Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103408 |
| Name | oriT_pKpQIL  |
| Organism | Klebsiella pneumoniae strain 1B |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT809694 (88688..88737 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKpQIL
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2540 | GenBank | WP_210204492 |
| Name | traI_KBP88_RS00315_pKpQIL |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190528.48 Da Isoelectric Point: 6.2072
>WP_210204492.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTSFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2325 | GenBank | WP_077273343 |
| Name | traD_KBP88_RS00320_pKpQIL |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85877.82 Da Isoelectric Point: 4.9652
>WP_077273343.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60808..89295
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KBP88_RS00320 | 60808..63117 | - | 2310 | WP_077273343 | type IV conjugative transfer system coupling protein TraD | virb4 |
| KBP88_RS00325 | 63246..63935 | - | 690 | WP_004198206 | hypothetical protein | - |
| KBP88_RS00330 | 64127..64858 | - | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| KBP88_RS00335 | 65042..65590 | - | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| KBP88_RS00340 | 65603..68452 | - | 2850 | WP_093984080 | conjugal transfer mating-pair stabilization protein TraG | traG |
| KBP88_RS00345 | 68452..69822 | - | 1371 | WP_004178055 | conjugal transfer pilus assembly protein TraH | traH |
| KBP88_RS00350 | 69809..70237 | - | 429 | WP_004152689 | hypothetical protein | - |
| KBP88_RS00355 | 70230..70802 | - | 573 | WP_004152688 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| KBP88_RS00360 | 70774..71013 | - | 240 | WP_004152687 | type-F conjugative transfer system pilin chaperone TraQ | - |
| KBP88_RS00365 | 71024..71776 | - | 753 | WP_004152686 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| KBP88_RS00370 | 71797..72123 | - | 327 | WP_004152685 | hypothetical protein | - |
| KBP88_RS00665 | 72169..72354 | - | 186 | WP_004152684 | hypothetical protein | - |
| KBP88_RS00375 | 72351..72587 | - | 237 | WP_004152683 | conjugal transfer protein TrbE | - |
| KBP88_RS00380 | 72619..74574 | - | 1956 | WP_156904056 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| KBP88_RS00385 | 74622..75260 | - | 639 | WP_004152682 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| KBP88_RS00390 | 75273..76232 | - | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| KBP88_RS00395 | 76276..76902 | - | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| KBP88_RS00400 | 76902..77291 | - | 390 | WP_004152591 | type-F conjugative transfer system protein TrbI | - |
| KBP88_RS00405 | 77291..79930 | - | 2640 | WP_032431388 | type IV secretion system protein TraC | virb4 |
| KBP88_RS00410 | 80002..80400 | - | 399 | WP_011977783 | hypothetical protein | - |
| KBP88_RS00415 | 80408..80797 | - | 390 | WP_004153076 | hypothetical protein | - |
| KBP88_RS00420 | 80840..81244 | - | 405 | WP_004152503 | hypothetical protein | - |
| KBP88_RS00425 | 81311..81622 | - | 312 | WP_004152502 | hypothetical protein | - |
| KBP88_RS00430 | 81623..81841 | - | 219 | WP_004152501 | hypothetical protein | - |
| KBP88_RS00435 | 81947..82357 | - | 411 | WP_004152499 | hypothetical protein | - |
| KBP88_RS00440 | 82489..83073 | - | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| KBP88_RS00445 | 83187..84611 | - | 1425 | WP_040179666 | F-type conjugal transfer pilus assembly protein TraB | traB |
| KBP88_RS00450 | 84611..85351 | - | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| KBP88_RS00455 | 85338..85904 | - | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| KBP88_RS00460 | 85924..86229 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| KBP88_RS00465 | 86243..86611 | - | 369 | WP_004152496 | type IV conjugative transfer system pilin TraA | - |
| KBP88_RS00470 | 86665..87051 | - | 387 | WP_004152495 | TraY domain-containing protein | - |
| KBP88_RS00475 | 87130..87816 | - | 687 | WP_004152494 | transcriptional regulator TraJ family protein | - |
| KBP88_RS00480 | 87990..88388 | - | 399 | WP_004152493 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| KBP88_RS00485 | 88810..89295 | + | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| KBP88_RS00490 | 89328..89657 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| KBP88_RS00495 | 89690..90511 | - | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| KBP88_RS00500 | 91331..92164 | - | 834 | WP_004152751 | N-6 DNA methylase | - |
| KBP88_RS00505 | 92215..92361 | - | 147 | WP_004152750 | hypothetical protein | - |
| KBP88_RS00510 | 92456..92803 | - | 348 | WP_004152749 | hypothetical protein | - |
| KBP88_RS00515 | 92860..93213 | - | 354 | WP_004152748 | hypothetical protein | - |
| KBP88_RS00520 | 93843..94193 | - | 351 | WP_004153414 | hypothetical protein | - |
Host bacterium
| ID | 3851 | GenBank | NZ_MT809694 |
| Plasmid name | pKpQIL | Incompatibility group | IncFIB |
| Plasmid size | 113130 bp | Coordinate of oriT [Strand] | 88688..88737 [+] |
| Host baterium | Klebsiella pneumoniae strain 1B |
Cargo genes
| Drug resistance gene | blaTEM-1A, blaOXA-9, blaKPC-3 |
| Virulence gene | - |
| Metal resistance gene | merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |