_010895743 hypothetical protein traL
MOP26_RS00235 (142p_00026) 44081..47317 - 3237 WP_010895744 LPD7 domain-containing protein -
MOP26_RS00240 (142p_00024) 47460..47726 - 267 WP_010895745 IcmT/TraK family protein traK
MOP26_RS00245 (142p_00023) 47726..48883 - 1158 WP_010895746 plasmid transfer ATPase TraJ virB11
MOP26_RS00250 (142p_00021) 49073..49861 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP26_RS00255 (142p_00020) 49858..50316 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP26_RS00260 (142p_00019) 50354..51787 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP26_RS00265 (142p_00018) 51784..52446 - 663 WP_169558203 prepilin peptidase -
MOP26_RS00270 (142p_00017) 52452..52937 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP26_RS00275 (142p_00016) 52992..53585 - 594 WP_010895752 type 4 pilus major pilin -
MOP26_RS00280 (142p_00015) 53621..54733 - 1113 WP_241389818 type II secretion system F family protein -
MOP26_RS00285 (142p_00014) 54733..56223 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOP26_RS00290 (142p_00013) 56229..56813 - 585 WP_010895755 type IV pilus biogenesis protein PilP -
MOP26_RS00295 (142p_00012) 56800..58110 - 1311 WP_010895756 type 4b pilus protein PilO2 -
MOP26_RS00300 (142p_00011) 58114..59748 - 1635 WP_010895757 PilN family type IVB pilus formation outer membrane protein -
MOP26_RS00305 (142p_00010) 59773..60198 - 426 WP_241390952 type IV pilus biogenesis protein PilM -
MOP26_RS00310 (142p_00009) 60198..61211 - 1014 WP_241389819 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3886 GenBank   NZ_MT039180
Plasmid name   AGR_142|unnamed1 Incompatibility group   -
Plasmid size   150911 bp Coordinate of oriT [Strand]   22258..22340 [+]
Host baterium   Serratia proteamaculans strain AGR_142

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -