Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103419 |
| Name | oriT_pKpQIL  |
| Organism | Klebsiella pneumoniae strain 42B isolate conti53RC_42B |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT809701 (91220..91269 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKpQIL
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2548 | GenBank | WP_210205478 |
| Name | traI_KBQ03_RS00325_pKpQIL |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190821.02 Da Isoelectric Point: 6.4089
>WP_210205478.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2336 | GenBank | WP_004152630 |
| Name | traD_KBQ03_RS00330_pKpQIL |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85951.02 Da Isoelectric Point: 5.0632
>WP_004152630.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 62873..91827
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KBQ03_RS00330 | 62873..65182 | - | 2310 | WP_004152630 | type IV conjugative transfer system coupling protein TraD | virb4 |
| KBQ03_RS00335 | 65311..66000 | - | 690 | WP_004198206 | hypothetical protein | - |
| KBQ03_RS00340 | 66192..66923 | - | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| KBQ03_RS00345 | 67107..67655 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| KBQ03_RS00350 | 67668..70517 | - | 2850 | WP_093997975 | conjugal transfer mating-pair stabilization protein TraG | traG |
| KBQ03_RS00355 | 70517..71896 | - | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| KBQ03_RS00360 | 71874..72317 | - | 444 | WP_004152679 | F-type conjugal transfer protein TrbF | - |
| KBQ03_RS00365 | 72363..72920 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| KBQ03_RS00370 | 72892..73131 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| KBQ03_RS00375 | 73142..73894 | - | 753 | WP_004152686 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| KBQ03_RS00380 | 73915..74241 | - | 327 | WP_004152685 | hypothetical protein | - |
| KBQ03_RS00700 | 74287..74472 | - | 186 | WP_004152684 | hypothetical protein | - |
| KBQ03_RS00385 | 74469..74705 | - | 237 | WP_004152683 | conjugal transfer protein TrbE | - |
| KBQ03_RS00390 | 74737..76692 | - | 1956 | WP_015060081 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| KBQ03_RS00395 | 76740..77378 | - | 639 | WP_004152682 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| KBQ03_RS00400 | 77391..78380 | - | 990 | WP_011977785 | conjugal transfer pilus assembly protein TraU | traU |
| KBQ03_RS00405 | 78377..78766 | - | 390 | WP_004152508 | hypothetical protein | - |
| KBQ03_RS00410 | 78808..79434 | - | 627 | WP_009309871 | type-F conjugative transfer system protein TraW | traW |
| KBQ03_RS00415 | 79434..79823 | - | 390 | WP_004152506 | type-F conjugative transfer system protein TrbI | - |
| KBQ03_RS00420 | 79823..82462 | - | 2640 | WP_004152505 | type IV secretion system protein TraC | virb4 |
| KBQ03_RS00425 | 82534..82932 | - | 399 | WP_011977783 | hypothetical protein | - |
| KBQ03_RS00430 | 82940..83329 | - | 390 | WP_004153076 | hypothetical protein | - |
| KBQ03_RS00435 | 83372..83776 | - | 405 | WP_004152503 | hypothetical protein | - |
| KBQ03_RS00440 | 83843..84154 | - | 312 | WP_004152502 | hypothetical protein | - |
| KBQ03_RS00445 | 84155..84373 | - | 219 | WP_004152501 | hypothetical protein | - |
| KBQ03_RS00450 | 84479..84889 | - | 411 | WP_004152499 | hypothetical protein | - |
| KBQ03_RS00455 | 85021..85605 | - | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| KBQ03_RS00460 | 85719..87143 | - | 1425 | WP_040179666 | F-type conjugal transfer pilus assembly protein TraB | traB |
| KBQ03_RS00465 | 87143..87883 | - | 741 | WP_004152601 | type-F conjugative transfer system secretin TraK | traK |
| KBQ03_RS00470 | 87870..88436 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| KBQ03_RS00475 | 88456..88761 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| KBQ03_RS00480 | 88775..89143 | - | 369 | WP_004152496 | type IV conjugative transfer system pilin TraA | - |
| KBQ03_RS00485 | 89197..89583 | - | 387 | WP_004152495 | TraY domain-containing protein | - |
| KBQ03_RS00490 | 89662..90348 | - | 687 | WP_004152494 | transcriptional regulator TraJ family protein | - |
| KBQ03_RS00495 | 90522..90920 | - | 399 | WP_004152493 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| KBQ03_RS00500 | 91342..91827 | + | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| KBQ03_RS00505 | 91860..92189 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| KBQ03_RS00510 | 92222..93042 | - | 821 | Protein_103 | DUF932 domain-containing protein | - |
| KBQ03_RS00515 | 93862..94695 | - | 834 | WP_004152751 | N-6 DNA methylase | - |
| KBQ03_RS00520 | 94746..94892 | - | 147 | WP_004152750 | hypothetical protein | - |
| KBQ03_RS00525 | 94987..95334 | - | 348 | WP_004152749 | hypothetical protein | - |
| KBQ03_RS00530 | 95391..95744 | - | 354 | WP_004152748 | hypothetical protein | - |
| KBQ03_RS00535 | 96374..96724 | - | 351 | WP_004153414 | hypothetical protein | - |
Host bacterium
| ID | 3862 | GenBank | NZ_MT809701 |
| Plasmid name | pKpQIL | Incompatibility group | IncFIB |
| Plasmid size | 115662 bp | Coordinate of oriT [Strand] | 91220..91269 [+] |
| Host baterium | Klebsiella pneumoniae strain 42B isolate conti53RC_42B |
Cargo genes
| Drug resistance gene | blaTEM-1A, blaOXA-9, blaKPC-31 |
| Virulence gene | - |
| Metal resistance gene | merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |