Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103286
Name   oriT_pESBL20150097 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK181567 (51550..51638 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pESBL20150097
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2455 GenBank   WP_024143648
Name   nikB_HTY30_RS00350_pESBL20150097 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   1053 GenBank   WP_001283947
Name   WP_001283947_pESBL20150097 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2229 GenBank   WP_001289276
Name   trbC_HTY30_RS00355_pESBL20150097 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 57016..97994

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTY30_RS00355 54732..57023 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTY30_RS00360 57016..58086 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTY30_RS00365 58105..59313 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTY30_RS00370 59605..59757 + 153 WP_001331364 Hok/Gef family protein -
HTY30_RS00375 59829..60080 - 252 WP_001291964 hypothetical protein -
HTY30_RS00635 60579..60674 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HTY30_RS00380 60739..61023 - 285 WP_031942457 hypothetical protein -
HTY30_RS00385 61307..61516 + 210 WP_000062603 HEAT repeat domain-containing protein -
HTY30_RS00390 61588..62262 - 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTY30_RS00395 62312..64480 - 2169 WP_021537350 DotA/TraY family protein traY
HTY30_RS00400 64577..65161 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTY30_RS00405 65190..66392 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HTY30_RS00410 66359..66973 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HTY30_RS00415 66973..70017 - 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
HTY30_RS00420 70107..70907 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTY30_RS00425 70891..71079 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTY30_RS00430 71143..71547 - 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
HTY30_RS00435 71598..72125 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTY30_RS00440 72125..72829 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTY30_RS00445 72829..74118 - 1290 WP_001272003 conjugal transfer protein TraO traO
HTY30_RS00450 74121..75104 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HTY30_RS00455 75115..75807 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTY30_RS00460 75804..76151 - 348 WP_001055900 conjugal transfer protein traL
HTY30_RS00465 76169..79936 - 3768 WP_001141527 LPD7 domain-containing protein -
HTY30_RS00470 80025..80576 - 552 WP_000014583 phospholipase D family protein -
HTY30_RS00475 80591..80881 - 291 WP_001299214 hypothetical protein traK
HTY30_RS00480 80878..82026 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTY30_RS00485 82023..82841 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HTY30_RS00490 82838..83296 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTY30_RS00495 83691..84275 - 585 WP_000977522 histidine phosphatase family protein -
HTY30_RS00500 84335..85537 - 1203 WP_000976351 conjugal transfer protein TraF -
HTY30_RS00505 85622..86446 - 825 WP_001238939 conjugal transfer protein TraE traE
HTY30_RS00510 86597..87751 - 1155 WP_001139957 site-specific integrase -
HTY30_RS00515 88045..89307 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HTY30_RS00520 89595..90470 + 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
HTY30_RS00525 90517..90861 - 345 Protein_104 WbuC family cupin fold metalloprotein -
HTY30_RS00630 90861..91070 + 210 Protein_105 hypothetical protein -
HTY30_RS00655 91846..92067 + 222 Protein_106 prepilin -
HTY30_RS00530 92064..93488 - 1425 WP_001389385 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTY30_RS00535 93488..94144 - 657 WP_001193553 prepilin peptidase -
HTY30_RS00540 94129..94689 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTY30_RS00545 94699..95313 - 615 WP_000959785 type 4 pilus major pilin -
HTY30_RS00550 95331..96428 - 1098 WP_001208805 type II secretion system F family protein -
HTY30_RS00555 96441..97994 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HTY30_RS00560 98005..98457 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
HTY30_RS00565 98444..99739 - 1296 WP_000752772 type 4b pilus protein PilO2 -
HTY30_RS00570 99732..101414 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTY30_RS00575 101428..101865 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTY30_RS00580 101865..102932 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3729 GenBank   NZ_MK181567
Plasmid name   pESBL20150097 Incompatibility group   IncI1
Plasmid size   107761 bp Coordinate of oriT [Strand]   51550..51638 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   aadA5, sul2, blaCTX-M-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -