Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103292 |
| Name | oriT_pTA9  |
| Organism | Escherichia coli strain TA9 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MN106912 (26994..27046 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pTA9
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTCTCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTCTCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2235 | GenBank | WP_015059539 |
| Name | t4cp2_H5D35_RS00375_pTA9 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 48393..65736
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H5D35_RS00305 | 43798..44922 | - | 1125 | WP_000486716 | site-specific integrase | - |
| H5D35_RS00465 | 45063..45284 | + | 222 | Protein_63 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| H5D35_RS00315 | 45910..46170 | - | 261 | WP_029237379 | hypothetical protein | - |
| H5D35_RS00320 | 46289..46444 | + | 156 | WP_001358489 | hypothetical protein | - |
| H5D35_RS00325 | 46441..47741 | - | 1301 | Protein_66 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| H5D35_RS00330 | 47754..48389 | - | 636 | WP_000934977 | A24 family peptidase | - |
| H5D35_RS00335 | 48393..48875 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| H5D35_RS00340 | 48941..49498 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| H5D35_RS00345 | 49543..50652 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| H5D35_RS00350 | 50643..52181 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| H5D35_RS00355 | 52206..52700 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| H5D35_RS00360 | 52684..54006 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| H5D35_RS00365 | 54045..55688 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| H5D35_RS00370 | 55681..56217 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| H5D35_RS00375 | 56264..58222 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| H5D35_RS00380 | 58238..59293 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| H5D35_RS00385 | 59312..60451 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| H5D35_RS00390 | 60441..61142 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| H5D35_RS00395 | 61208..61942 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| H5D35_RS00400 | 62108..64465 | - | 2358 | WP_063078682 | VirB4 family type IV secretion system protein | virb4 |
| H5D35_RS00405 | 64471..64791 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| H5D35_RS00460 | 64862..65152 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| H5D35_RS00415 | 65152..65736 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| H5D35_RS00420 | 65757..66155 | - | 399 | WP_001153665 | hypothetical protein | - |
| H5D35_RS00425 | 66274..66594 | - | 321 | WP_257792849 | type IV pilus biogenesis protein PilM | - |
Host bacterium
| ID | 3735 | GenBank | NZ_MN106912 |
| Plasmid name | pTA9 | Incompatibility group | IncI2 |
| Plasmid size | 66603 bp | Coordinate of oriT [Strand] | 26994..27046 [-] |
| Host baterium | Escherichia coli strain TA9 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |