Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103270
Name   oriT_pESBL176 in_silico
Organism   Escherichia coli strain E. coli UB-ESBL176
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT230168 (19577..19677 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pESBL176
TATTTATTTTTTTATTTTTTAAATCAGTGTGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2443 GenBank   WP_011407157
Name   TrwC_G6845_RS00115_pESBL176 insolico UniProt ID   A0A740S2B6
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120179.41 Da        Isoelectric Point: 6.5766

>WP_011407157.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLSGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB A0A740S2B6


Auxiliary protein

ID   1042 GenBank   WP_001532083
Name   WP_001532083_pESBL176 insolico UniProt ID   A8R734
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001532083.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCLEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A8R734


T4CP

ID   2213 GenBank   WP_000342688
Name   t4cp2_G6845_RS00120_pESBL176 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2587..19010

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6845_RS00005 1..195 + 195 WP_000321898 DDE-type integrase/transposase/recombinase -
G6845_RS00010 257..454 - 198 WP_000844102 hypothetical protein -
G6845_RS00015 (G6845_00001) 459..1100 - 642 WP_001694850 restriction endonuclease -
G6845_RS00020 (G6845_00002) 1156..1389 - 234 WP_001191790 hypothetical protein -
G6845_RS00025 (G6845_00003) 1503..1817 - 315 WP_000734776 TrbM/KikA/MpfK family conjugal transfer protein -
G6845_RS00030 (G6845_00004) 1780..2157 - 378 WP_181727003 hypothetical protein -
G6845_RS00035 (G6845_00005) 2173..2544 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
G6845_RS00040 (G6845_00006) 2587..3321 + 735 WP_000033783 lytic transglycosylase domain-containing protein virB1
G6845_RS00045 (G6845_00007) 3330..3611 + 282 WP_000440698 transcriptional repressor KorA -
G6845_RS00050 (G6845_00008) 3621..3914 + 294 WP_000209137 TrbC/VirB2 family protein virB2
G6845_RS00055 (G6845_00009) 3964..4281 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
G6845_RS00060 (G6845_00010) 4281..6881 + 2601 WP_001200711 VirB4 family type IV secretion/conjugal transfer ATPase virb4
G6845_RS00065 (G6845_00011) 6899..7612 + 714 WP_000749362 type IV secretion system protein virB5
G6845_RS00070 (G6845_00012) 7620..7847 + 228 WP_000734973 IncN-type entry exclusion lipoprotein EexN -
G6845_RS00075 (G6845_00013) 7863..8903 + 1041 WP_000886022 type IV secretion system protein virB6
G6845_RS00080 8974..9132 + 159 WP_012561180 hypothetical protein -
G6845_RS00085 (G6845_00014) 9122..9820 + 699 WP_000646594 type IV secretion system protein virB8
G6845_RS00090 (G6845_00015) 9831..10715 + 885 WP_000735067 TrbG/VirB9 family P-type conjugative transfer protein virB9
G6845_RS00095 (G6845_00016) 10715..11875 + 1161 WP_000101711 type IV secretion system protein VirB10 virB10
G6845_RS00100 (G6845_00017) 11917..12912 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
G6845_RS00105 (G6845_00018) 12912..13445 + 534 WP_000731968 phospholipase D family protein -
G6845_RS00110 (G6845_00019) 13619..14245 - 627 WP_000434930 DUF6710 family protein -
G6845_RS00115 (G6845_00020) 14245..17481 - 3237 WP_011407157 MobF family relaxase -
G6845_RS00120 (G6845_00021) 17481..19010 - 1530 WP_000342688 type IV secretion system DNA-binding domain-containing protein virb4
G6845_RS00125 (G6845_00022) 19012..19428 - 417 WP_001532083 hypothetical protein -
G6845_RS00160 (G6845_00023) 19941..20360 + 420 WP_000802909 plasmid stabilization protein StbA -
G6845_RS00135 (G6845_00024) 20369..21085 + 717 WP_000861577 StbB family protein -
G6845_RS00140 (G6845_00025) 21087..21455 + 369 WP_000414913 plasmid stabilization protein StbC -
G6845_RS00145 (G6845_00026) 21637..21981 + 345 WP_223870441 hypothetical protein -
G6845_RS00150 (G6845_00027) 22092..22412 - 321 WP_000211833 protein CcgAII -
G6845_RS00155 (G6845_00028) 22467..22646 - 180 WP_000214483 protein CcgAI -
G6845_RS00165 22755..22943 - 189 WP_223870442 hypothetical protein -

Host bacterium

ID   3713 GenBank   NZ_MT230168
Plasmid name   pESBL176 Incompatibility group   -
Plasmid size   22943 bp Coordinate of oriT [Strand]   19577..19677 [+]
Host baterium   Escherichia coli strain E. coli UB-ESBL176

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -