Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103393 |
| Name | oriT_pKpQIL  |
| Organism | Klebsiella pneumoniae strain 26B isolate NODE_32_26B |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT809699 (10675..10724 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKpQIL
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2533 | GenBank | WP_210205203 |
| Name | traI_KBP92_RS00260_pKpQIL |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190483.37 Da Isoelectric Point: 6.1397
>WP_210205203.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVPMADIDTTI
QTQIRSGQLLNAPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTQAQEKAIQ
KALSEGETLPAGQPASLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2310 | GenBank | WP_004152630 |
| Name | traD_KBP92_RS00255_pKpQIL |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85951.02 Da Isoelectric Point: 5.0632
>WP_004152630.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 10117..39111
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KBP92_RS00045 | 5219..5569 | + | 351 | WP_004153414 | hypothetical protein | - |
| KBP92_RS00050 | 6199..6552 | + | 354 | WP_004152748 | hypothetical protein | - |
| KBP92_RS00055 | 6609..6956 | + | 348 | WP_004152749 | hypothetical protein | - |
| KBP92_RS00060 | 7051..7197 | + | 147 | WP_004152750 | hypothetical protein | - |
| KBP92_RS00065 | 7248..8081 | + | 834 | WP_004152751 | N-6 DNA methylase | - |
| KBP92_RS00070 | 8901..9722 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| KBP92_RS00075 | 9755..10084 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| KBP92_RS00080 | 10117..10602 | - | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| KBP92_RS00085 | 11024..11422 | + | 399 | WP_004152493 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| KBP92_RS00090 | 11596..12282 | + | 687 | WP_004152494 | transcriptional regulator TraJ family protein | - |
| KBP92_RS00095 | 12361..12747 | + | 387 | WP_004152495 | TraY domain-containing protein | - |
| KBP92_RS00100 | 12801..13169 | + | 369 | WP_004152496 | type IV conjugative transfer system pilin TraA | - |
| KBP92_RS00105 | 13183..13488 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| KBP92_RS00110 | 13508..14074 | + | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| KBP92_RS00115 | 14061..14801 | + | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| KBP92_RS00120 | 14801..16225 | + | 1425 | WP_004155033 | F-type conjugal transfer pilus assembly protein TraB | traB |
| KBP92_RS00125 | 16339..16923 | + | 585 | WP_004161368 | type IV conjugative transfer system lipoprotein TraV | traV |
| KBP92_RS00130 | 17055..17465 | + | 411 | WP_004152499 | hypothetical protein | - |
| KBP92_RS00135 | 17571..17789 | + | 219 | WP_004152501 | hypothetical protein | - |
| KBP92_RS00140 | 17790..18101 | + | 312 | WP_004152502 | hypothetical protein | - |
| KBP92_RS00145 | 18168..18572 | + | 405 | WP_004152503 | hypothetical protein | - |
| KBP92_RS00150 | 18615..19004 | + | 390 | WP_004153076 | hypothetical protein | - |
| KBP92_RS00155 | 19012..19410 | + | 399 | WP_011977783 | hypothetical protein | - |
| KBP92_RS00160 | 19482..22121 | + | 2640 | WP_004152505 | type IV secretion system protein TraC | virb4 |
| KBP92_RS00165 | 22121..22510 | + | 390 | WP_004152506 | type-F conjugative transfer system protein TrbI | - |
| KBP92_RS00170 | 22510..23136 | + | 627 | WP_004152507 | type-F conjugative transfer system protein TraW | traW |
| KBP92_RS00175 | 23178..23567 | + | 390 | WP_004152508 | hypothetical protein | - |
| KBP92_RS00180 | 23564..24553 | + | 990 | WP_011977785 | conjugal transfer pilus assembly protein TraU | traU |
| KBP92_RS00185 | 24566..25204 | + | 639 | WP_004152672 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| KBP92_RS00190 | 25263..27218 | + | 1956 | WP_004152673 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| KBP92_RS00195 | 27250..27504 | + | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| KBP92_RS00200 | 27482..27730 | + | 249 | WP_004152675 | hypothetical protein | - |
| KBP92_RS00205 | 27743..28069 | + | 327 | WP_004152676 | hypothetical protein | - |
| KBP92_RS00210 | 28090..28842 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| KBP92_RS00215 | 28853..29092 | + | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| KBP92_RS00220 | 29064..29621 | + | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| KBP92_RS00225 | 29667..30110 | + | 444 | WP_004152679 | F-type conjugal transfer protein TrbF | - |
| KBP92_RS00230 | 30088..31467 | + | 1380 | WP_004165169 | conjugal transfer pilus assembly protein TraH | traH |
| KBP92_RS00235 | 31467..34316 | + | 2850 | WP_004152624 | conjugal transfer mating-pair stabilization protein TraG | traG |
| KBP92_RS00240 | 34329..34877 | + | 549 | WP_004152623 | conjugal transfer entry exclusion protein TraS | - |
| KBP92_RS00245 | 35061..35792 | + | 732 | WP_004152622 | conjugal transfer complement resistance protein TraT | - |
| KBP92_RS00250 | 35984..36673 | + | 690 | WP_004198206 | hypothetical protein | - |
| KBP92_RS00255 | 36802..39111 | + | 2310 | WP_004152630 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 3836 | GenBank | NZ_MT809699 |
| Plasmid name | pKpQIL | Incompatibility group | IncFII |
| Plasmid size | 109606 bp | Coordinate of oriT [Strand] | 10675..10724 [-] |
| Host baterium | Klebsiella pneumoniae strain 26B isolate NODE_32_26B |
Cargo genes
| Drug resistance gene | blaKPC-3, blaOXA-9, blaTEM-1A |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |