Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103280 |
| Name | oriT_p14006165  |
| Organism | Escherichia coli |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MK181560 (51736..51824 [-], 89 nt) |
| oriT length | 89 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 89 nt
>oriT_p14006165
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2449 | GenBank | WP_024143648 |
| Name | nikB_HTV35_RS00340_p14006165 |
UniProt ID | A0A023JAU0 |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 104010.41 Da Isoelectric Point: 7.4265
>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A023JAU0 |
Auxiliary protein
| ID | 1048 | GenBank | WP_001283947 |
| Name | WP_001283947_p14006165 |
UniProt ID | A0A142CMC2 |
| Length | 110 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12613.57 Da Isoelectric Point: 10.7463
>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142CMC2 |
T4CP
| ID | 2224 | GenBank | WP_001289276 |
| Name | trbC_HTV35_RS00345_p14006165 |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86941.11 Da Isoelectric Point: 6.7713
>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 57202..99493
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTV35_RS00345 | 54918..57209 | - | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
| HTV35_RS00350 | 57202..58272 | - | 1071 | WP_000151583 | IncI1-type conjugal transfer protein TrbB | trbB |
| HTV35_RS00355 | 58291..59499 | - | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| HTV35_RS00360 | 59791..59943 | + | 153 | WP_001331364 | Hok/Gef family protein | - |
| HTV35_RS00365 | 60015..60266 | - | 252 | WP_001291964 | hypothetical protein | - |
| HTV35_RS00665 | 60765..60860 | + | 96 | WP_001303310 | DinQ-like type I toxin DqlB | - |
| HTV35_RS00370 | 60925..61209 | - | 285 | WP_031942457 | hypothetical protein | - |
| HTV35_RS00375 | 61493..61702 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| HTV35_RS00380 | 61774..62448 | - | 675 | WP_223866377 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HTV35_RS00385 | 62498..64495 | - | 1998 | Protein_75 | DotA/TraY family protein | - |
| HTV35_RS00390 | 64503..65716 | + | 1214 | WP_162858271 | IS3 family transposase | - |
| HTV35_RS00395 | 65758..65979 | - | 222 | Protein_77 | hypothetical protein | - |
| HTV35_RS00400 | 66076..66660 | - | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| HTV35_RS00405 | 66689..67891 | - | 1203 | WP_001189160 | IncI1-type conjugal transfer protein TraW | traW |
| HTV35_RS00410 | 67858..68472 | - | 615 | WP_000337398 | IncI1-type conjugal transfer protein TraV | traV |
| HTV35_RS00415 | 68472..71516 | - | 3045 | WP_021265678 | IncI1-type conjugal transfer protein TraU | traU |
| HTV35_RS00420 | 71606..72406 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| HTV35_RS00425 | 72390..72578 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| HTV35_RS00430 | 72642..73046 | - | 405 | WP_000086958 | IncI1-type conjugal transfer protein TraR | traR |
| HTV35_RS00435 | 73097..73624 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| HTV35_RS00440 | 73624..74328 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| HTV35_RS00445 | 74328..75617 | - | 1290 | WP_001272003 | conjugal transfer protein TraO | traO |
| HTV35_RS00450 | 75620..76603 | - | 984 | WP_001191877 | IncI1-type conjugal transfer protein TraN | traN |
| HTV35_RS00455 | 76614..77306 | - | 693 | WP_000138550 | DotI/IcmL family type IV secretion protein | traM |
| HTV35_RS00460 | 77303..77650 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| HTV35_RS00465 | 77668..81435 | - | 3768 | WP_001141527 | LPD7 domain-containing protein | - |
| HTV35_RS00470 | 81524..82075 | - | 552 | WP_000014583 | phospholipase D family protein | - |
| HTV35_RS00650 | 82090..82380 | - | 291 | WP_001299214 | hypothetical protein | traK |
| HTV35_RS00480 | 82377..83525 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| HTV35_RS00485 | 83522..84340 | - | 819 | WP_000646098 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| HTV35_RS00490 | 84337..84795 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| HTV35_RS00495 | 85190..85774 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| HTV35_RS00500 | 85834..87036 | - | 1203 | WP_000976351 | conjugal transfer protein TraF | - |
| HTV35_RS00505 | 87121..87945 | - | 825 | WP_001238939 | conjugal transfer protein TraE | traE |
| HTV35_RS00510 | 88096..89250 | - | 1155 | WP_001139957 | site-specific integrase | - |
| HTV35_RS00655 | 89234..89656 | + | 423 | WP_162847923 | prepilin, shufflon protein A | - |
| HTV35_RS00680 | 89653..89874 | - | 222 | Protein_102 | prepilin | - |
| HTV35_RS00660 | 90650..90859 | - | 210 | Protein_103 | hypothetical protein | - |
| HTV35_RS00520 | 90859..91203 | + | 345 | Protein_104 | WbuC family cupin fold metalloprotein | - |
| HTV35_RS00525 | 91250..92125 | - | 876 | WP_013188473 | extended-spectrum class A beta-lactamase CTX-M-1 | - |
| HTV35_RS00530 | 92413..93675 | - | 1263 | WP_000608644 | IS1380-like element ISEcp1 family transposase | - |
| HTV35_RS00535 | 93893..94987 | - | 1095 | Protein_107 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HTV35_RS00540 | 94987..95643 | - | 657 | WP_001193553 | prepilin peptidase | - |
| HTV35_RS00545 | 95628..96188 | - | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| HTV35_RS00550 | 96198..96812 | - | 615 | WP_000959785 | type 4 pilus major pilin | - |
| HTV35_RS00555 | 96830..97927 | - | 1098 | WP_001208805 | type II secretion system F family protein | - |
| HTV35_RS00560 | 97940..99493 | - | 1554 | WP_000362202 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HTV35_RS00565 | 99504..99956 | - | 453 | WP_001247334 | type IV pilus biogenesis protein PilP | - |
| HTV35_RS00570 | 99943..100110 | - | 168 | Protein_114 | type 4b pilus protein PilO2 | - |
| HTV35_RS00580 | 101421..102551 | - | 1131 | Protein_116 | type 4b pilus protein PilO2 | - |
| HTV35_RS00585 | 102544..104226 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
Host bacterium
| ID | 3723 | GenBank | NZ_MK181560 |
| Plasmid name | p14006165 | Incompatibility group | IncI1 |
| Plasmid size | 111287 bp | Coordinate of oriT [Strand] | 51736..51824 [-] |
| Host baterium | Escherichia coli |
Cargo genes
| Drug resistance gene | dfrA17, aadA5, sul2, blaCTX-M-1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |