Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103284
Name   oriT_pESBL20140131 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK181565 (52936..53024 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pESBL20140131
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2453 GenBank   WP_025492051
Name   nikB_HTW86_RS00370_pESBL20140131 insolico UniProt ID   A0A075UR71
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104022.47 Da        Isoelectric Point: 7.4265

>WP_025492051.1 IncI1-type relaxase NikB [Escherichia coli]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYAIRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A075UR71


T4CP

ID   2227 GenBank   WP_001289276
Name   trbC_HTW86_RS00375_pESBL20140131 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 58397..97863

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTW86_RS00375 56113..58404 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTW86_RS00380 58397..59467 - 1071 WP_000151582 IncI1-type conjugal transfer protein TrbB trbB
HTW86_RS00385 59486..60694 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTW86_RS00390 61001..61780 - 780 WP_275450201 protein FinQ -
HTW86_RS00395 62407..62559 + 153 WP_001303307 Hok/Gef family protein -
HTW86_RS00400 62631..62882 - 252 WP_001291965 hypothetical protein -
HTW86_RS00405 63805..64089 - 285 WP_014640317 hypothetical protein -
HTW86_RS00410 64190..64399 - 210 Protein_78 hemolysin expression modulator Hha -
HTW86_RS00415 64497..65111 - 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
HTW86_RS00420 65187..67355 - 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
HTW86_RS00425 67452..68036 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTW86_RS00430 68065..69267 - 1203 WP_001189159 IncI1-type conjugal transfer protein TraW traW
HTW86_RS00435 69234..69848 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
HTW86_RS00440 69848..72892 - 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
HTW86_RS00445 72982..73782 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTW86_RS00450 73766..73954 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTW86_RS00455 74018..74422 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
HTW86_RS00460 74473..75000 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTW86_RS00465 75000..75704 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTW86_RS00470 75704..76993 - 1290 WP_001271997 conjugal transfer protein TraO traO
HTW86_RS00475 76996..77979 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
HTW86_RS00480 77990..78682 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
HTW86_RS00485 78679..79026 - 348 WP_001055900 conjugal transfer protein traL
HTW86_RS00490 79044..82811 - 3768 WP_001141539 LPD7 domain-containing protein -
HTW86_RS00495 82901..83452 - 552 WP_000014584 phospholipase D family protein -
HTW86_RS00500 83467..83757 - 291 WP_001299214 hypothetical protein traK
HTW86_RS00505 83754..84902 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTW86_RS00510 84899..85717 - 819 WP_025492003 IncI1-type conjugal transfer lipoprotein TraI traI
HTW86_RS00515 85714..86172 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTW86_RS00520 86567..87151 - 585 WP_000977522 histidine phosphatase family protein -
HTW86_RS00525 87211..88413 - 1203 WP_000976349 conjugal transfer protein TraF -
HTW86_RS00530 88499..89323 - 825 WP_001238927 conjugal transfer protein TraE traE
HTW86_RS00535 89474..90628 - 1155 WP_001139958 site-specific integrase -
HTW86_RS00640 91712..91936 + 225 Protein_104 hypothetical protein -
HTW86_RS00545 91933..93357 - 1425 WP_024199048 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTW86_RS00550 93357..94013 - 657 WP_001389386 prepilin peptidase -
HTW86_RS00555 93998..94558 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTW86_RS00560 94568..95182 - 615 WP_000959786 type 4 pilus major pilin -
HTW86_RS00565 95200..96297 - 1098 WP_001208805 type II secretion system F family protein -
HTW86_RS00570 96310..97863 - 1554 WP_025492055 ATPase, T2SS/T4P/T4SS family virB11
HTW86_RS00575 97874..98326 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
HTW86_RS00580 98313..99608 - 1296 WP_000752772 type 4b pilus protein PilO2 -
HTW86_RS00585 99601..101283 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTW86_RS00590 101297..101734 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTW86_RS00595 101734..102801 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3727 GenBank   NZ_MK181565
Plasmid name   pESBL20140131 Incompatibility group   IncI1
Plasmid size   108344 bp Coordinate of oriT [Strand]   52936..53024 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   sul2, blaCTX-M-1, sul1, qacE, ant(3'')-Ia, dfrA1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2