Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103352
Name   oriT_pESBL26 in_silico
Organism   Escherichia coli strain E. coli UB-ESBL26
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT230257 (42455..42543 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pESBL26
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2509 GenBank   WP_024143648
Name   nikB_G6836_RS00200_pESBL26 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   1076 GenBank   WP_001283947
Name   WP_001283947_pESBL26 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2283 GenBank   WP_001289276
Name   trbC_G6836_RS00195_pESBL26 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 7648..37077

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6836_RS00025 (G6836_00003) 4049..4924 + 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
G6836_RS00030 (G6836_00004) 4971..5315 - 345 Protein_5 WbuC family cupin fold metalloprotein -
G6836_RS00630 5315..5524 + 210 Protein_6 hypothetical protein -
G6836_RS00635 5875..6030 + 156 WP_001302705 hypothetical protein -
G6836_RS00035 6027..6344 - 318 WP_001558195 hypothetical protein -
G6836_RS00040 (G6836_00005) 6343..7497 + 1155 WP_001139957 site-specific integrase -
G6836_RS00045 (G6836_00006) 7648..8472 + 825 WP_001238939 conjugal transfer protein TraE traE
G6836_RS00050 (G6836_00007) 8557..9759 + 1203 WP_000976351 conjugal transfer protein TraF -
G6836_RS00055 (G6836_00008) 9819..10403 + 585 WP_000977522 histidine phosphatase family protein -
G6836_RS00060 (G6836_00009) 10798..11256 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
G6836_RS00065 (G6836_00010) 11253..12071 + 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
G6836_RS00070 (G6836_00011) 12068..13216 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
G6836_RS00075 (G6836_00012) 13213..13503 + 291 WP_001299214 hypothetical protein traK
G6836_RS00080 (G6836_00013) 13518..14069 + 552 WP_000014583 phospholipase D family protein -
G6836_RS00085 (G6836_00014) 14158..17925 + 3768 WP_001141527 LPD7 domain-containing protein -
G6836_RS00090 (G6836_00015) 17943..18290 + 348 WP_001055900 conjugal transfer protein traL
G6836_RS00095 (G6836_00016) 18287..18979 + 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
G6836_RS00100 (G6836_00017) 18990..19973 + 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
G6836_RS00105 (G6836_00018) 19976..21265 + 1290 WP_001272003 conjugal transfer protein TraO traO
G6836_RS00110 (G6836_00019) 21265..21969 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
G6836_RS00115 (G6836_00020) 21969..22496 + 528 WP_001055569 conjugal transfer protein TraQ traQ
G6836_RS00120 (G6836_00021) 22547..22951 + 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
G6836_RS00125 (G6836_00022) 23015..23203 + 189 WP_001277255 putative conjugal transfer protein TraS -
G6836_RS00130 (G6836_00023) 23187..23987 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
G6836_RS00135 (G6836_00024) 24077..27121 + 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
G6836_RS00140 (G6836_00025) 27121..27735 + 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
G6836_RS00145 (G6836_00026) 27702..28904 + 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
G6836_RS00150 (G6836_00027) 28933..29517 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
G6836_RS00155 (G6836_00028) 29614..31782 + 2169 WP_021537350 DotA/TraY family protein traY
G6836_RS00160 (G6836_00029) 32023..32505 + 483 WP_001672078 plasmid IncI1-type surface exclusion protein ExcA -
G6836_RS00165 (G6836_00030) 32577..32786 - 210 WP_000062603 HEAT repeat domain-containing protein -
G6836_RS00170 33070..33354 + 285 WP_031942457 hypothetical protein -
G6836_RS00655 33419..33514 - 96 WP_001303310 DinQ-like type I toxin DqlB -
G6836_RS00175 (G6836_00032) 34013..34264 + 252 WP_001291964 hypothetical protein -
G6836_RS00180 (G6836_00033) 34336..34488 - 153 WP_001331364 Hok/Gef family protein -
G6836_RS00185 (G6836_00034) 34780..35988 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
G6836_RS00190 (G6836_00035) 36007..37077 + 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
G6836_RS00195 (G6836_00036) 37070..39361 + 2292 WP_001289276 F-type conjugative transfer protein TrbC -

Host bacterium

ID   3795 GenBank   NZ_MT230257
Plasmid name   pESBL26 Incompatibility group   IncI1
Plasmid size   107610 bp Coordinate of oriT [Strand]   42455..42543 [+]
Host baterium   Escherichia coli strain E. coli UB-ESBL26

Cargo genes

Drug resistance gene   blaCTX-M-1, sul2, tet(A)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -