Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103305 |
| Name | oriT_pKP91  |
| Organism | Klebsiella pneumoniae strain KP91 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MG736312 (10753..10802 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
TGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pKP91
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2245 | GenBank | WP_228303505 |
| Name | traD_HTQ44_RS00455_pKP91 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85885.99 Da Isoelectric Point: 5.0596
>WP_228303505.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVIAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVIAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 79225..95796
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTQ44_RS00455 (pKP91-00092) | 79225..81537 | - | 2313 | WP_228303505 | type IV conjugative transfer system coupling protein TraD | virb4 |
| HTQ44_RS00460 (pKP91-00093) | 81664..82353 | - | 690 | WP_172691968 | hypothetical protein | - |
| HTQ44_RS00465 (pKP91-00094) | 82546..83277 | - | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| HTQ44_RS00470 (pKP91-00095) | 83466..83993 | - | 528 | WP_040120388 | conjugal transfer protein TraS | - |
| HTQ44_RS00475 (pKP91-00096) | 83999..86848 | - | 2850 | WP_172691976 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HTQ44_RS00480 | 86858..88217 | - | 1360 | Protein_97 | conjugal transfer pilus assembly protein TraH | - |
| HTQ44_RS00485 (pKP91-00098) | 88207..88653 | - | 447 | WP_172691970 | conjugal transfer protein TrbF | - |
| HTQ44_RS00490 (pKP91-00099) | 88700..89257 | - | 558 | WP_172691971 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HTQ44_RS00495 (pKP91-00100) | 89229..89468 | - | 240 | WP_009309875 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HTQ44_RS00500 (pKP91-00101) | 89479..90231 | - | 753 | WP_172691972 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HTQ44_RS00505 (pKP91-00102) | 90252..90578 | - | 327 | WP_016529362 | hypothetical protein | - |
| HTQ44_RS00570 (pKP91-00103) | 90624..90809 | - | 186 | WP_042922350 | hypothetical protein | - |
| HTQ44_RS00510 (pKP91-00104) | 90806..91042 | - | 237 | WP_114499720 | conjugal transfer protein TrbE | - |
| HTQ44_RS00515 (pKP91-00105) | 91074..93029 | - | 1956 | WP_172691973 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HTQ44_RS00520 (pKP91-00106) | 93088..93726 | - | 639 | WP_015065635 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HTQ44_RS00525 (pKP91-00107) | 93739..94728 | - | 990 | WP_032441886 | conjugal transfer pilus assembly protein TraU | traU |
| HTQ44_RS00530 (pKP91-00108) | 94725..95126 | - | 402 | WP_004194979 | hypothetical protein | - |
| HTQ44_RS00535 (pKP91-00109) | 95161..95796 | - | 636 | WP_020325113 | type-F conjugative transfer system protein TraW | traW |
Host bacterium
| ID | 3748 | GenBank | NZ_MG736312 |
| Plasmid name | pKP91 | Incompatibility group | IncFIA |
| Plasmid size | 95983 bp | Coordinate of oriT [Strand] | 10753..10802 [+] |
| Host baterium | Klebsiella pneumoniae strain KP91 |
Cargo genes
| Drug resistance gene | mcr-8 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |