Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103349 |
| Name | oriT_pESBL31  |
| Organism | Escherichia coli strain E. coli UB-ESBL31 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT230271 (15102..15190 [-], 89 nt) |
| oriT length | 89 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 89 nt
>oriT_pESBL31
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2507 | GenBank | WP_001405892 |
| Name | nikB_G6846_RS00135_pESBL31 |
UniProt ID | A0A3V4WX06 |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 103883.32 Da Isoelectric Point: 7.3373
>WP_001405892.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3V4WX06 |
Auxiliary protein
| ID | 1074 | GenBank | WP_001283947 |
| Name | WP_001283947_pESBL31 |
UniProt ID | A0A142CMC2 |
| Length | 110 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12613.57 Da Isoelectric Point: 10.7463
>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142CMC2 |
T4CP
| ID | 2281 | GenBank | WP_001289276 |
| Name | trbC_G6846_RS00140_pESBL31 |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86941.11 Da Isoelectric Point: 6.7713
>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 20568..51495
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G6846_RS00140 (G6846_00026) | 18284..20575 | - | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
| G6846_RS00145 (G6846_00027) | 20568..21638 | - | 1071 | WP_000151582 | IncI1-type conjugal transfer protein TrbB | trbB |
| G6846_RS00150 (G6846_00028) | 21657..22865 | - | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| G6846_RS00155 (G6846_00029) | 23172..23951 | - | 780 | WP_275450201 | protein FinQ | - |
| G6846_RS00160 | 24578..24730 | + | 153 | WP_001303307 | Hok/Gef family protein | - |
| G6846_RS00165 (G6846_00030) | 24802..25053 | - | 252 | WP_001291968 | hypothetical protein | - |
| G6846_RS00170 | 25096..25260 | + | 165 | WP_001323923 | hypothetical protein | - |
| G6846_RS00175 | 25977..26261 | - | 285 | WP_014640317 | hypothetical protein | - |
| G6846_RS00180 (G6846_00032) | 26362..26571 | - | 210 | WP_001140545 | hemolysin expression modulator Hha | - |
| G6846_RS00185 (G6846_00033) | 26669..27283 | - | 615 | WP_000578649 | plasmid IncI1-type surface exclusion protein ExcA | - |
| G6846_RS00190 (G6846_00034) | 27359..29527 | - | 2169 | WP_000698360 | IncI1-type conjugal transfer membrane protein TraY | traY |
| G6846_RS00195 (G6846_00035) | 29624..30208 | - | 585 | WP_001579774 | IncI1-type conjugal transfer protein TraX | - |
| G6846_RS00200 (G6846_00036) | 30237..31439 | - | 1203 | WP_001579773 | IncI1-type conjugal transfer protein TraW | traW |
| G6846_RS00205 (G6846_00037) | 31406..32020 | - | 615 | WP_000337394 | IncI1-type conjugal transfer protein TraV | traV |
| G6846_RS00210 (G6846_00038) | 32020..35064 | - | 3045 | WP_001024752 | IncI1-type conjugal transfer protein TraU | traU |
| G6846_RS00215 (G6846_00039) | 35154..35954 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| G6846_RS00220 (G6846_00040) | 35938..36126 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| G6846_RS00225 (G6846_00041) | 36190..36594 | - | 405 | WP_001579770 | DUF6750 family protein | traR |
| G6846_RS00230 (G6846_00042) | 36645..37172 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| G6846_RS00235 (G6846_00043) | 37172..37876 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| G6846_RS00240 (G6846_00044) | 37876..39165 | - | 1290 | WP_001271997 | conjugal transfer protein TraO | traO |
| G6846_RS00245 (G6846_00045) | 39168..40151 | - | 984 | WP_001579769 | IncI1-type conjugal transfer protein TraN | traN |
| G6846_RS00250 (G6846_00046) | 40162..40854 | - | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| G6846_RS00255 (G6846_00047) | 40851..41198 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| G6846_RS00260 (G6846_00048) | 41216..44983 | - | 3768 | WP_001579768 | LPD7 domain-containing protein | - |
| G6846_RS00265 (G6846_00049) | 45073..45624 | - | 552 | WP_000014584 | phospholipase D family protein | - |
| G6846_RS00270 (G6846_00050) | 45639..45929 | - | 291 | WP_001372180 | hypothetical protein | traK |
| G6846_RS00275 (G6846_00051) | 45926..47074 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| G6846_RS00280 (G6846_00052) | 47071..47889 | - | 819 | WP_000646097 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| G6846_RS00285 (G6846_00053) | 47886..48344 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| G6846_RS00290 (G6846_00054) | 48739..49323 | - | 585 | WP_001579766 | histidine phosphatase family protein | - |
| G6846_RS00295 (G6846_00055) | 49383..50585 | - | 1203 | WP_000976353 | conjugal transfer protein TraF | - |
| G6846_RS00300 (G6846_00056) | 50671..51495 | - | 825 | WP_001238929 | conjugal transfer protein TraE | traE |
| G6846_RS00305 (G6846_00057) | 51646..52800 | - | 1155 | WP_001139958 | site-specific integrase | - |
| G6846_RS00310 | 53113..53268 | - | 156 | WP_001302705 | hypothetical protein | - |
| G6846_RS00330 | 53797..54186 | + | 390 | WP_223873413 | shufflon protein D' | - |
| G6846_RS00340 | 54216..54437 | + | 222 | Protein_63 | prepilin | - |
| G6846_RS00335 | 54434..54877 | - | 444 | WP_077253131 | prepilin, shufflon protein A | - |
Host bacterium
| ID | 3792 | GenBank | NZ_MT230271 |
| Plasmid name | pESBL31 | Incompatibility group | - |
| Plasmid size | 54918 bp | Coordinate of oriT [Strand] | 15102..15190 [-] |
| Host baterium | Escherichia coli strain E. coli UB-ESBL31 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |