Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103417 |
| Name | oriT_pKpQIL  |
| Organism | Klebsiella pneumoniae strain 2B |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT809695 (89207..89256 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKpQIL
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2547 | GenBank | WP_210205417 |
| Name | traI_KBP93_RS00315_pKpQIL |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190467.48 Da Isoelectric Point: 6.3379
>WP_210205417.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2335 | GenBank | WP_032495268 |
| Name | traD_KBP93_RS00320_pKpQIL |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85986.98 Da Isoelectric Point: 5.0632
>WP_032495268.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60817..89814
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KBP93_RS00320 | 60817..63129 | - | 2313 | WP_032495268 | type IV conjugative transfer system coupling protein TraD | virb4 |
| KBP93_RS00325 | 63258..63947 | - | 690 | WP_004198206 | hypothetical protein | - |
| KBP93_RS00330 | 64139..64870 | - | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| KBP93_RS00335 | 65054..65602 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| KBP93_RS00340 | 65615..68464 | - | 2850 | WP_004152624 | conjugal transfer mating-pair stabilization protein TraG | traG |
| KBP93_RS00345 | 68464..69843 | - | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| KBP93_RS00350 | 69821..70264 | - | 444 | WP_004152679 | F-type conjugal transfer protein TrbF | - |
| KBP93_RS00355 | 70310..70867 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| KBP93_RS00360 | 70839..71078 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| KBP93_RS00365 | 71089..71841 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| KBP93_RS00370 | 71862..72188 | - | 327 | WP_004152676 | hypothetical protein | - |
| KBP93_RS00375 | 72201..72449 | - | 249 | WP_004152675 | hypothetical protein | - |
| KBP93_RS00380 | 72427..72681 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| KBP93_RS00385 | 72713..74668 | - | 1956 | WP_004152673 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| KBP93_RS00390 | 74727..75365 | - | 639 | WP_004152682 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| KBP93_RS00395 | 75378..76367 | - | 990 | WP_011977785 | conjugal transfer pilus assembly protein TraU | traU |
| KBP93_RS00400 | 76364..76753 | - | 390 | WP_004152508 | hypothetical protein | - |
| KBP93_RS00405 | 76795..77421 | - | 627 | WP_004152507 | type-F conjugative transfer system protein TraW | traW |
| KBP93_RS00410 | 77421..77810 | - | 390 | WP_004152506 | type-F conjugative transfer system protein TrbI | - |
| KBP93_RS00415 | 77810..80449 | - | 2640 | WP_004152505 | type IV secretion system protein TraC | virb4 |
| KBP93_RS00420 | 80521..80919 | - | 399 | WP_011977783 | hypothetical protein | - |
| KBP93_RS00425 | 80927..81316 | - | 390 | WP_004153076 | hypothetical protein | - |
| KBP93_RS00430 | 81359..81763 | - | 405 | WP_004152503 | hypothetical protein | - |
| KBP93_RS00435 | 81830..82141 | - | 312 | WP_004152502 | hypothetical protein | - |
| KBP93_RS00440 | 82142..82360 | - | 219 | WP_004152501 | hypothetical protein | - |
| KBP93_RS00445 | 82466..82876 | - | 411 | WP_004152499 | hypothetical protein | - |
| KBP93_RS00450 | 83008..83592 | - | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| KBP93_RS00455 | 83706..85130 | - | 1425 | WP_004155033 | F-type conjugal transfer pilus assembly protein TraB | traB |
| KBP93_RS00460 | 85130..85870 | - | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| KBP93_RS00465 | 85857..86423 | - | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| KBP93_RS00470 | 86443..86748 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| KBP93_RS00475 | 86762..87130 | - | 369 | WP_004152496 | type IV conjugative transfer system pilin TraA | - |
| KBP93_RS00480 | 87184..87570 | - | 387 | WP_004152495 | TraY domain-containing protein | - |
| KBP93_RS00485 | 87649..88335 | - | 687 | WP_004152494 | transcriptional regulator TraJ family protein | - |
| KBP93_RS00490 | 88509..88907 | - | 399 | WP_004152493 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| KBP93_RS00495 | 89329..89814 | + | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| KBP93_RS00500 | 89847..90176 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| KBP93_RS00505 | 90209..91030 | - | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| KBP93_RS00510 | 91850..92683 | - | 834 | WP_004152751 | N-6 DNA methylase | - |
| KBP93_RS00515 | 92734..92880 | - | 147 | WP_004152750 | hypothetical protein | - |
| KBP93_RS00520 | 92975..93322 | - | 348 | WP_004152749 | hypothetical protein | - |
| KBP93_RS00525 | 93379..93732 | - | 354 | WP_004152748 | hypothetical protein | - |
| KBP93_RS00530 | 94362..94712 | - | 351 | WP_004153414 | hypothetical protein | - |
Host bacterium
| ID | 3860 | GenBank | NZ_MT809695 |
| Plasmid name | pKpQIL | Incompatibility group | IncFIB |
| Plasmid size | 113648 bp | Coordinate of oriT [Strand] | 89207..89256 [+] |
| Host baterium | Klebsiella pneumoniae strain 2B |
Cargo genes
| Drug resistance gene | blaTEM-1A, blaOXA-9, blaKPC-29 |
| Virulence gene | - |
| Metal resistance gene | merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |