Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103411 |
| Name | oriT_pKpQIL  |
| Organism | Klebsiella pneumoniae strain 40B isolate |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT809700 (89146..89195 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKpQIL
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2543 | GenBank | WP_093944916 |
| Name | traI_KBP99_RS00315_pKpQIL |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190478.48 Da Isoelectric Point: 6.3660
>WP_093944916.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGMFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGASGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGMFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGASGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQKSVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2328 | GenBank | WP_032495268 |
| Name | traD_KBP99_RS00320_pKpQIL |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85986.98 Da Isoelectric Point: 5.0632
>WP_032495268.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60828..89753
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KBP99_RS00320 | 60828..63140 | - | 2313 | WP_032495268 | type IV conjugative transfer system coupling protein TraD | virb4 |
| KBP99_RS00325 | 63269..63958 | - | 690 | WP_004198206 | hypothetical protein | - |
| KBP99_RS00330 | 64150..64881 | - | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| KBP99_RS00335 | 65065..65613 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| KBP99_RS00340 | 65626..68475 | - | 2850 | WP_004152624 | conjugal transfer mating-pair stabilization protein TraG | traG |
| KBP99_RS00345 | 68475..69854 | - | 1380 | WP_004165169 | conjugal transfer pilus assembly protein TraH | traH |
| KBP99_RS00350 | 69832..70275 | - | 444 | WP_004152679 | F-type conjugal transfer protein TrbF | - |
| KBP99_RS00355 | 70321..70878 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| KBP99_RS00360 | 70850..71089 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| KBP99_RS00365 | 71100..71852 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| KBP99_RS00370 | 71873..72199 | - | 327 | WP_004152676 | hypothetical protein | - |
| KBP99_RS00375 | 72212..72460 | - | 249 | WP_004152675 | hypothetical protein | - |
| KBP99_RS00380 | 72438..72692 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| KBP99_RS00385 | 72724..74679 | - | 1956 | WP_004152673 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| KBP99_RS00390 | 74738..75376 | - | 639 | WP_004152672 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| KBP99_RS00395 | 75389..76378 | - | 990 | WP_011977785 | conjugal transfer pilus assembly protein TraU | traU |
| KBP99_RS00400 | 76375..76764 | - | 390 | WP_004152508 | hypothetical protein | - |
| KBP99_RS00405 | 76806..77432 | - | 627 | WP_004152507 | type-F conjugative transfer system protein TraW | traW |
| KBP99_RS00410 | 77432..77821 | - | 390 | WP_004152506 | type-F conjugative transfer system protein TrbI | - |
| KBP99_RS00415 | 77821..80460 | - | 2640 | WP_004152505 | type IV secretion system protein TraC | virb4 |
| KBP99_RS00420 | 80532..80930 | - | 399 | WP_011977783 | hypothetical protein | - |
| KBP99_RS00425 | 80938..81327 | - | 390 | WP_004153076 | hypothetical protein | - |
| KBP99_RS00430 | 81370..81774 | - | 405 | WP_004152503 | hypothetical protein | - |
| KBP99_RS00435 | 81841..82152 | - | 312 | WP_004152502 | hypothetical protein | - |
| KBP99_RS00440 | 82153..82371 | - | 219 | WP_004152501 | hypothetical protein | - |
| KBP99_RS00445 | 82477..82887 | - | 411 | WP_004152499 | hypothetical protein | - |
| KBP99_RS00450 | 83019..83603 | - | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| KBP99_RS00455 | 83717..85141 | - | 1425 | WP_004155033 | F-type conjugal transfer pilus assembly protein TraB | traB |
| KBP99_RS00460 | 85141..85881 | - | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| KBP99_RS00465 | 85868..86434 | - | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| KBP99_RS00470 | 86454..86759 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| KBP99_RS00475 | 86773..87141 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| KBP99_RS00480 | 87203..87409 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| KBP99_RS00485 | 87542..88261 | - | 720 | WP_023328286 | hypothetical protein | - |
| KBP99_RS00490 | 88461..88877 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| KBP99_RS00495 | 89268..89753 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| KBP99_RS00500 | 89786..90115 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| KBP99_RS00505 | 90148..90969 | - | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| KBP99_RS00510 | 91789..92622 | - | 834 | WP_004152751 | N-6 DNA methylase | - |
| KBP99_RS00515 | 92673..92819 | - | 147 | WP_004152750 | hypothetical protein | - |
| KBP99_RS00520 | 92914..93261 | - | 348 | WP_004152749 | hypothetical protein | - |
| KBP99_RS00525 | 93318..93671 | - | 354 | WP_004152748 | hypothetical protein | - |
| KBP99_RS00530 | 94301..94651 | - | 351 | WP_004153414 | hypothetical protein | - |
Host bacterium
| ID | 3854 | GenBank | NZ_MT809700 |
| Plasmid name | pKpQIL | Incompatibility group | IncFIB |
| Plasmid size | 113531 bp | Coordinate of oriT [Strand] | 89146..89195 [+] |
| Host baterium | Klebsiella pneumoniae strain 40B isolate |
Cargo genes
| Drug resistance gene | blaTEM-1A, blaOXA-9, blaKPC-40 |
| Virulence gene | - |
| Metal resistance gene | merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |