Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103315
Name   oriT_p1106-IncFII in_silico
Organism   Escherichia coli strain 1106
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MG825371 (33791..33863 [-], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_p1106-IncFII
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2476 GenBank   WP_058100850
Name   Relaxase_HTT06_RS00240_p1106-IncFII insolico UniProt ID   A0A1M0DNA5
Length   906 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 906 a.a.        Molecular weight: 105098.51 Da        Isoelectric Point: 8.3663

>WP_058100850.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWHSVPKDIFTQVIPGERFRGHSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
ALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPELRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAHIRVQFRDPQLRKLHYHIAEVQRMQALIRLKENVREERLALMAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure


Source ID Structure
AlphaFold DB A0A1M0DNA5


Auxiliary protein

ID   1064 GenBank   WP_001291058
Name   WP_001291058_p1106-IncFII insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   2260 GenBank   WP_058100849
Name   trbC_HTT06_RS00245_p1106-IncFII insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87195.08 Da        Isoelectric Point: 6.6676

>WP_058100849.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFITQQSMILARDLGYRLE
GTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYIAIEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 39449..82574

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTT06_RS00245 (GLDEPIOJ_00048) 37168..39468 - 2301 WP_058100849 F-type conjugative transfer protein TrbC -
HTT06_RS00250 (GLDEPIOJ_00049) 39449..40573 - 1125 WP_113895551 DsbC family protein trbB
HTT06_RS00255 (GLDEPIOJ_00050) 40570..41904 - 1335 WP_172693619 IncI1-type conjugal transfer protein TrbA trbA
HTT06_RS00570 (GLDEPIOJ_00051) 42434..42529 + 96 WP_001443691 DinQ-like type I toxin DqlB -
HTT06_RS00265 (GLDEPIOJ_00052) 42592..42768 - 177 WP_001054907 hypothetical protein -
HTT06_RS00270 (GLDEPIOJ_00053) 42919..43386 - 468 WP_001681654 thermonuclease family protein -
HTT06_RS00275 (GLDEPIOJ_00054) 43550..44173 + 624 WP_072770405 DNA-binding protein -
HTT06_RS00280 (GLDEPIOJ_00055) 44261..44716 + 456 WP_072740804 DUF2569 family protein -
HTT06_RS00285 (GLDEPIOJ_00056) 44916..46451 + 1536 WP_172693627 phosphoethanolamine transferase -
HTT06_RS00290 (GLDEPIOJ_00057) 46544..46786 - 243 WP_000064387 hypothetical protein -
HTT06_RS00295 (GLDEPIOJ_00058) 47120..47272 + 153 WP_001367784 Hok/Gef family protein -
HTT06_RS00300 47269..47430 - 162 WP_021580032 hypothetical protein -
HTT06_RS00305 (GLDEPIOJ_00059) 47586..48230 - 645 WP_172693620 plasmid IncI1-type surface exclusion protein ExcA -
HTT06_RS00310 (GLDEPIOJ_00060) 48317..50482 - 2166 WP_045145407 DotA/TraY family protein traY
HTT06_RS00315 (GLDEPIOJ_00061) 50555..51124 - 570 WP_000650755 conjugal transfer protein TraX -
HTT06_RS00320 (GLDEPIOJ_00062) 51121..52326 - 1206 WP_029391944 conjugal transfer protein TraW traW
HTT06_RS00325 (GLDEPIOJ_00063) 52287..52904 - 618 WP_029391943 hypothetical protein traV
HTT06_RS00330 (GLDEPIOJ_00064) 52904..55948 - 3045 WP_072674850 conjugal transfer protein traU
HTT06_RS00335 (GLDEPIOJ_00065) 56124..56504 - 381 WP_045145405 hypothetical protein -
HTT06_RS00340 (GLDEPIOJ_00066) 56504..56929 - 426 WP_236918923 hypothetical protein -
HTT06_RS00345 (GLDEPIOJ_00067) 56929..57555 - 627 WP_045145401 hypothetical protein traT
HTT06_RS00565 (GLDEPIOJ_00068) 57662..57913 - 252 WP_001277575 hypothetical protein -
HTT06_RS00355 (GLDEPIOJ_00069) 57970..58368 - 399 WP_021532718 DUF6750 family protein traR
HTT06_RS00360 (GLDEPIOJ_00070) 58415..58945 - 531 WP_227316726 conjugal transfer protein TraQ traQ
HTT06_RS00365 (GLDEPIOJ_00071) 58942..59655 - 714 WP_072657236 conjugal transfer protein TraP traP
HTT06_RS00370 (GLDEPIOJ_00072) 59652..60983 - 1332 WP_058100840 conjugal transfer protein TraO traO
HTT06_RS00375 (GLDEPIOJ_00073) 60987..61961 - 975 WP_021532715 DotH/IcmK family type IV secretion protein traN
HTT06_RS00380 (GLDEPIOJ_00074) 61972..62667 - 696 WP_029391932 DotI/IcmL family type IV secretion protein traM
HTT06_RS00385 (GLDEPIOJ_00075) 62679..63029 - 351 WP_021532713 hypothetical protein traL
HTT06_RS00390 (GLDEPIOJ_00076) 63046..67107 - 4062 WP_096986286 LPD7 domain-containing protein -
HTT06_RS00395 (GLDEPIOJ_00077) 67171..67461 - 291 WP_000817799 hypothetical protein traK
HTT06_RS00400 (GLDEPIOJ_00078) 67458..68606 - 1149 WP_072740766 plasmid transfer ATPase TraJ virB11
HTT06_RS00405 (GLDEPIOJ_00079) 68590..69426 - 837 WP_000848138 type IV secretory system conjugative DNA transfer family protein traI
HTT06_RS00410 (GLDEPIOJ_00080) 69423..69875 - 453 WP_001081199 DotD/TraH family lipoprotein -
HTT06_RS00415 (GLDEPIOJ_00082) 70234..71436 - 1203 WP_077250604 conjugal transfer protein TraF -
HTT06_RS00420 (GLDEPIOJ_00083) 71526..72347 - 822 WP_172693621 conjugal transfer protein TraE traE
HTT06_RS00425 (GLDEPIOJ_00084) 72598..73143 - 546 WP_072674828 hypothetical protein -
HTT06_RS00430 (GLDEPIOJ_00085) 73262..73555 - 294 WP_236918924 hypothetical protein -
HTT06_RS00435 (GLDEPIOJ_00086) 73584..74753 - 1170 WP_072674824 site-specific integrase -
HTT06_RS00440 (GLDEPIOJ_00087) 75302..75701 + 400 Protein_84 phage tail protein -
HTT06_RS00550 75706..76191 - 486 WP_236918925 prepilin, shufflon protein A -
HTT06_RS00450 76370..76708 - 339 WP_224732486 shufflon protein B -
HTT06_RS00455 (GLDEPIOJ_00090) 76920..78179 - 1260 WP_172693622 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTT06_RS00460 (GLDEPIOJ_00091) 78176..78883 - 708 WP_153789788 A24 family peptidase -
HTT06_RS00465 (GLDEPIOJ_00092) 78843..79319 - 477 WP_021532704 lytic transglycosylase domain-containing protein virB1
HTT06_RS00470 (GLDEPIOJ_00093) 79378..79914 - 537 WP_001011501 type 4 pilus major pilin -
HTT06_RS00475 (GLDEPIOJ_00094) 79964..81064 - 1101 WP_172693192 type II secretion system F family protein -
HTT06_RS00480 (GLDEPIOJ_00095) 81066..82574 - 1509 WP_100692536 ATPase, T2SS/T4P/T4SS family virB11
HTT06_RS00485 (GLDEPIOJ_00096) 82671..83267 - 597 WP_029391917 hypothetical protein -
HTT06_RS00490 (GLDEPIOJ_00097) 83363..83917 - 555 WP_089581189 hypothetical protein -
HTT06_RS00495 (GLDEPIOJ_00098) 83966..84418 - 453 WP_021547657 type IV pilus biogenesis protein PilP -
HTT06_RS00500 (GLDEPIOJ_00099) 84408..85703 - 1296 WP_172693194 type 4b pilus protein PilO2 -
HTT06_RS00505 (GLDEPIOJ_00100) 85724..87343 - 1620 WP_172693623 PilN family type IVB pilus formation outer membrane protein -

Host bacterium

ID   3758 GenBank   NZ_MG825371
Plasmid name   p1106-IncFII Incompatibility group   IncB/O/K/Z
Plasmid size   92438 bp Coordinate of oriT [Strand]   33791..33863 [-]
Host baterium   Escherichia coli strain 1106

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -