Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103262 |
| Name | oriT_pMCR3_WCHEC-LL123  |
| Organism | Escherichia coli strain WCHEC-LL123 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MF489760 (18862..18964 [+], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | 64..70, 73..79 (CCACCGT..ACGGTGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 103 nt
>oriT_pMCR3_WCHEC-LL123
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2438 | GenBank | WP_077270386 |
| Name | Relaxase_CGG75_RS00105_pMCR3_WCHEC-LL123 |
UniProt ID | _ |
| Length | 869 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 869 a.a. Molecular weight: 96535.47 Da Isoelectric Point: 10.4435
>WP_077270386.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKVLGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVKAECAEQLLNANSWQELHQAMRQNGLELRQK
GNGFVIAGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAANVKRPGDRPP
PMRENRQVHLGDVPVMQINSGKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRSGRLKRATIKLLKGPGVNKRYLYGLASKVLGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGKRLDVSR
DAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKKAAREREYI
HIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDAGRERSGNG
NATGANEPGVTGRSTSADAGNGYGTSSSRRRTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKP
NVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAE
VKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2205 | GenBank | WP_032310210 |
| Name | t4cp2_CGG75_RS00095_pMCR3_WCHEC-LL123 |
UniProt ID | _ |
| Length | 648 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 648 a.a. Molecular weight: 71711.82 Da Isoelectric Point: 8.3842
>WP_032310210.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAVAANDDASNEAIAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35806..46055
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CGG75_RS00215 (BCLKEOBJ_00049) | 30807..32006 | + | 1200 | WP_000948429 | IS91 family transposase | - |
| CGG75_RS00220 (BCLKEOBJ_00050) | 32250..32558 | - | 309 | WP_003019666 | HigA family addiction module antitoxin | - |
| CGG75_RS00225 (BCLKEOBJ_00051) | 32567..32866 | - | 300 | WP_071443968 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| CGG75_RS00230 (BCLKEOBJ_00052) | 33880..34758 | - | 879 | WP_048207660 | plasmid replication initiator TrfA | - |
| CGG75_RS00240 (BCLKEOBJ_00054) | 35148..35552 | + | 405 | WP_048207662 | transcriptional regulator | - |
| CGG75_RS00245 (BCLKEOBJ_00055) | 35806..36693 | + | 888 | WP_235927881 | P-type conjugative transfer ATPase TrbB | virB11 |
| CGG75_RS00250 (BCLKEOBJ_00056) | 36807..37190 | + | 384 | WP_003019650 | TrbC/VirB2 family protein | virB2 |
| CGG75_RS00255 (BCLKEOBJ_00057) | 37193..37519 | + | 327 | WP_003019648 | conjugal transfer protein TrbD | virB3 |
| CGG75_RS00260 (BCLKEOBJ_00058) | 37516..40059 | + | 2544 | WP_071443970 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| CGG75_RS00265 (BCLKEOBJ_00059) | 40056..40763 | + | 708 | WP_048207665 | VirB8/TrbF family protein | virB8 |
| CGG75_RS00270 (BCLKEOBJ_00060) | 40779..41669 | + | 891 | WP_003019641 | P-type conjugative transfer protein TrbG | virB9 |
| CGG75_RS00275 (BCLKEOBJ_00061) | 41672..42124 | + | 453 | WP_032194780 | conjugal transfer protein TrbH | - |
| CGG75_RS00280 (BCLKEOBJ_00062) | 42128..43522 | + | 1395 | WP_048207667 | TrbI/VirB10 family protein | virB10 |
| CGG75_RS00285 (BCLKEOBJ_00063) | 43535..44305 | + | 771 | WP_046960647 | P-type conjugative transfer protein TrbJ | virB5 |
| CGG75_RS00290 | 44318..44491 | + | 174 | WP_024252956 | entry exclusion lipoprotein TrbK | - |
| CGG75_RS00295 (BCLKEOBJ_00064) | 44496..46055 | + | 1560 | WP_048207669 | P-type conjugative transfer protein TrbL | virB6 |
| CGG75_RS00300 (BCLKEOBJ_00065) | 46066..46371 | + | 306 | WP_032310194 | lipoprotein | - |
| CGG75_RS00305 (BCLKEOBJ_00066) | 46387..47157 | + | 771 | WP_048207671 | conjugal transfer protein | - |
| CGG75_RS00310 (BCLKEOBJ_00067) | 47174..47860 | + | 687 | WP_053065212 | TraX family protein | - |
| CGG75_RS00315 (BCLKEOBJ_00068) | 48057..48911 | - | 855 | Protein_64 | Tn3 family transposase | - |
| CGG75_RS00320 (BCLKEOBJ_00069) | 49180..50805 | + | 1626 | WP_089613755 | phosphoethanolamine--lipid A transferase MCR-3.5 | - |
Host bacterium
| ID | 3705 | GenBank | NZ_MF489760 |
| Plasmid name | pMCR3_WCHEC-LL123 | Incompatibility group | IncP1 |
| Plasmid size | 52208 bp | Coordinate of oriT [Strand] | 18862..18964 [+] |
| Host baterium | Escherichia coli strain WCHEC-LL123 |
Cargo genes
| Drug resistance gene | mcr-3.5 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |