Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103376 |
| Name | oriT_pEh-GE2  |
| Organism | Enterococcus hirae strain GE2 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT683616 (21233..21436 [-], 204 nt) |
| oriT length | 204 nt |
| IRs (inverted repeats) | 125..130, 143..148 (AAATCC..GGATTT) 64..71, 83..90 (TACCCCCC..GGGGGGTA) 67..72, 83..88 (CCCCCC..GGGGGG) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TTTGGTTACA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 204 nt
>oriT_pEh-GE2
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTACCCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGAT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTACCCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2525 | GenBank | WP_000398284 |
| Name | Rep_trans_J1790_RS00110_pEh-GE2 |
UniProt ID | A0A3P3PY43 |
| Length | 401 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47398.10 Da Isoelectric Point: 6.5091
>WP_000398284.1 MULTISPECIES: MobT family relaxase [Bacteria]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3P3PY43 |
T4CP
| ID | 2299 | GenBank | WP_000813488 |
| Name | tcpA_J1790_RS00120_pEh-GE2 |
UniProt ID | _ |
| Length | 461 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>WP_000813488.1 MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Bacteria]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 13060..23541
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J1790_RS00050 | 8261..8941 | + | 681 | WP_001015311 | IS6-like element IS1216 family transposase | - |
| J1790_RS00055 | 9069..10028 | + | 960 | WP_000222577 | IS30-like element IS1252 family transposase | - |
| J1790_RS00060 | 10461..11033 | - | 573 | WP_000170424 | recombinase family protein | - |
| J1790_RS00065 | 11049..11654 | - | 606 | WP_000599739 | protein adenylyltransferase Fic | - |
| J1790_RS00070 | 11852..12532 | - | 681 | WP_001015311 | IS6-like element IS1216 family transposase | - |
| J1790_RS00075 | 12608..13063 | - | 456 | Protein_13 | conjugal transfer protein | - |
| J1790_RS00080 | 13060..14061 | - | 1002 | WP_206606733 | bifunctional lysozyme/C40 family peptidase | orf14 |
| J1790_RS00085 | 14058..16235 | - | 2178 | WP_001574271 | YtxH domain-containing protein | orf15 |
| J1790_RS00090 | 16238..18685 | - | 2448 | WP_000331160 | ATP-binding protein | virb4 |
| J1790_RS00095 | 18669..19061 | - | 393 | WP_000723888 | conjugal transfer protein | orf17a |
| J1790_RS00100 | 19150..19647 | - | 498 | WP_000342539 | antirestriction protein ArdA | - |
| J1790_RS00105 | 19764..19985 | - | 222 | WP_001009056 | hypothetical protein | orf19 |
| J1790_RS00110 | 20028..21233 | - | 1206 | WP_000398284 | MobT family relaxase | - |
| J1790_RS00115 | 21256..21408 | - | 153 | WP_031764745 | hypothetical protein | - |
| J1790_RS00120 | 21411..22796 | - | 1386 | WP_000813488 | FtsK/SpoIIIE domain-containing protein | virb4 |
| J1790_RS00125 | 22825..23211 | - | 387 | WP_000985015 | YdcP family protein | orf23 |
| J1790_RS00130 | 23227..23541 | - | 315 | WP_000420682 | YdcP family protein | orf23 |
| J1790_RS00135 | 24102..24791 | - | 690 | Protein_25 | IS256-like element ISEf1 family transposase | - |
Host bacterium
| ID | 3819 | GenBank | NZ_MT683616 |
| Plasmid name | pEh-GE2 | Incompatibility group | - |
| Plasmid size | 24793 bp | Coordinate of oriT [Strand] | 21233..21436 [-] |
| Host baterium | Enterococcus hirae strain GE2 |
Cargo genes
| Drug resistance gene | fexB, poxtA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |