Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103321
Name   oriT_pEc09 in_silico
Organism   Enterobacter hormaechei strain Ec09
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MH325468 (68942..69092 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      12..17, 24..29  (AACCCG..CGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pEc09
TGGTCGATGTGAACCCGTTTGACCGGGTTATGAATGAGTTGAAAAGTCGTGGCCGCAAGAACGCTCATATCCTGAGCATCCTTCAATTCGACTGGCCCGCATCGGAGGTCATCATCGAGAAGCTGAGCTGTTACATCACAGATGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2479 GenBank   WP_086598692
Name   TraI_2_HTT48_RS00480_pEc09 insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 109442.03 Da        Isoelectric Point: 4.9434

>WP_086598692.1 MobH family relaxase [Enterobacter hormaechei]
MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAYRHGIDRYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE
FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQEDTESTELPKPD
ASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQALKDVGGGLGDIDFPFDVFNAS
AETACTDATNLEIPDITEPEKQEEQPKPDFVPQEQNSLQNGDFPIFSDSDEPPSWAIEPLPMLTDTPEQT
IPAPDMQHAGKPKELEKDAKTLLSEMLAGYGEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGA
RSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELV
SPPGSGVSKNKSAPKQQSRKKTQQQKPEVDAGAGSEQKTKDKSLQRQPKEKQGNVASLVEEQKRKPVHDQ
EKQNVARLPKREAQPVAPKPKVEHERELGHVEVRERDEPEVREFEPPKAKTNPKDINEEDFLPSGVTPEK
ALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHILYGLLSRAQRRPLLKKRQGKL
YLEVDKA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   2264 GenBank   WP_086598693
Name   traD_HTT48_RS00485_pEc09 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69234.73 Da        Isoelectric Point: 7.1093

>WP_086598693.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacter cloacae complex]
MTMSYDPLAYEMPWRPNYEKNAVAGWMAASCAALAVEQVSTMPSEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVLTHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVKPDWEAEAGAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNTDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-293)

  Protein structure



No available structure.



ID   2265 GenBank   WP_012766329
Name   traC_HTT48_RS00560_pEc09 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92529.82 Da        Isoelectric Point: 5.8328

>WP_012766329.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIITIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDQSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTSLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRILAKTSKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQYLAQIRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDREAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYDDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSQKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRKQAA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 67026..92130

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTT48_RS00470 63066..63299 - 234 WP_001191893 hypothetical protein -
HTT48_RS00475 63281..63898 - 618 WP_012766323 hypothetical protein -
HTT48_RS00480 64066..67029 + 2964 WP_086598692 MobH family relaxase -
HTT48_RS00485 67026..68891 + 1866 WP_086598693 conjugative transfer system coupling protein TraD virb4
HTT48_RS00490 68902..69486 + 585 WP_000332867 hypothetical protein -
HTT48_RS00495 69443..70072 + 630 WP_000743448 DUF4400 domain-containing protein tfc7
HTT48_RS00500 70083..70529 + 447 WP_086626436 hypothetical protein -
HTT48_RS00505 70539..70916 + 378 WP_000869260 hypothetical protein -
HTT48_RS00510 70916..71578 + 663 WP_000045163 hypothetical protein -
HTT48_RS00515 71913..72260 + 348 WP_001102160 hypothetical protein -
HTT48_RS00520 72403..72684 + 282 WP_000805626 type IV conjugative transfer system protein TraL traL
HTT48_RS00525 72681..73307 + 627 WP_001049718 TraE/TraK family type IV conjugative transfer system protein traE
HTT48_RS00530 73291..74208 + 918 WP_012766326 type-F conjugative transfer system secretin TraK traK
HTT48_RS00535 74205..75521 + 1317 WP_000595204 TraB/VirB10 family protein traB
HTT48_RS00540 75518..76096 + 579 WP_000794193 type IV conjugative transfer system lipoprotein TraV traV
HTT48_RS00545 76100..76492 + 393 WP_012766327 TraA family conjugative transfer protein -
HTT48_RS00550 76693..82179 + 5487 WP_086598680 Ig-like domain-containing protein -
HTT48_RS00555 82328..83035 + 708 WP_086598679 DsbC family protein -
HTT48_RS00560 83032..85479 + 2448 WP_012766329 type IV secretion system protein TraC virb4
HTT48_RS00565 85494..85811 + 318 WP_000364398 hypothetical protein -
HTT48_RS00570 85808..86338 + 531 WP_000672456 S26 family signal peptidase -
HTT48_RS00575 86301..87569 + 1269 WP_001553264 TrbC family F-type conjugative pilus assembly protein traW
HTT48_RS00580 87566..88243 + 678 WP_086598678 EAL domain-containing protein -
HTT48_RS00585 88249..89241 + 993 WP_236901774 TraU family protein traU
HTT48_RS00590 89392..92130 + 2739 WP_060446867 conjugal transfer mating pair stabilization protein TraN traN
HTT48_RS00595 92168..93028 - 861 WP_012766333 hypothetical protein -
HTT48_RS00600 93140..93790 - 651 WP_000796641 hypothetical protein -
HTT48_RS00605 94081..94401 + 321 WP_012766334 hypothetical protein -
HTT48_RS00610 94670..94897 + 228 WP_236732500 hypothetical protein -
HTT48_RS00615 95117..96085 + 969 WP_001553257 AAA family ATPase -
HTT48_RS00620 96226..97002 + 777 WP_234805066 hypothetical protein -

Host bacterium

ID   3764 GenBank   NZ_MH325468
Plasmid name   pEc09 Incompatibility group   IncA/C
Plasmid size   152054 bp Coordinate of oriT [Strand]   68942..69092 [+]
Host baterium   Enterobacter hormaechei strain Ec09

Cargo genes

Drug resistance gene   aac(6')-Ib, ant(3'')-Ia, blaOXA-9, blaTEM-1A, sul2, rmtG, blaCTX-M-2, sul1, qacE
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -