Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103437
Name   oriT_pSa42-TC5-117k in_silico
Organism   Salmonella enterica subsp. enterica serovar Meleagridis strain Sa42
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MW881231 (38668..38756 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSa42-TC5-117k
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2562 GenBank   WP_001306778
Name   nikB_KHU51_RS00260_pSa42-TC5-117k insolico UniProt ID   Q79VV7
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_001306778.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB Q79VV7


Auxiliary protein

ID   1109 GenBank   WP_001283947
Name   WP_001283947_pSa42-TC5-117k insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2357 GenBank   WP_021497839
Name   trbC_KHU51_RS00265_pSa42-TC5-117k insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86967.19 Da        Isoelectric Point: 7.0662

>WP_021497839.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLKCADPSQDRMIKRSLFSFWPTLFQYEVILESPANGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 44134..108080

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHU51_RS00265 (FPNNMAGG_00051) 41850..44141 - 2292 WP_021497839 F-type conjugative transfer protein TrbC -
KHU51_RS00270 (FPNNMAGG_00052) 44134..45204 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
KHU51_RS00275 (FPNNMAGG_00053) 45223..46431 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
KHU51_RS00280 46723..46875 + 153 WP_001387489 Hok/Gef family protein -
KHU51_RS00285 (FPNNMAGG_00054) 46947..47198 - 252 WP_001291964 hypothetical protein -
KHU51_RS00650 47697..47792 + 96 WP_001303310 DinQ-like type I toxin DqlB -
KHU51_RS00290 47857..48141 - 285 WP_014640317 hypothetical protein -
KHU51_RS00295 (FPNNMAGG_00056) 48365..48574 + 210 WP_000062602 HEAT repeat domain-containing protein -
KHU51_RS00300 (FPNNMAGG_00057) 48646..49308 - 663 WP_000644792 plasmid IncI1-type surface exclusion protein ExcA -
KHU51_RS00305 (FPNNMAGG_00058) 49373..51541 - 2169 WP_077250315 DotA/TraY family protein traY
KHU51_RS00310 (FPNNMAGG_00059) 51638..52222 - 585 WP_001037996 IncI1-type conjugal transfer protein TraX -
KHU51_RS00315 (FPNNMAGG_00060) 52251..53453 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
KHU51_RS00320 (FPNNMAGG_00061) 53420..54034 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
KHU51_RS00325 (FPNNMAGG_00062) 54034..57078 - 3045 WP_001024776 IncI1-type conjugal transfer protein TraU traU
KHU51_RS00330 (FPNNMAGG_00063) 57168..57968 - 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
KHU51_RS00335 (FPNNMAGG_00064) 57952..58140 - 189 WP_001277255 putative conjugal transfer protein TraS -
KHU51_RS00340 (FPNNMAGG_00065) 58204..58608 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
KHU51_RS00345 (FPNNMAGG_00066) 58659..59186 - 528 WP_001055569 conjugal transfer protein TraQ traQ
KHU51_RS00350 (FPNNMAGG_00067) 59186..59890 - 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
KHU51_RS00355 (FPNNMAGG_00068) 59890..61179 - 1290 WP_001271994 conjugal transfer protein TraO traO
KHU51_RS00360 (FPNNMAGG_00069) 61182..62165 - 984 WP_021513963 IncI1-type conjugal transfer protein TraN traN
KHU51_RS00365 (FPNNMAGG_00070) 62176..62868 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
KHU51_RS00370 (FPNNMAGG_00071) 62865..63212 - 348 WP_001055900 conjugal transfer protein traL
KHU51_RS00375 (FPNNMAGG_00072) 63230..66997 - 3768 WP_006855639 LPD7 domain-containing protein -
KHU51_RS00380 (FPNNMAGG_00073) 67087..67638 - 552 WP_000014584 phospholipase D family protein -
KHU51_RS00385 (FPNNMAGG_00074) 67653..67943 - 291 WP_001299214 hypothetical protein traK
KHU51_RS00390 (FPNNMAGG_00075) 67940..68821 - 882 Protein_78 plasmid transfer ATPase TraJ -
KHU51_RS00395 (FPNNMAGG_00076) 68874..69578 - 705 WP_001067858 IS6-like element IS26 family transposase -
KHU51_RS00400 (FPNNMAGG_00077) 69644..69916 - 273 Protein_80 plasmid transfer ATPase TraJ -
KHU51_RS00405 (FPNNMAGG_00078) 69913..70731 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
KHU51_RS00410 (FPNNMAGG_00079) 70728..71186 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
KHU51_RS00415 (FPNNMAGG_00080) 71581..72165 - 585 WP_124770274 histidine phosphatase family protein -
KHU51_RS00420 (FPNNMAGG_00081) 72225..73427 - 1203 WP_000976357 conjugal transfer protein TraF -
KHU51_RS00425 (FPNNMAGG_00082) 73513..74337 - 825 WP_015058912 conjugal transfer protein TraE traE
KHU51_RS00655 74488..75642 - 1155 WP_001139955 site-specific integrase -
KHU51_RS00435 (FPNNMAGG_00083) 75709..77826 - 2118 Protein_87 IncI1-type conjugal transfer protein TraU -
KHU51_RS00440 (FPNNMAGG_00084) 77916..78716 - 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
KHU51_RS00445 (FPNNMAGG_00085) 78700..78888 - 189 WP_001277255 putative conjugal transfer protein TraS -
KHU51_RS00450 (FPNNMAGG_00086) 78952..79356 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
KHU51_RS00455 (FPNNMAGG_00087) 79407..79934 - 528 WP_001055569 conjugal transfer protein TraQ traQ
KHU51_RS00460 (FPNNMAGG_00088) 79934..80638 - 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
KHU51_RS00465 (FPNNMAGG_00089) 80638..81927 - 1290 WP_001271994 conjugal transfer protein TraO traO
KHU51_RS00470 (FPNNMAGG_00090) 81930..82913 - 984 WP_021513963 IncI1-type conjugal transfer protein TraN traN
KHU51_RS00475 (FPNNMAGG_00091) 82924..83616 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
KHU51_RS00480 (FPNNMAGG_00092) 83613..83960 - 348 WP_001055900 conjugal transfer protein traL
KHU51_RS00485 (FPNNMAGG_00093) 83978..87745 - 3768 WP_006855639 LPD7 domain-containing protein -
KHU51_RS00490 (FPNNMAGG_00094) 87835..88386 - 552 WP_000014584 phospholipase D family protein -
KHU51_RS00495 (FPNNMAGG_00095) 88401..88691 - 291 WP_001299214 hypothetical protein traK
KHU51_RS00500 (FPNNMAGG_00096) 88688..89836 - 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
KHU51_RS00505 (FPNNMAGG_00097) 89833..90651 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
KHU51_RS00510 (FPNNMAGG_00098) 90648..91106 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
KHU51_RS00515 (FPNNMAGG_00099) 91501..92085 - 585 WP_124770274 histidine phosphatase family protein -
KHU51_RS00520 92145..92387 - 243 Protein_104 conjugal transfer protein TraF -
KHU51_RS00525 92442..93146 - 705 WP_001067858 IS6-like element IS26 family transposase -
KHU51_RS00530 (FPNNMAGG_00101) 94450..95244 + 795 WP_001096887 aminoglycoside O-phosphotransferase APH(3')-IIIa -
KHU51_RS00535 (FPNNMAGG_00102) 95649..96506 + 858 WP_110085663 TEM family class A beta-lactamase -
KHU51_RS00540 96596..97300 - 705 WP_001067858 IS6-like element IS26 family transposase -
KHU51_RS00545 (FPNNMAGG_00104) 97365..98330 - 966 Protein_109 conjugal transfer protein TraF -
KHU51_RS00550 (FPNNMAGG_00105) 98416..99240 - 825 WP_015058912 conjugal transfer protein TraE traE
KHU51_RS00555 (FPNNMAGG_00106) 99391..100545 - 1155 WP_001139955 site-specific integrase -
KHU51_RS00560 102062..102313 + 252 WP_249335854 hypothetical protein -
KHU51_RS00565 (FPNNMAGG_00108) 102337..103587 - 1251 WP_249335855 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
KHU51_RS00570 (FPNNMAGG_00109) 103587..104243 - 657 WP_001193551 A24 family peptidase -
KHU51_RS00575 (FPNNMAGG_00110) 104228..104788 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
KHU51_RS00580 (FPNNMAGG_00111) 104798..105412 - 615 WP_000908228 type 4 pilus major pilin -
KHU51_RS00585 (FPNNMAGG_00112) 105429..106514 - 1086 WP_001208803 type II secretion system F family protein -
KHU51_RS00590 (FPNNMAGG_00113) 106527..108080 - 1554 WP_000362204 ATPase, T2SS/T4P/T4SS family virB11
KHU51_RS00595 (FPNNMAGG_00114) 108091..108543 - 453 WP_001247333 type IV pilus biogenesis protein PilP -
KHU51_RS00600 (FPNNMAGG_00115) 108530..109825 - 1296 WP_000752776 type 4b pilus protein PilO2 -
KHU51_RS00605 (FPNNMAGG_00116) 109818..111500 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
KHU51_RS00610 (FPNNMAGG_00117) 111514..111951 - 438 WP_000539811 type IV pilus biogenesis protein PilM -
KHU51_RS00615 (FPNNMAGG_00118) 111951..113018 - 1068 WP_021513968 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3880 GenBank   NZ_MW881231
Plasmid name   pSa42-TC5-117k Incompatibility group   IncI1
Plasmid size   117221 bp Coordinate of oriT [Strand]   38668..38756 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Meleagridis strain Sa42

Cargo genes

Drug resistance gene   blaCTX-M-24, aph(3')-III, blaTEM-116
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -