Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103256 |
| Name | oriT_pPs0170  |
| Organism | Pseudomonas syringae pv. syringae strain UMAF0170 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY362372 (58231..58410 [+], 180 nt) |
| oriT length | 180 nt |
| IRs (inverted repeats) | 80..87, 91..98 (CAAAGGGG..CCCCTTTG) 42..47, 56..61 (AAAAAG..CTTTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 180 nt
>oriT_pPs0170
ATCTCGAATTCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCAGAGCGGGAAAGCGTTGTCGTTGTCGTTGTTGGTTGTTGTCATCCAGGTGCCCTCAGGAGGTGATTTTGTGGC
ATCTCGAATTCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCAGAGCGGGAAAGCGTTGTCGTTGTCGTTGTTGGTTGTTGTCATCCAGGTGCCCTCAGGAGGTGATTTTGTGGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2433 | GenBank | WP_172689661 |
| Name | Relaxase_HTL75_RS00345_pPs0170 |
UniProt ID | _ |
| Length | 1249 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1249 a.a. Molecular weight: 138590.83 Da Isoelectric Point: 8.9877
>WP_172689661.1 LPD7 domain-containing protein [Pseudomonas syringae]
MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYLTFTLSFKEDT
VSPELLKAVTTEFKNFFMHAYKPEEFNLYAEAHLPKMKTVTDRKTGEVIDRKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQQLVKQVIER
GVNSRADFYALVAEHGETRIRNEGKDNEYISVKLPGDAKGTNLKDTIFQDDFIVRRELRKPPLEASVIQE
RLLAWPQRAREIKYVNKATPKFRKQYSQASPEERVRLLAERETNFYRAHGDDYESVHTWQRQRDNQRSAA
ETTGRRTAAPADGLQDLSVSDVADHRQAGPTRSRDGALLLPSDAHVHVGQSQPGGDSGLRSSVPGGGRGR
RAGSTAERGRGGSQPAAVSQETKGTATPAGATGRRRAGAGKSRNTRVRAGRVVPPYAHNPHRVATIADIE
ERGRRLFDPLKRPSDNALVFYRSSSHPQVADQLPQARAATTTPVQSTAGRRPRSSGKPRPPRQWRPGAVP
PYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNKHASTVAAYFTRQAQHNQIAPAH
RRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVSVLTFERLKAREQFHKPQPNIEVNLMGSAAIRNLLDD
EKEDPGFSISGARGPGREGVRDQVKRIMDRFAKQVDPDAASERARDLSAKDLYTRKAKFSQNVHYLDKQT
DKTLFVDTGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMN
QSLADRRAELDIERDGQSIGPATDLPRHVDDATRDVRDQADRLGVTVPIEALYGQGKTADQVSQALTAQL
DTVPESERIAFVETVAITLGIPERGQPKGDQAFAQWQAQRAQPAANSAASATSEAQANVPTPSDAVPEPV
IPEAAKPALVNDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGEDHAIWIIATDDKTPEAAAL
LTAYMENDSYREAFKASIVAAYKQVENSPQSVDDLDHLTAMAAQIVNEVEERLFPSPQAATGQTAPSRSK
VIEGTLIDHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDADLKQGDQVRLRDLGTQPVVV
QVIEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEDDGMSVD
MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYLTFTLSFKEDT
VSPELLKAVTTEFKNFFMHAYKPEEFNLYAEAHLPKMKTVTDRKTGEVIDRKPHIHIIIPRINLLSGNEA
NPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDAASVLSRYKGDDFYGKNRQFKQQLVKQVIER
GVNSRADFYALVAEHGETRIRNEGKDNEYISVKLPGDAKGTNLKDTIFQDDFIVRRELRKPPLEASVIQE
RLLAWPQRAREIKYVNKATPKFRKQYSQASPEERVRLLAERETNFYRAHGDDYESVHTWQRQRDNQRSAA
ETTGRRTAAPADGLQDLSVSDVADHRQAGPTRSRDGALLLPSDAHVHVGQSQPGGDSGLRSSVPGGGRGR
RAGSTAERGRGGSQPAAVSQETKGTATPAGATGRRRAGAGKSRNTRVRAGRVVPPYAHNPHRVATIADIE
ERGRRLFDPLKRPSDNALVFYRSSSHPQVADQLPQARAATTTPVQSTAGRRPRSSGKPRPPRQWRPGAVP
PYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVNKHASTVAAYFTRQAQHNQIAPAH
RRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVSVLTFERLKAREQFHKPQPNIEVNLMGSAAIRNLLDD
EKEDPGFSISGARGPGREGVRDQVKRIMDRFAKQVDPDAASERARDLSAKDLYTRKAKFSQNVHYLDKQT
DKTLFVDTGTTISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMN
QSLADRRAELDIERDGQSIGPATDLPRHVDDATRDVRDQADRLGVTVPIEALYGQGKTADQVSQALTAQL
DTVPESERIAFVETVAITLGIPERGQPKGDQAFAQWQAQRAQPAANSAASATSEAQANVPTPSDAVPEPV
IPEAAKPALVNDPDLQSPSELVRLEAQWRRDFPMSEADVRASDTVMGLRGEDHAIWIIATDDKTPEAAAL
LTAYMENDSYREAFKASIVAAYKQVENSPQSVDDLDHLTAMAAQIVNEVEERLFPSPQAATGQTAPSRSK
VIEGTLIDHGEAPYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDADLKQGDQVRLRDLGTQPVVV
QVIEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEDDGMSVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2200 | GenBank | WP_055007056 |
| Name | t4cp2_HTL75_RS00300_pPs0170 |
UniProt ID | _ |
| Length | 553 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 553 a.a. Molecular weight: 61671.27 Da Isoelectric Point: 6.5238
>WP_055007056.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Pseudomonas syringae group]
MARAKSKPISPTNPQKRIEEHPAFLLGKHPTEDSFLASYGQQFVMLAAPPGTGKGVGAVIPNLLSYPDSM
VVNDPKFENWEITSGFRAAAGHKVYRFSPERLETHRWNPLSAINRDPLYRLGEIRTIARVLFVSDNPKNQ
EWYNKAGNVFTAVLLFLMETPEMPFTLPQAYEIGSLGTGMGTWAQQIIELRSTGPNALSFEALRELNGVF
EASKNKSSGWSTTVDIVRDVLSVYAEKTVAWAVSGDDIDLSKAREEKMTAYFSVTEGSLKKYGPLMNLFF
TQAIRLNSKVIPEQGGHCADGTLRYKYQLALLMDELAIMGRIEILETAPALTRGAGLRFFFIFQGKDQLR
AIYGEEAANAIMKAIHNEIVFAPGDIKLAEEYSRRLGNTTVRVHNQSLNRQKHQVGAQGQTDSYSEQPRP
LMLPQDINELPYDKQLIFVQGTKTTPALKILARKIFYYEEEVFRSREKLPPPPLPVGDASKIDALTVPVR
TVEAKVAVADTKPMQAEQRQRWNPRDTKASATEVAQAEADKAQPVEVEPDPEPVQADDTSEPM
MARAKSKPISPTNPQKRIEEHPAFLLGKHPTEDSFLASYGQQFVMLAAPPGTGKGVGAVIPNLLSYPDSM
VVNDPKFENWEITSGFRAAAGHKVYRFSPERLETHRWNPLSAINRDPLYRLGEIRTIARVLFVSDNPKNQ
EWYNKAGNVFTAVLLFLMETPEMPFTLPQAYEIGSLGTGMGTWAQQIIELRSTGPNALSFEALRELNGVF
EASKNKSSGWSTTVDIVRDVLSVYAEKTVAWAVSGDDIDLSKAREEKMTAYFSVTEGSLKKYGPLMNLFF
TQAIRLNSKVIPEQGGHCADGTLRYKYQLALLMDELAIMGRIEILETAPALTRGAGLRFFFIFQGKDQLR
AIYGEEAANAIMKAIHNEIVFAPGDIKLAEEYSRRLGNTTVRVHNQSLNRQKHQVGAQGQTDSYSEQPRP
LMLPQDINELPYDKQLIFVQGTKTTPALKILARKIFYYEEEVFRSREKLPPPPLPVGDASKIDALTVPVR
TVEAKVAVADTKPMQAEQRQRWNPRDTKASATEVAQAEADKAQPVEVEPDPEPVQADDTSEPM
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 39456..49634
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTL75_RS00200 | 34511..35626 | - | 1116 | WP_172689650 | hypothetical protein | - |
| HTL75_RS00205 | 35678..36451 | - | 774 | WP_172689651 | helix-turn-helix transcriptional regulator | - |
| HTL75_RS00210 | 36560..37660 | + | 1101 | WP_172689652 | polyamine ABC transporter substrate-binding protein | - |
| HTL75_RS00215 | 37759..37956 | + | 198 | WP_005782488 | hypothetical protein | - |
| HTL75_RS00220 | 37968..38363 | - | 396 | WP_172689653 | helix-turn-helix transcriptional regulator | - |
| HTL75_RS00225 | 38579..39112 | + | 534 | WP_172689654 | transcription termination/antitermination NusG family protein | - |
| HTL75_RS00230 | 39201..39443 | + | 243 | WP_046720405 | hypothetical protein | - |
| HTL75_RS00235 | 39456..40160 | + | 705 | WP_005782481 | lytic transglycosylase domain-containing protein | virB1 |
| HTL75_RS00240 | 40160..40495 | + | 336 | WP_005782479 | hypothetical protein | - |
| HTL75_RS00245 | 40508..40996 | + | 489 | WP_005782477 | VirB3 family type IV secretion system protein | virB3 |
| HTL75_RS00250 | 40923..43397 | + | 2475 | WP_237183643 | conjugal transfer protein | virb4 |
| HTL75_RS00255 | 43394..44074 | + | 681 | WP_005782474 | P-type DNA transfer protein VirB5 | - |
| HTL75_RS00260 | 44090..44296 | + | 207 | WP_005782472 | hypothetical protein | - |
| HTL75_RS00265 | 44323..45267 | + | 945 | WP_172689656 | type IV secretion system protein | virB6 |
| HTL75_RS00270 | 45649..46437 | + | 789 | WP_010199854 | type IV secretion system protein | virB8 |
| HTL75_RS00275 | 46427..47236 | + | 810 | WP_005782466 | P-type conjugative transfer protein VirB9 | virB9 |
| HTL75_RS00280 | 47223..48593 | + | 1371 | WP_172689657 | TrbI/VirB10 family protein | virB10 |
| HTL75_RS00285 | 48603..49634 | + | 1032 | WP_005782462 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTL75_RS00290 | 49644..50000 | + | 357 | WP_010217013 | hypothetical protein | - |
| HTL75_RS00295 | 50120..50284 | + | 165 | WP_172689642 | hypothetical protein | - |
| HTL75_RS00300 | 50284..51945 | + | 1662 | WP_055007056 | type IV secretory system conjugative DNA transfer family protein | - |
| HTL75_RS00305 | 51986..52348 | + | 363 | WP_172689658 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTL75_RS00310 | 52376..54541 | + | 2166 | WP_172689659 | type IA DNA topoisomerase | - |
Host bacterium
| ID | 3699 | GenBank | NZ_KY362372 |
| Plasmid name | pPs0170 | Incompatibility group | - |
| Plasmid size | 64559 bp | Coordinate of oriT [Strand] | 58231..58410 [+] |
| Host baterium | Pseudomonas syringae pv. syringae strain UMAF0170 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | copS, copR, copC, copA |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |