Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 103255 |
Name | oriT_pEC_18FS090 |
Organism | Escherichia coli strain 18FS090 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_ON960335 (58575..58828 [+], 254 nt) |
oriT length | 254 nt |
IRs (inverted repeats) | 152..157, 159..164 (AAAAGT..ACTTTT) 121..129, 134..142 (TTAAGGCTT..AAGCCTTAA) 50..55, 59..64 (AATTTT..AAAATT) |
Location of nic site | 77..78 |
Conserved sequence flanking the nic site |
TTTGGTTAAA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 254 nt
>oriT_pEC_18FS090
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 2432 | GenBank | WP_000980119 |
Name | TraI_2_PRZ99_RS00325_pEC_18FS090 | UniProt ID | A0A0H3VVI8 |
Length | 1050 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1050 a.a. Molecular weight: 118035.42 Da Isoelectric Point: 4.6054
>WP_000980119.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0H3VVI8 |
T4CP
ID | 2199 | GenBank | WP_001284076 |
Name | traD_PRZ99_RS00320_pEC_18FS090 | UniProt ID | _ |
Length | 694 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 694 a.a. Molecular weight: 78137.93 Da Isoelectric Point: 8.0177
>WP_001284076.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 239324..248531
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
PRZ99_RS01345 (CPHELBEB_00553) | 239324..242005 | - | 2682 | WP_000387412 | TraC family protein | virb4 |
PRZ99_RS01350 (CPHELBEB_00554) | 242014..242964 | - | 951 | WP_001022587 | IncHI-type conjugal transfer lipoprotein TrhV | traV |
PRZ99_RS01355 (CPHELBEB_00555) | 242974..243726 | - | 753 | WP_183079335 | protein-disulfide isomerase HtdT | - |
PRZ99_RS01360 (CPHELBEB_00556) | 243845..244327 | - | 483 | WP_065311104 | transcriptional regulator | - |
PRZ99_RS01365 (CPHELBEB_00557) | 244335..245690 | - | 1356 | WP_000351841 | IncHI-type conjugal transfer protein TrhB | traB |
PRZ99_RS01370 (CPHELBEB_00558) | 245680..246141 | - | 462 | WP_000521240 | plasmid transfer protein HtdO | - |
PRZ99_RS01375 (CPHELBEB_00559) | 246143..247414 | - | 1272 | WP_272487465 | type-F conjugative transfer system secretin TraK | traK |
PRZ99_RS01380 (CPHELBEB_00560) | 247414..248202 | - | 789 | WP_000783153 | TraE/TraK family type IV conjugative transfer system protein | traE |
PRZ99_RS01385 (CPHELBEB_00561) | 248214..248531 | - | 318 | WP_000043356 | type IV conjugative transfer system protein TraL | traL |
PRZ99_RS01390 (CPHELBEB_00562) | 248582..248935 | - | 354 | WP_000423602 | hypothetical protein | - |
PRZ99_RS01395 (CPHELBEB_00565) | 250084..250959 | - | 876 | WP_000594612 | RepB family plasmid replication initiator protein | - |
PRZ99_RS01400 (CPHELBEB_00566) | 251436..252491 | + | 1056 | WP_065311103 | hypothetical protein | - |
PRZ99_RS01405 (CPHELBEB_00567) | 252509..252709 | + | 201 | WP_000594931 | hypothetical protein | - |
PRZ99_RS01410 (CPHELBEB_00568) | 252706..253422 | + | 717 | WP_000838218 | zinc finger-like domain-containing protein | - |
Host bacterium
ID | 3698 | GenBank | NZ_ON960335 |
Plasmid name | pEC_18FS090 | Incompatibility group | - |
Plasmid size | 267485 bp | Coordinate of oriT [Strand] | 58575..58828 [+] |
Host baterium | Escherichia coli strain 18FS090 |
Cargo genes
Drug resistance gene | aph(6)-Id, aph(3'')-Ib, sul2, sul3, lnu(F), ant(3'')-Ia, aac(3)-IId, blaTEM-1B, blaCTX-M-55, qnrS1, mph(A), ARR-3, dfrA14, floR, aph(3')-Ia |
Virulence gene | - |
Metal resistance gene | terE, terD, terC, terB, terA, terZ, terW |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |