Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103282
Name   oriT_p15090172 in_silico
Organism   Escherichia coli
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MK181562 (50780..50868 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_p15090172
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2451 GenBank   WP_024143648
Name   nikB_HTV66_RS00350_p15090172 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   1050 GenBank   WP_001283947
Name   WP_001283947_p15090172 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2225 GenBank   WP_001289276
Name   trbC_HTV66_RS00355_p15090172 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56246..97224

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTV66_RS00355 53962..56253 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
HTV66_RS00360 56246..57316 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HTV66_RS00365 57335..58543 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HTV66_RS00370 58835..58987 + 153 WP_001331364 Hok/Gef family protein -
HTV66_RS00375 59059..59310 - 252 WP_001291964 hypothetical protein -
HTV66_RS00655 59809..59904 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HTV66_RS00380 59969..60145 - 177 WP_001054904 hypothetical protein -
HTV66_RS00385 60537..60746 + 210 WP_000062603 HEAT repeat domain-containing protein -
HTV66_RS00390 60818..61492 - 675 WP_223866377 plasmid IncI1-type surface exclusion protein ExcA -
HTV66_RS00395 61542..63710 - 2169 WP_021537350 DotA/TraY family protein traY
HTV66_RS00400 63807..64391 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
HTV66_RS00405 64420..65622 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
HTV66_RS00410 65589..66203 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HTV66_RS00415 66203..69247 - 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
HTV66_RS00420 69337..70137 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
HTV66_RS00425 70121..70309 - 189 WP_001277255 putative conjugal transfer protein TraS -
HTV66_RS00430 70373..70777 - 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
HTV66_RS00435 70828..71355 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HTV66_RS00440 71355..72059 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
HTV66_RS00445 72059..73348 - 1290 WP_001272003 conjugal transfer protein TraO traO
HTV66_RS00450 73351..74334 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
HTV66_RS00455 74345..75037 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
HTV66_RS00460 75034..75381 - 348 WP_001055900 conjugal transfer protein traL
HTV66_RS00465 75399..79166 - 3768 WP_001141527 LPD7 domain-containing protein -
HTV66_RS00470 79255..79806 - 552 WP_000014583 phospholipase D family protein -
HTV66_RS00475 79821..80111 - 291 WP_001299214 hypothetical protein traK
HTV66_RS00480 80108..81256 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HTV66_RS00485 81253..82071 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
HTV66_RS00490 82068..82526 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HTV66_RS00495 82921..83505 - 585 WP_000977522 histidine phosphatase family protein -
HTV66_RS00500 83565..84767 - 1203 WP_000976351 conjugal transfer protein TraF -
HTV66_RS00505 84852..85676 - 825 WP_001238939 conjugal transfer protein TraE traE
HTV66_RS00510 85827..86981 - 1155 WP_001139957 site-specific integrase -
HTV66_RS00645 86965..87387 + 423 WP_162847923 prepilin, shufflon protein A -
HTV66_RS00670 87384..87614 - 231 Protein_101 prepilin -
HTV66_RS00525 88357..89619 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
HTV66_RS00530 89907..90782 + 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
HTV66_RS00535 90829..91173 - 345 Protein_104 WbuC family cupin fold metalloprotein -
HTV66_RS00650 91173..91382 + 210 Protein_105 hypothetical protein -
HTV66_RS00540 91384..92718 - 1335 WP_024132209 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HTV66_RS00545 92718..93374 - 657 WP_001193553 prepilin peptidase -
HTV66_RS00550 93359..93919 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
HTV66_RS00555 93929..94543 - 615 WP_000959785 type 4 pilus major pilin -
HTV66_RS00560 94561..95658 - 1098 WP_001208805 type II secretion system F family protein -
HTV66_RS00565 95671..97224 - 1554 WP_000362202 ATPase, T2SS/T4P/T4SS family virB11
HTV66_RS00570 97235..97687 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
HTV66_RS00575 97674..98969 - 1296 WP_000752772 type 4b pilus protein PilO2 -
HTV66_RS00580 98962..100644 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HTV66_RS00585 100658..101095 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
HTV66_RS00590 101095..102162 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3725 GenBank   NZ_MK181562
Plasmid name   p15090172 Incompatibility group   IncI1
Plasmid size   107705 bp Coordinate of oriT [Strand]   50780..50868 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   aadA5, sul2, blaCTX-M-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -