Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103428
Name   oriT_pIB2020_ColE_MCR in_silico
Organism   Enterobacter kobei strain IB2020
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP059482 (139..183 [-], 45 nt)
oriT length   45 nt
IRs (inverted repeats)      7..13, 25..31  (CGTTTCT..AGAAACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 45 nt

>oriT_pIB2020_ColE_MCR
GCGCACCGTTTCTGAACGAAGTGAAGAAACGTCTAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2555 GenBank   WP_040113349
Name   mobA_H1R18_RS25065_pIB2020_ColE_MCR insolico UniProt ID   A0A734ICP3
Length   365 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 365 a.a.        Molecular weight: 42217.70 Da        Isoelectric Point: 6.3344

>WP_040113349.1 MULTISPECIES: mobilization protein MobA [Enterobacterales]
MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNMPAWAAHKPTEFWKAADTFERANGCT
YREIEIALPRELKPEQRLELVRDFVRQEIGDRHAYQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDP
QQYFKRANSKAPERGGAKKARFGETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIERE
PERHLGAGQVQRFDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQ
EDTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQRQQEKEQQRLA
EQTRQKPDKGWSFSR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(42-218)


  Protein structure


Source ID Structure
AlphaFold DB A0A734ICP3


Host bacterium

ID   3871 GenBank   NZ_CP059482
Plasmid name   pIB2020_ColE_MCR Incompatibility group   ColE10
Plasmid size   12808 bp Coordinate of oriT [Strand]   139..183 [-]
Host baterium   Enterobacter kobei strain IB2020

Cargo genes

Drug resistance gene   mcr-4.3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -