Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103438
Name   oriT_pMCR-078001 in_silico
Organism   Enterobacter cloacae complex sp. strain ECL78
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ156802 (278749..279003 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pMCR-078001
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2563 GenBank   WP_272491521
Name   mobH_KR495_RS01575_pMCR-078001 insolico UniProt ID   _
Length   960 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 960 a.a.        Molecular weight: 107734.57 Da        Isoelectric Point: 4.6001

>WP_272491521.1 MobH family relaxase [Enterobacter cloacae complex sp.]
MSMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGGLLSHSLEVAILSLKNAHHSE
LRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWTPSSQSLLDWARENDVVEYEI
HWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYTDGNDFLSKCVRTADFYSTGT
DLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGEVYLNENAFLDFVLNDFELHK
YNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFRNGIGKLEFYNLLKLRWIGLI
FDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDTVETAVLKVNDLGRSSASIDV
DIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESEDESAKDTESHVLVNQLHELL
LSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGSLDGMWIVRDIDDLRLVQLGD
NCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLELPPPRVNAVEEHSGDVEYHG
TDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVGSCATSSSVHNEKLLSEPSEL
PELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAAATSVMASEEPALPPVLEESN
GEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPASPVSGHNLNSKVFASTESDQN
GEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTLYLTQSCVEKFGSQLEAQDLF
NKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(18-214)


  Protein structure



No available structure.




T4CP

ID   2358 GenBank   WP_001284073
Name   traD_KR495_RS01580_pMCR-078001 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 90632..99843

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KR495_RS00510 85742..86458 - 717 WP_000838219 zinc finger-like domain-containing protein -
KR495_RS00515 86455..86655 - 201 WP_000594930 hypothetical protein -
KR495_RS00520 86675..87727 - 1053 WP_001065778 hypothetical protein -
KR495_RS00525 88204..89079 + 876 WP_000594611 RepB family plasmid replication initiator protein -
KR495_RS00530 90228..90581 + 354 WP_000423602 hypothetical protein -
KR495_RS00535 90599..90949 + 351 WP_001447770 type IV conjugative transfer system protein TraL -
KR495_RS01965 90961..91242 + 282 WP_255319156 hypothetical protein traE
KR495_RS01970 91263..91751 + 489 WP_255319157 type IV conjugative transfer system protein TraE traE
KR495_RS00545 91801..93024 + 1224 WP_111962809 type-F conjugative transfer system secretin TraK traK
KR495_RS00550 93026..93487 + 462 WP_000521242 plasmid transfer protein HtdO -
KR495_RS00555 93477..94832 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
KR495_RS00560 94840..95322 + 483 WP_000377632 hypothetical protein -
KR495_RS00565 95441..96193 + 753 WP_182911723 protein-disulfide isomerase HtdT -
KR495_RS00570 96203..97153 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
KR495_RS00575 97162..99843 + 2682 WP_000387412 TraC family protein virb4
KR495_RS00580 100940..101095 + 156 WP_001371891 hypothetical protein -
KR495_RS00585 102392..102553 + 162 WP_001371949 hypothetical protein -

Host bacterium

ID   3881 GenBank   NZ_MZ156802
Plasmid name   pMCR-078001 Incompatibility group   IncHI2
Plasmid size   342942 bp Coordinate of oriT [Strand]   278749..279003 [-]
Host baterium   Enterobacter cloacae complex sp. strain ECL78

Cargo genes

Drug resistance gene   tet(D), blaSHV-12, sul1, blaDHA-1, qnrB4, qacE, ere(A), aac(6')-IIc, blaTEM-1B, dfrA19, aph(3'')-Ib, aph(6)-Id, mcr-9, aph(3')-Ia
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, pbrA, merE, merD, merA, merC, merP, merT, merR, pcoS, pcoE, ncrC, arsC, arsB, arsR, arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -