Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103325
• Name: oriT1_nh1|unnamed
• Organism: UNVERIFIED: Listeria monocytogenes strain nh1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MH277333 (57763..57786 [+], 24 nt)
• oriT length: 24 nt
• IRs (inverted repeats): _
• Location of nic site: 10..11
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 24 nt

>oriT1_nh1|unnamed
GTCTAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobL_H5B54_RS00455_nh1|unnamed

• ID: 2483
• GenBank: WP_181711143
• Name: MobL_H5B54_RS00455_nh1|unnamed
• UniProt ID: _
• Length: 446 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 446 a.a.
• Molecular weight: 52358.18 Da
• Isoelectric Point: 9.8980

>WP_181711143.1 MULTISPECIES: MobP2 family relaxase [Listeria]
MSKITAGIVLKTKFIVPNSPLNKSKQYWPNYIKYIDRDEAVRNAHYKEFSLIGNEEERAEEDKERNKKYI
DYMGNPQKTSGLFTEGKTRLTAAEKKKYKSAFQTAQDNNSVMFQHVISFDNDWLAKQGIYDPSIGMLDEK
RLQQVTSATMKSFLRKEEMDGSAIWLAAIHKNTKHFHVHISVTEPTPTRKFYSNKKRPELEKGTDGKPVR
DGRMAPATRRAVRSTVYSEIAQPGVFYQRIDDYAKAQLLTPLRESKEALRSEENVALFFELHQMYKAHSG
QWNYGNLNKHPAKKVVDQITTNYLMQHHPEEMQVFRKAWEHEGKLQQEAYGEKTEFNIHTYLESKDEKLY
KQLGNQVLQEVKKYDRSNGESKASGHYLDQQGNLKVTGRLTPKDKLERYRRRRGSVIYQLKKMDYELDNS
YKKWKNDRAFEELEQEMEREKSVHRG

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (5-372)

Protein structure

No available structure.

Host bacterium

• ID: 3768
• GenBank: NZ_MH277333
• Plasmid name: nh1|unnamed
• Incompatibility group: -
• Plasmid size: 85554 bp
• Coordinate of oriT [Strand]: 57763..57786 [+]; 59177..59202 [+]
• Host baterium: UNVERIFIED: Listeria monocytogenes strain nh1

Cargo genes

• Drug resistance gene: dfrG, tet(S), lnu(B), lsa(E), ant(6)-Ia, erm(B), aph(3')-III, cat
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9