Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103389
• Name: oriT1_pUR2940
• Organism: Staphylococcus aureus strain ST398 isolate C2940
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MW367469 ( 13822..13862 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT1_pUR2940
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_Pre_JZ796_RS00070_pUR2940

• ID: 2530
• GenBank: WP_032494831
• Name: Mob_Pre_JZ796_RS00070_pUR2940
• UniProt ID: _
• Length: 403 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 403 a.a.
• Molecular weight: 46964.92 Da
• Isoelectric Point: 9.2169

>WP_032494831.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSFVVARMQKMKSGNLVGVGNHNQRNTDNHSNKDIDVERSYLNYDLVNRTENYKRDIEQFINDNKSSSRA
VRKDAVLINEWIITSDNQFFKDKDDKEIKDFFEQSKDYFAEKFGDENIRYAQVHLDETTPHMHLGIVPFN
AEKKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEMTTTLEQRKS
EVLALSNDKTATIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIISKDKLNALIQGYE
DLYSSNERLKKYAETDLPKEITKVKGKYSELVKEYNDLVRKSNRNIETLEELEKENKSLKNEIKDIYKGF
KAYFKDSKQVTTEQVKTLVGGVVDKVKEFVKGGEFEKIHRNETRTRDRDELSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 3832
• GenBank: NZ_MW367469
• Plasmid name: pUR2940
• Incompatibility group: -
• Plasmid size: 23700 bp
• Coordinate of oriT [Strand]: 12550..12590 [+]; 13822..13862 [+]
• Host baterium: Staphylococcus aureus strain ST398 isolate C2940

Cargo genes

• Drug resistance gene: erm(C), tet(L), dfrK, erm(T)
• Virulence gene: -
• Metal resistance gene: mco
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21