Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103255
Name   oriT_pEC_18FS090 in_silico
Organism   Escherichia coli strain 18FS090
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_ON960335 (58575..58828 [+], 254 nt)
oriT length   254 nt
IRs (inverted repeats)      152..157, 159..164  (AAAAGT..ACTTTT)
 121..129, 134..142  (TTAAGGCTT..AAGCCTTAA)
 50..55, 59..64  (AATTTT..AAAATT)
Location of nic site      77..78
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 254 nt

>oriT_pEC_18FS090
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2432 GenBank   WP_000980119
Name   TraI_2_PRZ99_RS00325_pEC_18FS090 insolico UniProt ID   A0A0H3VVI8
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118035.42 Da        Isoelectric Point: 4.6054

>WP_000980119.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A0H3VVI8


T4CP

ID   2199 GenBank   WP_001284076
Name   traD_PRZ99_RS00320_pEC_18FS090 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78137.93 Da        Isoelectric Point: 8.0177

>WP_001284076.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-249)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 239324..248531

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PRZ99_RS01345 (CPHELBEB_00553) 239324..242005 - 2682 WP_000387412 TraC family protein virb4
PRZ99_RS01350 (CPHELBEB_00554) 242014..242964 - 951 WP_001022587 IncHI-type conjugal transfer lipoprotein TrhV traV
PRZ99_RS01355 (CPHELBEB_00555) 242974..243726 - 753 WP_183079335 protein-disulfide isomerase HtdT -
PRZ99_RS01360 (CPHELBEB_00556) 243845..244327 - 483 WP_065311104 transcriptional regulator -
PRZ99_RS01365 (CPHELBEB_00557) 244335..245690 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
PRZ99_RS01370 (CPHELBEB_00558) 245680..246141 - 462 WP_000521240 plasmid transfer protein HtdO -
PRZ99_RS01375 (CPHELBEB_00559) 246143..247414 - 1272 WP_272487465 type-F conjugative transfer system secretin TraK traK
PRZ99_RS01380 (CPHELBEB_00560) 247414..248202 - 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
PRZ99_RS01385 (CPHELBEB_00561) 248214..248531 - 318 WP_000043356 type IV conjugative transfer system protein TraL traL
PRZ99_RS01390 (CPHELBEB_00562) 248582..248935 - 354 WP_000423602 hypothetical protein -
PRZ99_RS01395 (CPHELBEB_00565) 250084..250959 - 876 WP_000594612 RepB family plasmid replication initiator protein -
PRZ99_RS01400 (CPHELBEB_00566) 251436..252491 + 1056 WP_065311103 hypothetical protein -
PRZ99_RS01405 (CPHELBEB_00567) 252509..252709 + 201 WP_000594931 hypothetical protein -
PRZ99_RS01410 (CPHELBEB_00568) 252706..253422 + 717 WP_000838218 zinc finger-like domain-containing protein -

Host bacterium

ID   3698 GenBank   NZ_ON960335
Plasmid name   pEC_18FS090 Incompatibility group   -
Plasmid size   267485 bp Coordinate of oriT [Strand]   58575..58828 [+]
Host baterium   Escherichia coli strain 18FS090

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, sul2, sul3, lnu(F), ant(3'')-Ia, aac(3)-IId, blaTEM-1B, blaCTX-M-55, qnrS1, mph(A), ARR-3, dfrA14, floR, aph(3')-Ia
Virulence gene   -
Metal resistance gene   terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -