Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103289
Name   oriT_pESBL20150178 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK181568 (51532..51620 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pESBL20150178
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2458 GenBank   WP_024143648
Name   nikB_HTV41_RS00340_pESBL20150178 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   1056 GenBank   WP_001283947
Name   WP_001283947_pESBL20150178 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2232 GenBank   WP_001289276
Name   trbC_HTV41_RS00345_pESBL20150178 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56998..97976

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTV41_RS00345 54714..57005 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTV41_RS00350 56998..58068 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTV41_RS00355 58087..59295 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTV41_RS00360 59587..59739 + 153 WP_001331364 Hok/Gef family protein -
HTV41_RS00365 59811..60062 - 252 WP_001291964 hypothetical protein -
HTV41_RS00630 60561..60656 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HTV41_RS00370 60721..60897 - 177 WP_001054904 hypothetical protein -
HTV41_RS00375 61289..61498 + 210 WP_000062603 HEAT repeat domain-containing protein -
HTV41_RS00380 61570..62244 - 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTV41_RS00385 62294..64462 - 2169 WP_021537350 DotA/TraY family protein traY
HTV41_RS00390 64559..65143 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTV41_RS00395 65172..66374 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HTV41_RS00400 66341..66955 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HTV41_RS00405 66955..69999 - 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
HTV41_RS00410 70089..70889 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTV41_RS00415 70873..71061 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTV41_RS00420 71125..71529 - 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
HTV41_RS00425 71580..72107 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTV41_RS00430 72107..72811 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTV41_RS00435 72811..74100 - 1290 WP_001272003 conjugal transfer protein TraO traO
HTV41_RS00440 74103..75086 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HTV41_RS00445 75097..75789 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTV41_RS00450 75786..76133 - 348 WP_001055900 conjugal transfer protein traL
HTV41_RS00455 76151..79918 - 3768 WP_001141527 LPD7 domain-containing protein -
HTV41_RS00460 80007..80558 - 552 WP_000014583 phospholipase D family protein -
HTV41_RS00465 80573..80863 - 291 WP_001299214 hypothetical protein traK
HTV41_RS00470 80860..82008 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTV41_RS00475 82005..82823 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HTV41_RS00480 82820..83278 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTV41_RS00485 83673..84257 - 585 WP_000977522 histidine phosphatase family protein -
HTV41_RS00490 84317..85519 - 1203 WP_000976351 conjugal transfer protein TraF -
HTV41_RS00495 85604..86428 - 825 WP_001238939 conjugal transfer protein TraE traE
HTV41_RS00500 86579..87733 - 1155 WP_001139957 site-specific integrase -
HTV41_RS00505 88027..89289 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HTV41_RS00510 89577..90452 + 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
HTV41_RS00515 90499..90843 - 345 Protein_101 WbuC family cupin fold metalloprotein -
HTV41_RS00625 90843..91052 + 210 Protein_102 hypothetical protein -
HTV41_RS00520 91933..92181 + 249 WP_001349157 hypothetical protein -
HTV41_RS00525 92178..93470 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTV41_RS00530 93470..94126 - 657 WP_001193553 prepilin peptidase -
HTV41_RS00535 94111..94671 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTV41_RS00540 94681..95295 - 615 WP_000959785 type 4 pilus major pilin -
HTV41_RS00545 95313..96410 - 1098 WP_001208805 type II secretion system F family protein -
HTV41_RS00550 96423..97976 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HTV41_RS00555 97987..98439 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
HTV41_RS00560 98426..99721 - 1296 WP_000752772 type 4b pilus protein PilO2 -
HTV41_RS00565 99714..101396 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTV41_RS00570 101410..101847 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTV41_RS00575 101847..102914 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3732 GenBank   NZ_MK181568
Plasmid name   pESBL20150178 Incompatibility group   IncI1
Plasmid size   108457 bp Coordinate of oriT [Strand]   51532..51620 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   dfrA17, aadA5, sul2, blaCTX-M-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -