Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103259 |
| Name | oriT_pPs7B44  |
| Organism | Pseudomonas syringae pv. syringae strain 7B44 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY362373 (65447..65627 [+], 181 nt) |
| oriT length | 181 nt |
| IRs (inverted repeats) | 80..87, 91..98 (CAAAGGGG..CCCCTTTG) 42..47, 56..61 (AAAAAG..CTTTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 181 nt
>oriT_pPs7B44
ATCTCGAAATCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTAGCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCAGAGCGGGAAAGCGTTGTTGTTGTCGTTGTTGGTTGTCGTCATTCCAGGTGCCCCCAGGAGGTGATCTTGTGGC
ATCTCGAAATCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTAGCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCAGAGCGGGAAAGCGTTGTTGTTGTCGTTGTTGGTTGTCGTCATTCCAGGTGCCCCCAGGAGGTGATCTTGTGGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2436 | GenBank | WP_172689534 |
| Name | Relaxase_HTL61_RS00390_pPs7B44 |
UniProt ID | _ |
| Length | 1252 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1252 a.a. Molecular weight: 138526.22 Da Isoelectric Point: 9.4839
>WP_172689534.1 LPD7 domain-containing protein [Pseudomonas syringae]
MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYLTFTLSFKEDT
VSPELLKAVTTDFKNFFMHAYKPEEFNMYAEAHLPKMKTVTDRKTGEVIERKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQQLVKQVIER
GVTSRADFYALVAEHGETRIRNEGKDNEYISVKLPGDAKGTNLKDTIFQDAFIVRRELKKPPLEASVIQE
RLLAWPQRAREIKYVNKATPRFRKQYSQASPEERVRLLAERETNFYRAHGDDYESVHTGQRQRDHQRSPA
ETTGRRIAASADGVQDLSVSHVADHRQAGPTRSRDGAVLLPSDAHVHVGQSQPGGDPGLRPPVPAGGRGR
RNGSTAERGRGGSGPAAVSQEATGTATPAGATGRRRAGAGKPRNARVRAGRVVPPYAKNPHRVANIADIE
ERGRRLFDPLKKPSDNALVFYRSPSVPAVPKAVSATTTPGRSTGGHRPRTSGKPRPPRQWRPGAVVPPYA
KNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNKHASTVAAYFTRQAQHNQIAPAHRRA
IRRIDQQYFALRRAVFSDHRLTRQDKAQLVSVLTFERLKAREQFHNPKPNIEVNLMGSAAIRNLLDDEKE
DPGFSISGARGPGPEGVRERVKRVMDRFAKQVDPVAASERARDLSAKDLYTRKAKFSQNVHYLDKQTDKT
LFVDTGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMNQSL
ADRRAELDIERDGQSIGPATDLPRHVDDATRDVRDQADRLGVTVPIEALYGQGKTADQVSQALATQLDTV
PEPERIAFVEKVAISLGIPERGQPKGDQAFAQWQAQRAHPDVDSTAPATSEAQTSTPQPVSTSAGTSPAP
APVNPEAAKPTPANDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGKDHAIWLIAADDKTPEA
AAMLTAYMENDSYREAFKASVVDLYKQVENSPKLIDDLDQLTAMAAQIVNEVEERLFPTPQAATGQTAPS
RSKVIEGTLIEHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDADVKQGDQVRLQDLGTQP
VVVQVIEEDGTITDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEDDGMSVD
MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYLTFTLSFKEDT
VSPELLKAVTTDFKNFFMHAYKPEEFNMYAEAHLPKMKTVTDRKTGEVIERKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQQLVKQVIER
GVTSRADFYALVAEHGETRIRNEGKDNEYISVKLPGDAKGTNLKDTIFQDAFIVRRELKKPPLEASVIQE
RLLAWPQRAREIKYVNKATPRFRKQYSQASPEERVRLLAERETNFYRAHGDDYESVHTGQRQRDHQRSPA
ETTGRRIAASADGVQDLSVSHVADHRQAGPTRSRDGAVLLPSDAHVHVGQSQPGGDPGLRPPVPAGGRGR
RNGSTAERGRGGSGPAAVSQEATGTATPAGATGRRRAGAGKPRNARVRAGRVVPPYAKNPHRVANIADIE
ERGRRLFDPLKKPSDNALVFYRSPSVPAVPKAVSATTTPGRSTGGHRPRTSGKPRPPRQWRPGAVVPPYA
KNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNKHASTVAAYFTRQAQHNQIAPAHRRA
IRRIDQQYFALRRAVFSDHRLTRQDKAQLVSVLTFERLKAREQFHNPKPNIEVNLMGSAAIRNLLDDEKE
DPGFSISGARGPGPEGVRERVKRVMDRFAKQVDPVAASERARDLSAKDLYTRKAKFSQNVHYLDKQTDKT
LFVDTGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMNQSL
ADRRAELDIERDGQSIGPATDLPRHVDDATRDVRDQADRLGVTVPIEALYGQGKTADQVSQALATQLDTV
PEPERIAFVEKVAISLGIPERGQPKGDQAFAQWQAQRAHPDVDSTAPATSEAQTSTPQPVSTSAGTSPAP
APVNPEAAKPTPANDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGKDHAIWLIAADDKTPEA
AAMLTAYMENDSYREAFKASVVDLYKQVENSPKLIDDLDQLTAMAAQIVNEVEERLFPTPQAATGQTAPS
RSKVIEGTLIEHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDADVKQGDQVRLQDLGTQP
VVVQVIEEDGTITDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEDDGMSVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2202 | GenBank | WP_172689528 |
| Name | t4cp2_HTL61_RS00345_pPs7B44 |
UniProt ID | _ |
| Length | 550 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 550 a.a. Molecular weight: 61848.40 Da Isoelectric Point: 5.8059
>WP_172689528.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Pseudomonas]
MARGKSKPISPDNPQERIEEHPAFLLGKHPTEDSFLASYGQQFVMLAAPPGSMKGVSAVIPNLLSYPDSM
VVNDPKFENWEITSGFRASAGQKVYRFSPERLETHRWNPNSAINRDPLYRLGDIRTLARVLFVSDNPKNQ
EWYNKAGNVFSSILLYLMETPEMPFTLPQAYEIGSLGTGMGTWAQQIIELRSIGPNALSFETLRELNGVY
EASKNKSSGWSTTVDIVRDVLSVYAEKTVAWAVSGDDIDFAKMREEKTTVYFSVTEGNLKKYGPLMNLFF
TQAIRLNAKVLPEQGGHCADGTLRYKYQLALMMDEFAIMGRMEIMETAPALTRGAGLRFFLIFQGKDQIR
AVYGEEAANGIMKAIHNEIVFAPGDIKLAEEYSRRLGNTTVRVHNQSLNRQRHEVGARGQTDSYSEQPRP
LMFPQEVNELPFDKQLIFVQGNRQTEPMKILARKIIYFEEEVFKARQKMTPPPLPVGDASKIDALTVPVR
TIESKVAVADTKPMQAEQRQRWNPKDKASEVAQAEADKAQPVEVEPDPEPVQAEDTSEPM
MARGKSKPISPDNPQERIEEHPAFLLGKHPTEDSFLASYGQQFVMLAAPPGSMKGVSAVIPNLLSYPDSM
VVNDPKFENWEITSGFRASAGQKVYRFSPERLETHRWNPNSAINRDPLYRLGDIRTLARVLFVSDNPKNQ
EWYNKAGNVFSSILLYLMETPEMPFTLPQAYEIGSLGTGMGTWAQQIIELRSIGPNALSFETLRELNGVY
EASKNKSSGWSTTVDIVRDVLSVYAEKTVAWAVSGDDIDFAKMREEKTTVYFSVTEGNLKKYGPLMNLFF
TQAIRLNAKVLPEQGGHCADGTLRYKYQLALMMDEFAIMGRMEIMETAPALTRGAGLRFFLIFQGKDQIR
AVYGEEAANGIMKAIHNEIVFAPGDIKLAEEYSRRLGNTTVRVHNQSLNRQRHEVGARGQTDSYSEQPRP
LMFPQEVNELPFDKQLIFVQGNRQTEPMKILARKIIYFEEEVFKARQKMTPPPLPVGDASKIDALTVPVR
TIESKVAVADTKPMQAEQRQRWNPKDKASEVAQAEADKAQPVEVEPDPEPVQAEDTSEPM
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 44456..54831
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTL61_RS00235 | 39786..40112 | + | 327 | WP_025391071 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTL61_RS00240 | 40224..40511 | + | 288 | WP_025391072 | XRE family transcriptional regulator | - |
| HTL61_RS00245 | 40701..41474 | - | 774 | WP_017279416 | helix-turn-helix transcriptional regulator | - |
| HTL61_RS00250 | 41583..42683 | + | 1101 | WP_172689521 | polyamine ABC transporter substrate-binding protein | - |
| HTL61_RS00255 | 42780..42977 | + | 198 | WP_025391074 | hypothetical protein | - |
| HTL61_RS00260 | 42989..43384 | - | 396 | WP_046235773 | helix-turn-helix transcriptional regulator | - |
| HTL61_RS00265 | 43600..44112 | + | 513 | WP_055005870 | transcription termination/antitermination NusG family protein | - |
| HTL61_RS00270 | 44201..44443 | + | 243 | WP_172689522 | hypothetical protein | - |
| HTL61_RS00275 | 44456..45163 | + | 708 | WP_172689523 | lytic transglycosylase domain-containing protein | virB1 |
| HTL61_RS00280 | 45163..45498 | + | 336 | WP_025391077 | hypothetical protein | - |
| HTL61_RS00285 | 45511..45987 | + | 477 | WP_053486256 | VirB3 family type IV secretion system protein | virB3 |
| HTL61_RS00290 | 45926..48400 | + | 2475 | WP_237183646 | conjugal transfer protein | virb4 |
| HTL61_RS00295 | 48397..49083 | + | 687 | WP_025391079 | P-type DNA transfer protein VirB5 | - |
| HTL61_RS00300 | 49112..49465 | + | 354 | WP_122257564 | hypothetical protein | - |
| HTL61_RS00305 | 49476..50423 | + | 948 | WP_057445126 | type IV secretion system protein | virB6 |
| HTL61_RS00310 | 50846..51634 | + | 789 | WP_172689525 | type IV secretion system protein | virB8 |
| HTL61_RS00315 | 51624..52433 | + | 810 | WP_025391083 | P-type conjugative transfer protein VirB9 | virB9 |
| HTL61_RS00320 | 52420..53790 | + | 1371 | WP_025391084 | TrbI/VirB10 family protein | virB10 |
| HTL61_RS00325 | 53800..54831 | + | 1032 | WP_172689526 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTL61_RS00330 | 54841..55197 | + | 357 | WP_172689527 | hypothetical protein | - |
| HTL61_RS00335 | 55323..57713 | + | 2391 | WP_172689536 | ATP-binding protein | - |
| HTL61_RS00340 | 57800..58030 | + | 231 | WP_025391087 | hypothetical protein | - |
| HTL61_RS00345 | 58030..59682 | + | 1653 | WP_172689528 | type IV secretory system conjugative DNA transfer family protein | - |
Host bacterium
| ID | 3702 | GenBank | NZ_KY362373 |
| Plasmid name | pPs7B44 | Incompatibility group | - |
| Plasmid size | 71783 bp | Coordinate of oriT [Strand] | 65447..65627 [+] |
| Host baterium | Pseudomonas syringae pv. syringae strain 7B44 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsB, arsH, copS, copR, copC, copA |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |