Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103401 |
| Name | oriT_pSZ10R-tetX4  |
| Organism | Escherichia sp. strain SZ10R |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MW940628 (2912..3005 [+], 94 nt) |
| oriT length | 94 nt |
| IRs (inverted repeats) | 72..77, 84..89 (AAAAAA..TTTTTT) 72..77, 83..88 (AAAAAA..TTTTTT) 26..33, 36..43 (AGCGTGAT..ATCACGCT) 12..18, 30..36 (TAAATCA..TGATTTA) |
| Location of nic site | 54..55 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 94 nt
>oriT_pSZ10R-tetX4
TTTTTTTCTTTTAAATCAGTGAGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTAACGGGATAAAAAATCATCTTTTTTTGGTAG
TTTTTTTCTTTTAAATCAGTGAGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTAACGGGATAAAAAATCATCTTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2538 | GenBank | WP_223820406 |
| Name | Mob_Pre_KSF49_RS00180_pSZ10R-tetX4 |
UniProt ID | _ |
| Length | 103 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 103 a.a. Molecular weight: 11864.27 Da Isoelectric Point: 7.7517
>WP_223820406.1 MULTISPECIES: plasmid recombination protein [Gammaproteobacteria]
MGTVAAALQHCYRDRETPNADQERTPDNDHLAARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWW
QTASADQQREFFKRSTEWLAACRKFRCSATAMN
MGTVAAALQHCYRDRETPNADQERTPDNDHLAARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWW
QTASADQQREFFKRSTEWLAACRKFRCSATAMN
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 3844 | GenBank | NZ_MW940628 |
| Plasmid name | pSZ10R-tetX4 | Incompatibility group | IncFIB |
| Plasmid size | 130185 bp | Coordinate of oriT [Strand] | 2912..3005 [+] |
| Host baterium | Escherichia sp. strain SZ10R |
Cargo genes
| Drug resistance gene | erm(42), tet(X4), floR, mph(A), blaTEM-1B, tet(M), tet(A), dfrA12, aadA2, cmlA1, ant(3'')-Ia, sul3, aph(3'')-Ib, aph(6)-Id |
| Virulence gene | - |
| Metal resistance gene | silP, silA, silB, silF, silC, silR, silS, silE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |