Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103283
Name   oriT_p14011252 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK181561 (51289..51377 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p14011252
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2452 GenBank   WP_024143648
Name   nikB_HTV54_RS00335_p14011252 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   1051 GenBank   WP_001283947
Name   WP_001283947_p14011252 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2226 GenBank   WP_001289276
Name   trbC_HTV54_RS00340_p14011252 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56755..97733

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTV54_RS00340 54471..56762 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTV54_RS00345 56755..57825 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTV54_RS00350 57844..59052 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTV54_RS00355 59344..59496 + 153 WP_001331364 Hok/Gef family protein -
HTV54_RS00360 59568..59819 - 252 WP_001291964 hypothetical protein -
HTV54_RS00635 60318..60413 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HTV54_RS00615 60478..60654 - 177 WP_001054904 hypothetical protein -
HTV54_RS00370 61046..61255 + 210 WP_000062603 HEAT repeat domain-containing protein -
HTV54_RS00375 61327..62001 - 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTV54_RS00380 62051..64219 - 2169 WP_021537350 DotA/TraY family protein traY
HTV54_RS00385 64316..64900 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTV54_RS00390 64929..66131 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HTV54_RS00395 66098..66712 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HTV54_RS00400 66712..69756 - 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
HTV54_RS00405 69846..70646 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTV54_RS00410 70630..70818 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTV54_RS00415 70882..71286 - 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
HTV54_RS00420 71337..71864 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTV54_RS00425 71864..72568 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTV54_RS00430 72568..73857 - 1290 WP_001272003 conjugal transfer protein TraO traO
HTV54_RS00435 73860..74843 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HTV54_RS00440 74854..75546 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTV54_RS00445 75543..75890 - 348 WP_001055900 conjugal transfer protein traL
HTV54_RS00450 75908..79675 - 3768 WP_001141527 LPD7 domain-containing protein -
HTV54_RS00455 79764..80315 - 552 WP_000014583 phospholipase D family protein -
HTV54_RS00460 80330..80620 - 291 WP_001299214 hypothetical protein traK
HTV54_RS00465 80617..81765 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTV54_RS00470 81762..82580 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HTV54_RS00475 82577..83035 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTV54_RS00480 83430..84014 - 585 WP_000977522 histidine phosphatase family protein -
HTV54_RS00485 84074..85276 - 1203 WP_000976351 conjugal transfer protein TraF -
HTV54_RS00490 85361..86185 - 825 WP_001238939 conjugal transfer protein TraE traE
HTV54_RS00495 86336..87490 - 1155 WP_001139957 site-specific integrase -
HTV54_RS00500 87489..87806 + 318 WP_001558195 hypothetical protein -
HTV54_RS00630 88309..88518 - 210 Protein_100 hypothetical protein -
HTV54_RS00505 88518..88862 + 345 Protein_101 WbuC family cupin fold metalloprotein -
HTV54_RS00510 88909..89784 - 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
HTV54_RS00515 90072..91334 - 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HTV54_RS00650 91564..91806 + 243 Protein_104 prepilin -
HTV54_RS00525 91803..93227 - 1425 WP_001389385 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTV54_RS00530 93227..93883 - 657 WP_001193553 prepilin peptidase -
HTV54_RS00535 93868..94428 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTV54_RS00540 94438..95052 - 615 WP_000959785 type 4 pilus major pilin -
HTV54_RS00545 95070..96167 - 1098 WP_001208805 type II secretion system F family protein -
HTV54_RS00550 96180..97733 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HTV54_RS00555 97744..98196 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
HTV54_RS00560 98183..99478 - 1296 WP_000752772 type 4b pilus protein PilO2 -
HTV54_RS00565 99471..101153 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTV54_RS00570 101167..101604 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTV54_RS00575 101604..102671 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3726 GenBank   NZ_MK181561
Plasmid name   p14011252 Incompatibility group   IncI1
Plasmid size   108214 bp Coordinate of oriT [Strand]   51289..51377 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   dfrA17, aadA5, sul2, blaCTX-M-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -