Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103285
Name   oriT_p199 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK181559 (63151..63239 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p199
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2454 GenBank   WP_024143648
Name   nikB_HTV44_RS00395_p199 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   1052 GenBank   WP_001283947
Name   WP_001283947_p199 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2228 GenBank   WP_001289276
Name   trbC_HTV44_RS00400_p199 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 68617..112135

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTV44_RS00400 66333..68624 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTV44_RS00405 68617..69687 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTV44_RS00410 69706..70914 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTV44_RS00415 71206..71358 + 153 WP_001331364 Hok/Gef family protein -
HTV44_RS00420 71430..71681 - 252 WP_001291964 hypothetical protein -
HTV44_RS00710 72180..72275 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HTV44_RS00690 72340..72516 - 177 WP_001054904 hypothetical protein -
HTV44_RS00430 72908..73117 + 210 WP_000062603 HEAT repeat domain-containing protein -
HTV44_RS00435 73189..73863 - 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTV44_RS00440 73913..76081 - 2169 WP_021537350 DotA/TraY family protein traY
HTV44_RS00445 76178..76762 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTV44_RS00450 76791..77993 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HTV44_RS00455 77960..78574 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HTV44_RS00460 78574..81618 - 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
HTV44_RS00465 81708..82508 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTV44_RS00470 82492..82680 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTV44_RS00475 82744..83148 - 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
HTV44_RS00480 83199..83726 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTV44_RS00485 83726..84430 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTV44_RS00490 84430..85719 - 1290 WP_001272003 conjugal transfer protein TraO traO
HTV44_RS00495 85722..86705 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HTV44_RS00500 86716..87408 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTV44_RS00505 87405..87752 - 348 WP_001055900 conjugal transfer protein traL
HTV44_RS00510 87770..91537 - 3768 WP_001141527 LPD7 domain-containing protein -
HTV44_RS00515 91626..92177 - 552 WP_000014583 phospholipase D family protein -
HTV44_RS00520 92192..92482 - 291 WP_001299214 hypothetical protein traK
HTV44_RS00525 92479..93042 - 564 Protein_103 ATPase, T2SS/T4P/T4SS family -
HTV44_RS00530 93130..93555 + 426 WP_000422741 transposase -
HTV44_RS00535 93552..93902 + 351 WP_000624722 IS66 family insertion sequence element accessory protein TnpB -
HTV44_RS00540 93933..95546 + 1614 WP_000080195 IS66-like element ISEc23 family transposase -
HTV44_RS00545 95577..96167 - 591 Protein_107 plasmid transfer ATPase TraJ -
HTV44_RS00550 96164..96982 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HTV44_RS00555 96979..97437 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTV44_RS00560 97832..98416 - 585 WP_000977522 histidine phosphatase family protein -
HTV44_RS00565 98476..99678 - 1203 WP_000976351 conjugal transfer protein TraF -
HTV44_RS00570 99763..100587 - 825 WP_001238939 conjugal transfer protein TraE traE
HTV44_RS00575 100738..101892 - 1155 WP_001139957 site-specific integrase -
HTV44_RS00580 102186..103448 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HTV44_RS00585 103736..104611 + 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
HTV44_RS00590 104658..105002 - 345 Protein_116 WbuC family cupin fold metalloprotein -
HTV44_RS00705 105002..105211 + 210 Protein_117 hypothetical protein -
HTV44_RS00725 105465..105707 + 243 Protein_118 prepilin -
HTV44_RS00600 106328..107629 - 1302 WP_001499587 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTV44_RS00605 107629..108285 - 657 WP_001193553 prepilin peptidase -
HTV44_RS00610 108270..108830 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTV44_RS00615 108840..109454 - 615 WP_000959785 type 4 pilus major pilin -
HTV44_RS00620 109472..110569 - 1098 WP_001208805 type II secretion system F family protein -
HTV44_RS00625 110582..112135 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HTV44_RS00630 112146..112598 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
HTV44_RS00635 112585..113880 - 1296 WP_000752772 type 4b pilus protein PilO2 -
HTV44_RS00640 113873..115555 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTV44_RS00645 115569..116006 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTV44_RS00650 116006..117073 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3728 GenBank   NZ_MK181559
Plasmid name   p199 Incompatibility group   IncI1
Plasmid size   122616 bp Coordinate of oriT [Strand]   63151..63239 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   aadA5, sul2, blaCTX-M-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -