Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103291
Name   oriT_p15078279 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK181566 (52191..52279 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p15078279
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2460 GenBank   WP_025492051
Name   nikB_HTW76_RS00355_p15078279 insolico UniProt ID   A0A075UR71
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104022.47 Da        Isoelectric Point: 7.4265

>WP_025492051.1 IncI1-type relaxase NikB [Escherichia coli]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYAIRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A075UR71


Auxiliary protein

ID   1058 GenBank   WP_001283947
Name   WP_001283947_p15078279 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2234 GenBank   WP_001289276
Name   trbC_HTW76_RS00360_p15078279 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 57657..98459

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTW76_RS00360 (CV83906_1p096) 55373..57664 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTW76_RS00365 (CV83906_1p097) 57657..58727 - 1071 WP_000151582 IncI1-type conjugal transfer protein TrbB trbB
HTW76_RS00370 (CV83906_1p098) 58746..59954 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTW76_RS00375 (CV83906_1p099) 60261..61040 - 780 WP_275450201 protein FinQ -
HTW76_RS00380 (CV83906_1p100) 61667..61819 + 153 WP_001303307 Hok/Gef family protein -
HTW76_RS00385 (CV83906_1p101) 61891..62142 - 252 WP_001291965 hypothetical protein -
HTW76_RS00390 (CV83906_1p104) 63065..63349 - 285 WP_014640317 hypothetical protein -
HTW76_RS00395 63450..63659 - 210 Protein_75 hemolysin expression modulator Hha -
HTW76_RS00400 (CV83906_1p106) 63757..64371 - 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
HTW76_RS00405 (CV83906_1p107) 64447..66615 - 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
HTW76_RS00410 (CV83906_1p108) 66712..67296 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTW76_RS00415 (CV83906_1p109) 67325..68527 - 1203 WP_001189159 IncI1-type conjugal transfer protein TraW traW
HTW76_RS00420 (CV83906_1p110) 68494..69108 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
HTW76_RS00425 (CV83906_1p111) 69108..72152 - 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
HTW76_RS00430 (CV83906_1p112) 72242..73042 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTW76_RS00435 (CV83906_1p113) 73026..73214 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTW76_RS00440 (CV83906_1p114) 73278..73682 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
HTW76_RS00445 (CV83906_1p115) 73733..74260 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTW76_RS00450 (CV83906_1p116) 74260..74964 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTW76_RS00455 (CV83906_1p117) 74964..76253 - 1290 WP_001271997 conjugal transfer protein TraO traO
HTW76_RS00460 (CV83906_1p118) 76256..77239 - 984 WP_134316973 IncI1-type conjugal transfer protein TraN traN
HTW76_RS00465 (CV83906_1p119) 77250..77942 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
HTW76_RS00470 (CV83906_1p120) 77939..78286 - 348 WP_001055900 conjugal transfer protein traL
HTW76_RS00475 (CV83906_1p121) 78304..82071 - 3768 WP_001141539 LPD7 domain-containing protein -
HTW76_RS00480 (CV83906_1p122) 82161..82712 - 552 WP_000014584 phospholipase D family protein -
HTW76_RS00485 (CV83906_1p123) 82727..83017 - 291 WP_001299214 hypothetical protein traK
HTW76_RS00490 (CV83906_1p124) 83014..84162 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTW76_RS00495 (CV83906_1p125) 84159..84977 - 819 WP_025492003 IncI1-type conjugal transfer lipoprotein TraI traI
HTW76_RS00500 (CV83906_1p126) 84974..85432 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTW76_RS00505 (CV83906_1p128) 85827..86411 - 585 WP_000977522 histidine phosphatase family protein -
HTW76_RS00510 (CV83906_1p129) 86471..87673 - 1203 WP_000976349 conjugal transfer protein TraF -
HTW76_RS00515 (CV83906_1p130) 87759..88583 - 825 WP_001238927 conjugal transfer protein TraE traE
HTW76_RS00520 (CV83906_1p131) 88734..89852 - 1119 WP_236918811 site-specific integrase -
HTW76_RS00525 89863..91091 + 1229 WP_089541817 IS3 family transposase -
HTW76_RS00645 91102..91224 - 123 Protein_102 integrase -
HTW76_RS00650 91208..91507 + 300 WP_236918812 pilus assembly protein PilV -
HTW76_RS00655 91781..92035 + 255 Protein_104 hypothetical protein -
HTW76_RS00535 92661..93953 - 1293 WP_024198499 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTW76_RS00540 93953..94609 - 657 WP_001389386 prepilin peptidase -
HTW76_RS00545 94594..95154 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTW76_RS00550 95164..95778 - 615 WP_000959786 type 4 pilus major pilin -
HTW76_RS00555 95796..96893 - 1098 WP_001208805 type II secretion system F family protein -
HTW76_RS00560 96906..98459 - 1554 WP_025492055 ATPase, T2SS/T4P/T4SS family virB11
HTW76_RS00565 98470..98922 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
HTW76_RS00570 98909..100204 - 1296 WP_000752772 type 4b pilus protein PilO2 -
HTW76_RS00575 100197..101879 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTW76_RS00580 101893..102330 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTW76_RS00585 102330..103397 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3734 GenBank   NZ_MK181566
Plasmid name   p15078279 Incompatibility group   IncI1
Plasmid size   111403 bp Coordinate of oriT [Strand]   52191..52279 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   sul2, blaCTX-M-1, sul1, qacE, ant(3'')-Ia, dfrA1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2