Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103309
Name   oriT_pCESC2 in_silico
Organism   Escherichia coli strain 24-S11
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MF156266 (50063..50167 [+], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      32..37, 40..45  (GCCGGC..GCCGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 105 nt

>oriT_pCESC2
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2471 GenBank   WP_047731900
Name   Relaxase_HTN87_RS00370_pCESC2 insolico UniProt ID   A0A2L1F4H9
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75191.01 Da        Isoelectric Point: 10.0563

>WP_047731900.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAALEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSKSDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A2L1F4H9


T4CP

ID   2251 GenBank   WP_004206885
Name   t4cp2_HTN87_RS00070_pCESC2 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52965..72203

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTN87_RS00350 48049..48360 + 312 WP_004187333 hypothetical protein -
HTN87_RS00355 48493..49029 + 537 WP_004206920 hypothetical protein -
HTN87_RS00360 49530..49895 - 366 WP_015586046 hypothetical protein -
HTN87_RS00365 50171..50488 + 318 WP_004206923 plasmid mobilization protein MobA -
HTN87_RS00370 50475..52454 + 1980 WP_047731900 TraI/MobA(P) family conjugative relaxase -
HTN87_RS00375 52468..52968 + 501 WP_004187320 DotD/TraH family lipoprotein -
HTN87_RS00380 52965..53744 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
HTN87_RS00385 53755..54918 + 1164 WP_094009851 plasmid transfer ATPase TraJ virB11
HTN87_RS00390 54908..55168 + 261 WP_004187310 IcmT/TraK family protein traK
HTN87_RS00480 55193..58540 + 3348 WP_225995901 LPD7 domain-containing protein -
HTN87_RS00400 58464..59018 + 555 WP_011154467 hypothetical protein traL
HTN87_RS00405 59019..59216 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
HTN87_RS00410 59203..59613 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HTN87_RS00415 59612..60394 + 783 WP_004206929 DotI/IcmL family type IV secretion protein traM
HTN87_RS00420 60403..61554 + 1152 WP_074189215 DotH/IcmK family type IV secretion protein traN
HTN87_RS00425 61566..62915 + 1350 WP_047731905 conjugal transfer protein TraO traO
HTN87_RS00430 62927..63631 + 705 WP_047731908 conjugal transfer protein TraP traP
HTN87_RS00435 63655..64185 + 531 WP_011091077 conjugal transfer protein TraQ traQ
HTN87_RS00440 64202..64591 + 390 WP_004187479 DUF6750 family protein traR
HTN87_RS00445 64637..65131 + 495 WP_004187480 hypothetical protein -
HTN87_RS00450 65128..68178 + 3051 WP_064424658 hypothetical protein traU
HTN87_RS00455 68175..69383 + 1209 WP_004187486 conjugal transfer protein TraW traW
HTN87_RS00460 69380..70030 + 651 WP_004187488 hypothetical protein -
HTN87_RS00465 70023..72203 + 2181 WP_004187492 DotA/TraY family protein traY
HTN87_RS00470 72206..72859 + 654 WP_004206935 hypothetical protein -

Host bacterium

ID   3752 GenBank   NZ_MF156266
Plasmid name   pCESC2 Incompatibility group   IncL/M
Plasmid size   72932 bp Coordinate of oriT [Strand]   50063..50167 [+]
Host baterium   Escherichia coli strain 24-S11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   arsR, arsD, arsA, arsB, arsC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -