Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103324
Name   oriT_pKRA-GES-5 in_silico
Organism   Klebsiella pneumoniae strain NMI4661/17
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MN436715 (22143..22279 [-], 137 nt)
oriT length   137 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pKRA-GES-5
CGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGCCATCCTGTTAAGCTCATCTGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGTGCTTTGCAACAAGCATAAAAGGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2482 GenBank   WP_181715408
Name   Relaxase_H5D87_RS00005_pKRA-GES-5 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101256.80 Da        Isoelectric Point: 9.7696

>WP_181715408.1 TraI/MobA(P) family conjugative relaxase [Klebsiella pneumoniae]
MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNP
VNHYIMSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNG
FDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIE
DGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQ
QVAQREPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWNGKGE
VLNAMRSVIAAEQAAEKAALKEKHKKQREQWRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGA
SGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSF
TVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVT
SIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKLE
RLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHCAPGYENRK
PKHQREDGSYPEVRLLKVERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAY
QRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(17-272)

Large polyvalent protein-associated domain 7 (LPD7)

(438-526)

RepB DNA-primase from phage plasmid (RepB_primase)

(548-806)


  Protein structure



No available structure.




Host bacterium

ID   3767 GenBank   NZ_MN436715
Plasmid name   pKRA-GES-5 Incompatibility group   IncP6
Plasmid size   25010 bp Coordinate of oriT [Strand]   22143..22279 [-]
Host baterium   Klebsiella pneumoniae strain NMI4661/17

Cargo genes

Drug resistance gene   sul1, qacE, blaOXA-10, blaGES-5, dfrB1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -