Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103267
Name   oriT_pEC0674 in_silico
Organism   Escherichia coli strain PEC15-AB00674-RC
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MF684783 (5006..5039 [+], 34 nt)
oriT length   34 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 34 nt

>oriT_pEC0674
ACGATTTTGCGAAGCAAAGTCTAGTGAGTACACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2441 GenBank   WP_000024818
Name   Mob_Pre_HTO27_RS00030_pEC0674 insolico UniProt ID   A0A5W5SJI7
Length   322 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 322 a.a.        Molecular weight: 36743.68 Da        Isoelectric Point: 10.3759

>WP_000024818.1 MULTISPECIES: MobV family relaxase [Gammaproteobacteria]
MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCSVSKSTDQAMGRVRELLPEKRRKDAV
LAVEYVMTASPEWWKEATPRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLS
AKEFIGGRSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRK
GIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQ
MTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-185)


  Protein structure


Source ID Structure
AlphaFold DB A0A5W5SJI7


Host bacterium

ID   3710 GenBank   NZ_MF684783
Plasmid name   pEC0674 Incompatibility group   -
Plasmid size   6268 bp Coordinate of oriT [Strand]   5006..5039 [+]
Host baterium   Escherichia coli strain PEC15-AB00674-RC

Cargo genes

Drug resistance gene   mcr-5.1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -