Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103254
Name   oriT_pCTX-M_FZKP3931 in_silico
Organism   Klebsiella pneumoniae strain FZKP3931
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OP006451 (39164..39287 [+], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      101..106, 119..124  (TTTAAT..ATTAAA)
 91..99, 113..121  (AATAATGTA..TACATTATT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_pCTX-M_FZKP3931
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTTTTTGAATCATTAGCTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATTAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   2198 GenBank   WP_015059012
Name   traC_PR011_RS00335_pCTX-M_FZKP3931 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99393.22 Da        Isoelectric Point: 6.3465

>WP_015059012.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA
TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-446)

TraG P-loop domain (P-loop_TraG)

(467-771)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 38592..57431

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PR011_RS00230 (GPAHCPHI_00044) 34564..34998 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
PR011_RS00235 (GPAHCPHI_00045) 34995..35714 + 720 WP_001276217 plasmid SOS inhibition protein A -
PR011_RS00240 35936..36085 + 150 Protein_48 plasmid maintenance protein Mok -
PR011_RS00245 36027..36152 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
PR011_RS00250 (GPAHCPHI_00047) 36471..36767 - 297 Protein_50 hypothetical protein -
PR011_RS00255 (GPAHCPHI_00049) 37067..37363 + 297 WP_001272251 hypothetical protein -
PR011_RS00260 (GPAHCPHI_00050) 37474..38295 + 822 WP_001234445 DUF932 domain-containing protein -
PR011_RS00265 (GPAHCPHI_00051) 38592..39239 - 648 WP_015059008 transglycosylase SLT domain-containing protein virB1
PR011_RS00270 (GPAHCPHI_00052) 39516..39899 + 384 WP_000124981 conjugal transfer relaxosome DNA-binding protein TraM -
PR011_RS00275 (GPAHCPHI_00053) 40090..40776 + 687 WP_015059009 PAS domain-containing protein -
PR011_RS00280 (GPAHCPHI_00054) 40870..41097 + 228 WP_001254386 conjugal transfer relaxosome protein TraY -
PR011_RS00285 (GPAHCPHI_00055) 41131..41496 + 366 WP_021519752 type IV conjugative transfer system pilin TraA -
PR011_RS00290 (GPAHCPHI_00056) 41511..41822 + 312 WP_000012106 type IV conjugative transfer system protein TraL traL
PR011_RS00295 (GPAHCPHI_00057) 41844..42410 + 567 WP_000399794 type IV conjugative transfer system protein TraE traE
PR011_RS00300 (GPAHCPHI_00058) 42397..43125 + 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
PR011_RS00305 (GPAHCPHI_00059) 43125..44552 + 1428 WP_032297072 F-type conjugal transfer pilus assembly protein TraB traB
PR011_RS00310 (GPAHCPHI_00060) 44542..45132 + 591 WP_000002778 conjugal transfer pilus-stabilizing protein TraP -
PR011_RS00315 (GPAHCPHI_00061) 45119..45316 + 198 WP_001324648 conjugal transfer protein TrbD -
PR011_RS00320 (GPAHCPHI_00062) 45328..45579 + 252 WP_001038341 conjugal transfer protein TrbG -
PR011_RS00325 (GPAHCPHI_00063) 45576..46091 + 516 WP_062955122 type IV conjugative transfer system lipoprotein TraV traV
PR011_RS00330 (GPAHCPHI_00064) 46226..46447 + 222 WP_001278692 conjugal transfer protein TraR -
PR011_RS00335 (GPAHCPHI_00065) 46607..49234 + 2628 WP_015059012 type IV secretion system protein TraC virb4
PR011_RS00340 (GPAHCPHI_00066) 49231..49617 + 387 WP_015059013 type-F conjugative transfer system protein TrbI -
PR011_RS00345 (GPAHCPHI_00067) 49614..50246 + 633 WP_001203735 type-F conjugative transfer system protein TraW traW
PR011_RS00350 (GPAHCPHI_00068) 50243..51235 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
PR011_RS00355 (GPAHCPHI_00069) 51262..51570 + 309 WP_000412447 hypothetical protein -
PR011_RS00360 (GPAHCPHI_00070) 51633..52154 + 522 WP_015059014 hypothetical protein -
PR011_RS00365 (GPAHCPHI_00071) 52181..52819 + 639 WP_000087532 type-F conjugative transfer system pilin assembly protein TrbC trbC
PR011_RS00370 (GPAHCPHI_00072) 52816..53187 + 372 WP_000056336 hypothetical protein -
PR011_RS00375 (GPAHCPHI_00073) 53213..53635 + 423 WP_001229309 HNH endonuclease signature motif containing protein -
PR011_RS00380 (GPAHCPHI_00074) 53632..55482 + 1851 WP_015059015 type-F conjugative transfer system mating-pair stabilization protein TraN traN
PR011_RS00385 (GPAHCPHI_00075) 55509..55766 + 258 WP_053913405 conjugal transfer protein TrbE -
PR011_RS00390 (GPAHCPHI_00076) 55753..56502 + 750 WP_072354014 type-F conjugative transfer system pilin assembly protein TraF traF
PR011_RS00395 (GPAHCPHI_00077) 56663..56899 + 237 WP_000270010 type-F conjugative transfer system pilin chaperone TraQ -
PR011_RS00400 (GPAHCPHI_00078) 56886..57431 + 546 WP_000059817 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
PR011_RS00405 (GPAHCPHI_00079) 57421..57735 + 315 WP_001384255 P-type conjugative transfer protein TrbJ -
PR011_RS00410 (GPAHCPHI_00080) 57689..58081 + 393 WP_000126338 F-type conjugal transfer protein TrbF -
PR011_RS00415 (GPAHCPHI_00081) 58068..58874 + 807 Protein_83 conjugal transfer protein TraH -
PR011_RS00420 (GPAHCPHI_00082) 58927..59631 - 705 WP_001067855 IS6-like element IS26 family transposase -
PR011_RS00425 (GPAHCPHI_00083) 60100..60663 - 564 WP_155082050 hypothetical protein -
PR011_RS00430 60785..61327 - 543 WP_077273850 restriction endonuclease -
PR011_RS00435 (GPAHCPHI_00085) 61309..62289 - 981 WP_000019445 IS5-like element ISKpn26 family transposase -

Host bacterium

ID   3697 GenBank   NZ_OP006451
Plasmid name   pCTX-M_FZKP3931 Incompatibility group   IncR
Plasmid size   136951 bp Coordinate of oriT [Strand]   39164..39287 [+]
Host baterium   Klebsiella pneumoniae strain FZKP3931

Cargo genes

Drug resistance gene   blaCTX-M-65, blaTEM-1B, rmtB
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9